Cloned (Comment) | Organism |
---|---|
hexokinase gene is cloned by cross hybridization with a probe from the Saccharomyces cerevisiae HXK2 gene and characterized, DNA is sequenced | Schwanniomyces occidentalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutant is unable to grow on fructose as the sole carbon source, but still grows on glucose, deletion mutants prevent glucose repression of invertase and maltase | Schwanniomyces occidentalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
D-glucose | hexokinase | Schwanniomyces occidentalis | |
9.3 | - |
D-fructose | hexokinase | Schwanniomyces occidentalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53400 | - |
x * 53400, calculated from the DNA sequence | Schwanniomyces occidentalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schwanniomyces occidentalis | - |
starch-utilizing yeast, strain CBS819, i.e. ATCC2322, 2 isoenzymes: hexokinase and glucokinase | - |
Purification (Comment) | Organism |
---|---|
hexokinase and glucokinase | Schwanniomyces occidentalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | - |
D-fructose as substrate, hxk mutant | Schwanniomyces occidentalis |
0.076 | - |
D-glucose as substrate, hxk mutant | Schwanniomyces occidentalis |
0.151 | - |
D-glucose as substrate, wild type hexokinase | Schwanniomyces occidentalis |
0.197 | - |
D-fructose as substrate, wild type hexokinase | Schwanniomyces occidentalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + ATP | hexokinase, but not glucokinase | Schwanniomyces occidentalis | ADP + D-fructose 6-phosphate | - |
? | |
D-glucose + ATP | hexokinase and glucokinase | Schwanniomyces occidentalis | ADP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53400, calculated from the DNA sequence | Schwanniomyces occidentalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
hexokinase is extremely unstable at its isoelectric point | Schwanniomyces occidentalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Schwanniomyces occidentalis |