General Stability | Organism |
---|---|
D-glucose, glycerol and thiol-reducing agents stabilize | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | hexokinase C: above 0.2 mM, inhibition partially relieved by ATP concentrations above 1 mM, inhibition is not pH-dependent | Rattus norvegicus | |
D-glucose 6-phosphate | competitive versus ATP; hexokinase C: strong inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
D-glucose | hexokinase C | Rattus norvegicus | |
0.16 | - |
D-glucose | hexokinase B | Rattus norvegicus | |
0.4 | - |
ATP | hexokinase B | Rattus norvegicus | |
0.6 | - |
ATP | hexokinase C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | hexokinases A, B and C, mainly B and C | Rattus norvegicus | 5829 | - |
mitochondrion | hexokinases A and B | Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
hexokinases B and C, gel filtration | Rattus norvegicus |
98000 | - |
1 * 98000, hexokinase B, SDS-PAGE | Rattus norvegicus |
101000 | - |
1 * 101000, hexokinase C, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Rattus norvegicus | glucose metabolism | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley albino rats, hexokinases B and C | - |
Purification (Comment) | Organism |
---|---|
from cytosol, hexokinase B: 539fold, hexokinase C: 235fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Novikoff hepatoma cell | Novikoff ascites-hepatoma cells, hexokinases A, B and C, but not D | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.5 | - |
hexokinase C, at 30°C, pH 7.5 | Rattus norvegicus |
86 | - |
hexokinase B, at 30°C, pH 7.5 | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, in the presence of glucose, glycerol and thiol-reducing agents, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + ATP | hexokinase C | Rattus norvegicus | ADP + D-fructose 6-phosphate | - |
? | |
D-glucose + ATP | - |
Rattus norvegicus | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | glucose metabolism | Rattus norvegicus | D-glucose 6-phosphate + ADP | - |
? | |
D-mannose + ATP | - |
Rattus norvegicus | ADP + D-mannose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 98000, hexokinase B, SDS-PAGE | Rattus norvegicus |
monomer | 1 * 101000, hexokinase C, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
D-glucose 6-phosphate | hexokinase C, versus ATP, at 0.2 mM glucose | Rattus norvegicus |