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Literature summary for 2.7.1.1 extracted from

  • Xu, L.Z.; Harrison, R.W.; Weber, I.T.; Pilkis, S.J.
    Human beta-cell glucokinase. Dual role of Ser-151 in catalysis and hexose affinity (1995), J. Biol. Chem., 270, 9939-9946.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
N166R increased affinity for glucose and ATP by a factor of 3 Homo sapiens
N166R-S151-A lowers the KM-value for glucose by a factor of 40 and increases the KM-value for ATP Homo sapiens
S151A lowers the KM-value for glucose by a factor of 26, increases the KM-value for ATP and decreases the KM-value for mannose and fructose Homo sapiens
S151C lowers the KM-value for glucose by a factor of 2, increases the KM-value for ATP and decreases the KM-value for mannose and fructose Homo sapiens
S151G lowers the KM-value for glucose by a factor of 40, increases the KM-value for ATP and decreases the KM-value for mannose and fructose Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 6-phosphate
-
 Homo sapiens
N-acetylglucosamine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 D-mannose 30°C, pH 7.5, N166R-S151A Homo sapiens
0.12
-
 D-mannose 30°C, pH 7.5, S151G Homo sapiens
0.15
-
 D-glucose 30°C, pH 7.5, S151G and N1166R-S151A Homo sapiens
0.21
-
 D-mannose 30°C, pH 7.5, S151A Homo sapiens
0.27
-
 ATP 30°C, pH 7.5, N166R Homo sapiens
0.34
-
 D-glucose 30°C, pH 7.5, S151A Homo sapiens
0.63
-
 ATP 30°C, pH 7.5, wild-type Homo sapiens
1.52
-
 ATP 30°C, pH 7.5, N166R-S151A Homo sapiens
1.9
-
 2-deoxy-D-glucose 30°C, pH 7.5, S151A Homo sapiens
2.1
-
 D-glucose 30°C, pH 7.5, S151C and N166R Homo sapiens
2.2
-
 D-mannose 30°C, pH 7.5, N166R Homo sapiens
2.21
-
 ATP 30°C, pH 7.5, S151A Homo sapiens
2.26
-
 ATP 30°C, pH 7.5, S151G Homo sapiens
2.6
-
 ATP 30°C, pH 7.5, S151C Homo sapiens
3.74
-
 D-mannose 30°C, pH 7.5, S151C Homo sapiens
4.4
-
 D-mannose 30°C, pH 7.5, wild-type Homo sapiens
4.7
-
 D-fructose 30°C, pH 7.5, N166R-S151A Homo sapiens
6
-
 D-glucose 30°C, pH 7.5, wild-type Homo sapiens
7.7
-
 D-fructose 30°C, pH 7.5, S151G Homo sapiens
18
-
 2-deoxyglucose 30°C, pH 7.5, wild-type Homo sapiens
57.3
-
 D-fructose 30°C, pH 7.5, S151A Homo sapiens
77
-
 D-fructose 30°C, pH 7.5, N166R Homo sapiens
240
-
 D-fructose 30°C, pH 7.5, wild-type and S151C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Homo sapiens
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
pancreatic islet beta-cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.34
-
 S151G, D-mannose as substrate Homo sapiens
0.6
-
 S151G, D-glucose as substrate Homo sapiens
1.4
-
 N166R-S151A, D-mannose as substrate Homo sapiens
2
-
 N166R-S151A, D-glucose as substrate Homo sapiens
2.16
-
 S151C, D-mannose as substrate Homo sapiens
3.18
-
 S151G, D-fructose as substrate Homo sapiens
3.32
-
 S151A, D-mannose as substrate Homo sapiens
4.1
-
 S151A, 2-deoxy-D-glucose as substrate Homo sapiens
6
-
 S151C, D-glucose as substrate Homo sapiens
6.6
-
 S151A, D-glucose as substrate Homo sapiens
9.12
-
 S151C, D-fructose as substrate Homo sapiens
14.5
-
 N166R-S151A, D-fructose as substrate Homo sapiens
23.8
-
 S151A, D-fructose as substrate Homo sapiens
60.8
-
 N166R, D-mannose as substrate Homo sapiens
68
-
 wild-type, D-mannose and 2-deoxy-D-glucose as substrate Homo sapiens
80
-
 wild-type and N166R, D-glucose as substrate Homo sapiens
122
-
 wild-type, D-fructose as substrate Homo sapiens
310
-
 N166R, D-fructose as substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose
-
 Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
ATP + D-fructose
-
 Homo sapiens ADP + D-fructose 6-phosphate
-
 ?
ATP + D-glucose
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?
ATP + D-mannose
-
 Homo sapiens ADP + D-mannose 6-phosphate
-
 ?