Protein Variants | Comment | Organism |
---|---|---|
A456V | mutant enzyme is as stable as the wild-type enzyme. Ability of glucokinase regulatory protein to inhibit mutant enzyme A456V is less than that of the wild type enzyme. Mutant shows 37.9% of the wild-type activity. KM-value for ATP is 1.3fold lower than wild-type value. Glucokinase A456V/A456V mice are viable and have similar plasma triglycerides, free fatty acid levels, and similar or slightly lower body weight when compared with control mice | Mus musculus |
K414E | mutant enzyme is thermolabile. Mutant shows 0.32% of the wild-type activity. KM-value for ATP is 4.4fold higher than wild-type value. Glucokinase K414E/K414E mice die within a few days after birth of severe hyperglycemia | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
ATP | 37°C, pH 7.4, mutant enzyme A456V | Mus musculus | |
0.39 | - |
ATP | 37°C, pH 7.4, wild-type enzyme | Mus musculus | |
1.73 | - |
ATP | 37°C, pH 7.4, mutant enzyme K414E | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Mus musculus | ADP + D-glucose 6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | ATP | 37°C, pH 7.4, mutant enzyme A456V | Mus musculus | |
2 | 3.7 | D-glucose | 37°C, pH 7.4, mutant enzyme A456V | Mus musculus | |
11 | - |
ATP | 37°C, pH 7.4, mutant enzyme K414E | Mus musculus | |
11 | - |
D-glucose | 37°C, pH 7.4, mutant enzyme K414E | Mus musculus | |
53.2 | - |
ATP | 37°C, pH 7.4, wild-type enzyme | Mus musculus | |
53.2 | - |
D-glucose | 37°C, pH 7.4, wild-type enzyme | Mus musculus | |
84.9 | - |
ATP | 37°C, pH 7.4, mutant enzyme A456V | Mus musculus | |
84.9 | - |
D-glucose | 37°C, pH 7.4, mutant enzyme A456V | Mus musculus |