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Literature summary for 2.7.1.1 extracted from

  • Magnani, M.; Stocchi, V.; Serafini, G.; Chiarantini, L.; Fornaini, G.
    Purification, properties, and evidence for two subtypes of human placenta hexokinase type I (1988), Arch. Biochem. Biophys., 260, 388-399.
    View publication on PubMed
Search for more literature for 2.7.1.1

General Stability

General Stability Organism
stability depends on the presence of 1 mM D-glucose, 3 mM mercaptoethanol, and 9% v/v glycerol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cl- reversible, 0.25 M NaCl or KCl: 50% inhibition, at a comparable concentration of LiCl: 30% inhibition Homo sapiens
I- at about 0.25 M KI, 70% inhibition, reversible Homo sapiens
KSCN at about 0.25 M, 90% inhibition, reversible Homo sapiens
NO3- at about 0.25 M NaNO3, 40% inhibition, reversible Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 D-glucose hexokinase I, at 37°C, pH 8.1 Homo sapiens
0.1
-
 D-mannose at 37°C, pH 8.1 Homo sapiens
0.1
-
 D-mannose hexokinase I Homo sapiens
0.5
-
 2-deoxy-D-glucose hexokinase I, at 37°C, pH 8.1 Homo sapiens
0.6
-
 D-glucosamine hexokinase I, at 37°C, pH 8.1 Homo sapiens
0.6
-
 ATP MgATP, hexokinase I, at 37°C, pH 8.1 Homo sapiens
5
-
 UTP MgUTP2-, hexokinase I, at 37°C, pH 8.1 Homo sapiens
5
-
 CTP MgCTP2-, hexokinase I, at 37°C, pH 8.1 Homo sapiens
11.4
-
 D-fructose hexokinase I, at 37°C, pH 8.1 Homo sapiens
16.6
-
 ITP MgITP2-, hexokinase I, at 37°C, pH 8.1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion 15% of total placenta hexokinase activity, only hexokinase I Homo sapiens 5739
-
soluble 85% of total placenta hexokinase activity is soluble, of which 70% is hexokinase type I and 30% hexokinase type II Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103000
-
 1 * 103000 or 1 * 112000, 2 subtypes of hexokinase type I, SDS-PAGE Homo sapiens
112000
-
 1 * 103000 or 1 * 112000, 2 subtypes of hexokinase type I, SDS-PAGE Homo sapiens
118000
-
 both subtypes of hexokinase type I, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Homo sapiens glucose metabolism D-glucose 6-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 2 subtypes of hexokinase type I
-

Purification (Commentary)

Purification (Comment) Organism
soluble hexokinase type I with 2 unseparated subtypes, 11600fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
192.5
-
 at 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose hexokinase I: as effective as glucose Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
CTP + D-glucose MgCTP, hexokinase I: 5% of the activity with ATP Homo sapiens CDP + D-glucose 6-phosphate
-
 ?
D-fructose + ATP hexokinase I: 130% of the activity with glucose Homo sapiens ADP + D-fructose 6-phosphate
-
 ?
D-glucosamine + ATP hexokinase I: 40% of the activity with glucose Homo sapiens ADP + D-glucosamine 6-phosphate
-
 ?
D-glucose + ATP
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?
D-glucose + ATP glucose metabolism Homo sapiens D-glucose 6-phosphate + ADP
-
 ?
D-mannose + ATP hexokinase I: 106% of the activity with glucose Homo sapiens ADP + D-mannose 6-phosphate
-
 ?
ITP + D-glucose MgITP Homo sapiens IDP + D-glucose 6-phosphate
-
 ?
ITP + D-glucose hexokinase I: 9% of the activity with ATP Homo sapiens IDP + D-glucose 6-phosphate
-
 ?
additional information not: GTP Homo sapiens ?
-
 ?
UTP + D-glucose hexokinase I: 5% of the activity with ATP Homo sapiens UDP + D-glucose 6-phosphate
-
 ?
UTP + D-glucose MgUTP Homo sapiens UDP + D-glucose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 103000 or 1 * 112000, 2 subtypes of hexokinase type I, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 hexokinase I Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
 5.9