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Literature summary for 2.7.1.1 extracted from

  • Saito, T.; Maeda, T.; Nakazawa, M.; Takeuchi, T.; Nozaki, T.; Asai, T.
    Characterisation of hexokinase in Toxoplasma gondii tachyzoites (2002), Int. J. Parasitol., 32, 961-967.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
hexokinase gene is cloned and expressed in Escherichia coli BL21, amino acid sequence, single copy gene Toxoplasma gondii

General Stability

General Stability Organism
one cycle of freezing and thawing, no loss of activity Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by D-fructose 6-phosphate, ADP; not inhibited by D-glucose 6-phosphate; not inhibited by trehalose 6-phosphate Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 D-glucose at 37°C, pH 7 Toxoplasma gondii
1.05
-
 ATP at 37°C, pH 7 Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol in both cytosol and membrane fractions, hexokinase weakly associates with the membrane or particulate fraction Toxoplasma gondii 5829
-
membrane in both cytosol and membrane fractions, hexokinase weakly associates with the membrane or particulate fraction Toxoplasma gondii 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, SDS-PAGE, x * 51465, calculated from the amino acid sequence Toxoplasma gondii
51465
-
 x * 51000, SDS-PAGE, x * 51465, calculated from the amino acid sequence Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Toxoplasma gondii first step of the glycolytic pathway, physiological function of membrane-bound hexokinase in the tachyzoite: glucose channeling with a glucose/hexose transporter D-glucose 6-phosphate + ADP
-
 ?
D-glucose + ATP Toxoplasma gondii rate-limiting step in the glycolysis D-glucose 6-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii Q969A8 RH strain
-
Toxoplasma gondii RH Q969A8 RH strain
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information a posttranslational modification leading to membrane binding may occur Toxoplasma gondii

Purification (Commentary)

Purification (Comment) Organism
recombinant hexokinase, expressed in Escherichia coli BL21 Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
 Toxoplasma gondii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.04
-
 recombinant hexokinase, expressed in Escherichia coli BL21, at 37°C, pH 7 Toxoplasma gondii

Storage Stability

Storage Stability Organism
4°C, 20 mM Tris-HCl, pH 8, 30% glycerol, 100 mM NaCl, 2 mM CaCl2, 0.1 mM EDTA, 1 mM dithiothreitol, 1 week, about 10% loss of activity Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + ATP 79% of the activity with glucose Toxoplasma gondii ADP + D-fructose 6-phosphate
-
 ?
D-glucose + ATP specific for ATP Toxoplasma gondii ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP preferred specificity for glucose Toxoplasma gondii ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP first step of the glycolytic pathway, physiological function of membrane-bound hexokinase in the tachyzoite: glucose channeling with a glucose/hexose transporter Toxoplasma gondii D-glucose 6-phosphate + ADP
-
 ?
D-glucose + ATP rate-limiting step in the glycolysis Toxoplasma gondii D-glucose 6-phosphate + ADP
-
 ?
D-mannose + ATP 55% of the activity with glucose Toxoplasma gondii ADP + D-mannose 6-phosphate
-
 ?
additional information not: ADP Toxoplasma gondii ?
-
 ?
additional information not: UTP Toxoplasma gondii ?
-
 ?
additional information not: diphosphate Toxoplasma gondii ?
-
 ?
additional information not: D-galactose Toxoplasma gondii ?
-
 ?
additional information not: ITP Toxoplasma gondii ?
-
 ?
additional information not: GTP Toxoplasma gondii ?
-
 ?
additional information not: CTP Toxoplasma gondii ?
-
 ?
additional information not: ADP Toxoplasma gondii RH ?
-
 ?
additional information not: UTP Toxoplasma gondii RH ?
-
 ?
additional information not: diphosphate Toxoplasma gondii RH ?
-
 ?
additional information not: D-galactose Toxoplasma gondii RH ?
-
 ?
additional information not: ITP Toxoplasma gondii RH ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE, x * 51465, calculated from the amino acid sequence Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Toxoplasma gondii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.32
-
 ATP at 37°C, pH 7 Toxoplasma gondii
3.47
-
 D-glucose at 37°C, pH 7 Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Toxoplasma gondii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 more than 80% of the maximal activity between pH 6.5 and 8 Toxoplasma gondii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Toxoplasma gondii

pI Value

Organism Comment pI Value Maximum pI Value
Toxoplasma gondii
-
-
 5.82