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Literature summary for 2.7.1.1 extracted from

  • Jangra, A.P.; Malhotra, S.P.; Saharan, M.R.; Singh, R.
    Purification and characterization of hexokinase from developing seeds of Brassica campestris L. (2004), Physiol. Mol. Biol. Plants, 10, 217-222.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive versus ATP, both isozymes HKI and HKII Brassica rapa subsp. oleifera
AMP competitive versus ATP, both isozymes HKI and HKII Brassica rapa subsp. oleifera
D-fructose 6-phosphate product inhibition, both isozymes HKI and HKII Brassica rapa subsp. oleifera
D-glucose 6-phosphate product inhibition, both isozymes HKI and HKII Brassica rapa subsp. oleifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
D-fructose isozyme HKII Brassica rapa subsp. oleifera
1.25
-
D-glucose isozyme HKI Brassica rapa subsp. oleifera
2.5
-
D-fructose isozyme HKI Brassica rapa subsp. oleifera

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely required for activity by both isozymes HKI and HKII Brassica rapa subsp. oleifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
isozymes HKI and HKII, gel filtration Brassica rapa subsp. oleifera

Organism

Organism UniProt Comment Textmining
Brassica rapa subsp. oleifera
-
2 isozymes HKI and HKII
-

Purification (Commentary)

Purification (Comment) Organism
isozymes HKI and HKII, 170fold, from immature seeds, by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration Brassica rapa subsp. oleifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose isozyme HKI, isozyme HKII is highly specific for D-fructose Brassica rapa subsp. oleifera ADP + D-fructose 6-phosphate
-
?
ATP + D-glucose isozyme HKI Brassica rapa subsp. oleifera ADP + D-glucose 6-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47000, isozymes HKI and HKII, SDS-PAGE Brassica rapa subsp. oleifera

Synonyms

Synonyms Comment Organism
HK
-
Brassica rapa subsp. oleifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
isozyme HKI Brassica rapa subsp. oleifera
9
-
isozyme HKII Brassica rapa subsp. oleifera

Cofactor

Cofactor Comment Organism Structure
ATP preferred by both isozymes HKI and HKII Brassica rapa subsp. oleifera