Application | Comment | Organism |
---|---|---|
pharmacology | enzyme may constitute an interesting drug target | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
hexokinase gene is cloned, sequenced and expressed in Escherichia coli XL-1 Blue, gene encodes a polypeptide of 471 amino acids | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | natural hexokinase from epimastigotes | Trypanosoma cruzi | |
diphosphate | natural hexokinase from epimastigotes, mixed-type inhibition | Trypanosoma cruzi | |
additional information | not inhibited by D-fructose; not inhibited by D-glucose 6-phosphate; not inhibited by trehalose 6-phosphate | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
D-glucose | natural hexokinase from epimastigotes, at pH 7.5 | Trypanosoma cruzi | |
0.06 | - |
D-glucose | recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at pH 7.5 | Trypanosoma cruzi | |
0.32 | 0.38 | ATP | natural and recombinant hexokinase, expressed in Escherichia coli XL-1 Blue, at pH 7.5 | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | in glycosome-enriched fraction | Trypanosoma cruzi | 20015 | - |
additional information | sequence contains an N-terminal peroxisome-targeting signal, PTS-2, subcellular distribution | Trypanosoma cruzi | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgCl2 is most effective, followed by MnCl2 with 16% of the activity found with MgCl2 | Trypanosoma cruzi | |
Mg2+ | strict requirement for divalent metal ions | Trypanosoma cruzi | |
Mn2+ | MgCl2 is most effective, followed by MnCl2 with 16% of the activity found with MgCl2 | Trypanosoma cruzi | |
Mn2+ | strict requirement for divalent metal ions | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, at high ionic strength the enzyme exists as dimer, SDS-PAGE | Trypanosoma cruzi |
49000 | - |
4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE | Trypanosoma cruzi |
52000 | - |
4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE | Trypanosoma cruzi |
100000 | - |
natural hexokinase, dimer, at high ionic strength, enzyme exists in several aggregation states | Trypanosoma cruzi |
180000 | - |
recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at low ionic strength, gel filtration | Trypanosoma cruzi |
200000 | - |
gel filtration | Trypanosoma cruzi |
200000 | - |
natural hexokinase, tetramer, at low ionic strength, enzyme exists in several aggregation states | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Trypanosoma cruzi | glycolytic enzyme | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q8ST54 | strains Y and EP, similar to hexokinase IV | - |
Purification (Comment) | Organism |
---|---|
48.6fold: natural hexokinase from epimastigotes, 66.3fold: recombinant hexokinase expressed in Escherichia coli XL-1 Blue | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.27 | - |
recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at 25°C, pH 7.5 | Trypanosoma cruzi |
19.44 | - |
natural hexokinase from epimastigotes, at 25°C, pH 7.5 | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | specific for glucose | Trypanosoma cruzi | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | glycolytic enzyme | Trypanosoma cruzi | D-glucose 6-phosphate + ADP | - |
? | |
additional information | not: diphosphate | Trypanosoma cruzi | ? | - |
? | |
additional information | not: mannose | Trypanosoma cruzi | ? | - |
? | |
additional information | not: fructose | Trypanosoma cruzi | ? | - |
? | |
additional information | not: D-galactose | Trypanosoma cruzi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49000, at high ionic strength the enzyme exists as dimer, SDS-PAGE | Trypanosoma cruzi |
tetramer | 4 * 52000, predominant form, enzyme exists in several aggregation states, calculated from the amino acid sequence, 4 * 49000, SDS-PAGE | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | natural hexokinase: 68160/min, recombinant hexokinase expressed in Escherichia coli XL-1 Blue: 18913/min, at pH 7.5 | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trypanosoma cruzi |
7.5 | 8.8 | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
diphosphate | natural hexokinase from epimastigotes, at pH 7.5 | Trypanosoma cruzi | |
0.7 | - |
diphosphate | recombinant hexokinase expressed in Escherichia coli, at pH 7.5 | Trypanosoma cruzi | |
1.3 | - |
ADP | natural hexokinase from epimastigotes, at pH 7.5 | Trypanosoma cruzi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trypanosoma cruzi | calculated basic isoelectric point | - |
9.67 |