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Literature summary for 2.7.1.1 extracted from

  • Petit, T.; Blazquez, M.A.; Gancedo, C.
    Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: Biochemical and molecular characterization of both hexokinases (1996), FEBS Lett., 378, 185-189.
    View publication on PubMed
Search for more literature for 2.7.1.1

Activating Compound

Activating Compound Comment Organism Structure
additional information hxk1+ expression increases strongly during growth in fructose or glycerol Schizosaccharomyces pombe
additional information hxk2+ expression is highest during growth in glycerol Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment) Organism
genes hxk1+ and hxk2+, encoding hexokinases 1 and 2, are cloned, sequenced and expressed in Saccharomyces cerevisiae WF8-2C Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-D-glucose
-
 Schizosaccharomyces pombe
D-glucosamine
-
 Schizosaccharomyces pombe
additional information not inhibited by trehalose 6-phosphate; not inhibited by trehalose 6-phosphate Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 D-mannose
-
 Schizosaccharomyces pombe
0.13
-
 D-mannose
-
 Schizosaccharomyces pombe
0.16
-
 D-glucose
-
 Schizosaccharomyces pombe
1.5
-
 D-fructose
-
 Schizosaccharomyces pombe
8.4 8.5 D-glucose
-
 Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Schizosaccharomyces pombe
53000
-
 gel filtration Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe P50521 hexokinase 2
-
Schizosaccharomyces pombe Q09756 hexokinase 1
-
Schizosaccharomyces pombe PB003 Q09756 hexokinase 1
-
Schizosaccharomyces pombe PB004 P50521 hexokinase 2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe ADP + D-fructose 6-phosphate
-
 ?
D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe PB003 ADP + D-fructose 6-phosphate
-
 ?
D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe PB004 ADP + D-fructose 6-phosphate
-
 ?
D-glucose + ATP 11% of the activity with fructose Schizosaccharomyces pombe ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP 59% of the activity with fructose Schizosaccharomyces pombe ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP 11% of the activity with fructose Schizosaccharomyces pombe PB003 ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP 59% of the activity with fructose Schizosaccharomyces pombe PB004 ADP + D-glucose 6-phosphate
-
 ?
D-mannose + ATP 42% of the activity with fructose Schizosaccharomyces pombe ADP + D-mannose 6-phosphate
-
 ?
D-mannose + ATP 23% of the activity with fructose Schizosaccharomyces pombe ADP + D-mannose 6-phosphate
-
 ?
D-mannose + ATP 23% of the activity with fructose Schizosaccharomyces pombe PB003 ADP + D-mannose 6-phosphate
-
 ?
D-mannose + ATP 42% of the activity with fructose Schizosaccharomyces pombe PB004 ADP + D-mannose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, calculated from the amino acid sequence Schizosaccharomyces pombe
monomer 1 * 53000, calculated from the amino acid sequence Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Schizosaccharomyces pombe

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
 2-deoxy-D-glucose fructose as substrate Schizosaccharomyces pombe
2.1
-
 D-glucosamine fructose as substrate Schizosaccharomyces pombe
4
-
 2-deoxy-D-glucose fructose as substrate Schizosaccharomyces pombe
11
-
 D-glucosamine fructose as substrate Schizosaccharomyces pombe