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Literature summary for 2.7.1.1 extracted from
- Function of interdomain alpha-helix in human brain hexokinase: covalent linkage and catalytic regulation between N- and C-terminal halves (2007), J. Biomed. Sci., 14, 195-202.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A464P/E465G | mutant with a helix breaker embedded in the interdomain alpha-helix has a smaller magnitude of phosphate alleviation than the wild type | Homo sapiens |
| Q466_H467insHMNLAEQ | mutant has a smaller magnitude of phosphate alleviation than the wild type | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,5-Anhydro-D-glucitol 6-phosphate | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
D-glucose | pH 8.0, mutant enzyme A464P/E465G | Homo sapiens | |
| 0.0732 | - |
D-glucose | pH 8.0, wild-type enzyme | Homo sapiens | |
| 0.0841 | - |
D-glucose | pH 8.0, mutant enzyme Q466_H467insHMNLAEQ | Homo sapiens | |
| 1.09 | - |
ATP | pH 8.0, wild-type enzyme | Homo sapiens | |
| 1.16 | - |
ATP | pH 8.0, mutant enzyme A464P/E465G | Homo sapiens | |
| 1.51 | - |
ATP | pH 8.0, mutant enzyme Q466_H467insHMNLAEQ | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0107 | - |
1,5-Anhydro-D-glucitol 6-phosphate | pH 8.0, mutant enzyme A464P/E465G | Homo sapiens | |
| 0.0108 | - |
1,5-Anhydro-D-glucitol 6-phosphate | pH 8.0, mutant enzyme Q466_H467insHMNLAEQ | Homo sapiens | |
| 0.0164 | - |
1,5-Anhydro-D-glucitol 6-phosphate | pH 8.0, wild-type enzyme | Homo sapiens | |
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