Any feedback?
Literature summary for 2.7.1.1 extracted from
- The first archaeal ATP-dependent glucokinase, from the hyperthermophilic crenarchaeon Aeropyrum pernix, represents a monomeric, extremely thermophilic ROK glucokinase with broad hexose specificity (2002), J. Bacteriol., 184, 5955-5965.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aeropyrum pernix |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
D-glucose | 90°C, pH 6.2 | Aeropyrum pernix |  |
| 0.06 | - |
D-glucosamine | 90°C, pH 6.2 | Aeropyrum pernix | |
| 0.11 | - |
D-mannose | 90°C, pH 6.2 | Aeropyrum pernix | |
| 0.15 | - |
D-fructose | 90°C, pH 6.2 | Aeropyrum pernix | |
| 0.33 | - |
2-deoxy-D-glucose | 90°C, pH 6.2 | Aeropyrum pernix | |
| 0.42 | - |
ATP | 90°C, pH 6.2 | Aeropyrum pernix | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Aeropyrum pernix | |
| Mg2+ | required | Aeropyrum pernix | |
| Mn2+ | - |
Aeropyrum pernix | |
| Ni2+ | - |
Aeropyrum pernix | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | 33000 | gel filtration | Aeropyrum pernix |
| 36000 | - |
1 * 36000, SDS-PAGE | Aeropyrum pernix |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Aeropyrum pernix | - |
ADP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aeropyrum pernix |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15 | - |
D-fructose as substrate | Aeropyrum pernix |
| 19 | - |
D-mannose as substrate | Aeropyrum pernix |
| 27 | - |
D-glucosamine as substrate | Aeropyrum pernix |
| 35 | - |
D-glucose as substrate | Aeropyrum pernix |
| 45 | - |
2-deoxy-D-glucose as substrate | Aeropyrum pernix |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-glucose | - |
Aeropyrum pernix | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| ATP + D-fructose | - |
Aeropyrum pernix | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-glucosamine | - |
Aeropyrum pernix | ADP + D-glucosamine 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Aeropyrum pernix | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-mannose | - |
Aeropyrum pernix | ADP + D-mannose 6-phosphate | - |
? | |
| GTP + D-glucose | - |
Aeropyrum pernix | GDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | - |
Aeropyrum pernix | IDP + D-glucose 6-phosphate | - |
? | |
| UTP + D-glucose | - |
Aeropyrum pernix | UDP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE | Aeropyrum pernix |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Aeropyrum pernix |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
stable up to 100°C | Aeropyrum pernix |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
