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Literature summary for 2.7.1.1 extracted from

  • Stocchi, V.; Magnani, M.; Novelli, G.; Dacha, M.; Fornaini, G.
    Pig red blood cell hexokinase: Evidence for the presence of hexokinase types II and III, and their purification and characterization (1983), Arch. Biochem. Biophys., 226, 365-376.
    View publication on PubMed
Search for more literature for 2.7.1.1

General Stability

General Stability Organism
D-glucose, D-fructose, D-mannose and high concentrations of glycerol, 10-15% v/v, stabilize the purified enzyme Sus scrofa
sulfhydryl protecting agents, e.g. DTT or 2-mercaptoethanol, required for optimum stability Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
D-glucose higher than 0.4 mM; inhibits hexokinase III Sus scrofa
D-glucose 6-phosphate
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 D-mannose hexokinase III, at 30°C, pH 8.1 Sus scrofa
0.015
-
 D-glucose hexokinase III, at 30°C, pH 8.1 Sus scrofa
0.033
-
 2-deoxy-D-glucose hexokinase III, at 30°C, pH 8.1 Sus scrofa
0.15
-
 D-glucose hexokinase II, at 30°C, pH 8.1 Sus scrofa
0.2
-
 D-glucosamine hexokinase III, at 30°C, pH 8.1 Sus scrofa
0.3
-
 ATP hexokinase II, at 30°C, pH 8.1 Sus scrofa
0.5
-
 ATP hexokinase III, at 30°C, pH 8.1 Sus scrofa
1.5
-
 D-fructose hexokinase III, at 30°C, pH 8.1 Sus scrofa
2.5
-
 ITP hexokinase III, at 30°C, pH 8.1 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Sus scrofa
100000
-
 hexokinases II and III Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 adult pigs
-
Sus scrofa
-
 2 enzyme forms: hexokinase type II and type III
-

Purification (Commentary)

Purification (Comment) Organism
hexokinase III: 290000fold, hexokinase II: partial, 39160fold Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte hexokinase types II and III, hexokinase III is the predominant form in adult pig erythrocytes, hexokinase II in newborn pig erythrocytes Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
 hexokinase type II, at 37°C, pH 8.1 Sus scrofa
191.5
-
 hexokinase type III, at 37°C, pH 8.1 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose hexokinase III: 151% of the activity with glucose Sus scrofa ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
CTP + D-glucose
-
 Sus scrofa CDP + D-glucose 6-phosphate
-
 ?
D-fructose + ATP hexokinase III: 12.6% of the activity with glucose Sus scrofa ADP + D-fructose 6-phosphate
-
 ?
D-glucosamine + ATP hexokinase III: 35% of the activity with glucose Sus scrofa ADP + D-glucosamine 6-phosphate
-
 ?
D-glucose + ATP
-
 Sus scrofa ADP + D-glucose 6-phosphate
-
 ?
D-mannose + ATP hexokinase III: 85% of the activity with glucose Sus scrofa ADP + D-mannose 6-phosphate
-
 ?
ITP + D-glucose hexokinase III: 24% of the activity with glucose Sus scrofa IDP + D-glucose 6-phosphate
-
 ?
ITP + D-glucose MgITP Sus scrofa IDP + D-glucose 6-phosphate
-
 ?
additional information not: UTP Sus scrofa ?
-
 ?
additional information not: GTP Sus scrofa ?
-
 ?
additional information not: CTP Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, hexokinase III, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
 5 mM sodium phosphate buffer, pH 7.5, 0.25 mM glucose, 10% v/v glycerol, 1 mM dithiothreitol, inactivation as a function of time, hexokinase type III is more stable than hexokinase type II Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 hexokinase type III Sus scrofa
9
-
 hexokinase type II Sus scrofa

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Sus scrofa

pI Value

Organism Comment pI Value Maximum pI Value
Sus scrofa hexokinase III, isoelectric focusing 4.4 4.3
Sus scrofa hexokinase II, isoelectric focusing
-
 4.8