Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive versus ATP, both isozymes HKI and HKII | Brassica rapa subsp. oleifera | |
AMP | competitive versus ATP, both isozymes HKI and HKII | Brassica rapa subsp. oleifera | |
D-fructose 6-phosphate | product inhibition, both isozymes HKI and HKII | Brassica rapa subsp. oleifera | |
D-glucose 6-phosphate | product inhibition, both isozymes HKI and HKII | Brassica rapa subsp. oleifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
D-fructose | isozyme HKII | Brassica rapa subsp. oleifera | |
1.25 | - |
D-glucose | isozyme HKI | Brassica rapa subsp. oleifera | |
2.5 | - |
D-fructose | isozyme HKI | Brassica rapa subsp. oleifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required for activity by both isozymes HKI and HKII | Brassica rapa subsp. oleifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
isozymes HKI and HKII, gel filtration | Brassica rapa subsp. oleifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica rapa subsp. oleifera | - |
2 isozymes HKI and HKII | - |
Purification (Comment) | Organism |
---|---|
isozymes HKI and HKII, 170fold, from immature seeds, by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration | Brassica rapa subsp. oleifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | isozyme HKI, isozyme HKII is highly specific for D-fructose | Brassica rapa subsp. oleifera | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-glucose | isozyme HKI | Brassica rapa subsp. oleifera | ADP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, isozymes HKI and HKII, SDS-PAGE | Brassica rapa subsp. oleifera |
Synonyms | Comment | Organism |
---|---|---|
HK | - |
Brassica rapa subsp. oleifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
isozyme HKI | Brassica rapa subsp. oleifera |
9 | - |
isozyme HKII | Brassica rapa subsp. oleifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | preferred by both isozymes HKI and HKII | Brassica rapa subsp. oleifera |