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Literature summary for 2.7.1.1 extracted from

  • Radojkovic, J.; Ureta, T.
    Hexokinase isoenzymes from the Novikoff hepatoma. Purification, kinetic and structural characterization, with emphasis on hexokinase C (1987), Biochem. J., 242, 895-903.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
D-glucose, glycerol and thiol-reducing agents stabilize Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
D-glucose hexokinase C: above 0.2 mM, inhibition partially relieved by ATP concentrations above 1 mM, inhibition is not pH-dependent Rattus norvegicus
D-glucose 6-phosphate competitive versus ATP; hexokinase C: strong inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
D-glucose hexokinase C Rattus norvegicus
0.16
-
D-glucose hexokinase B Rattus norvegicus
0.4
-
ATP hexokinase B Rattus norvegicus
0.6
-
ATP hexokinase C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol hexokinases A, B and C, mainly B and C Rattus norvegicus 5829
-
mitochondrion hexokinases A and B Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
hexokinases B and C, gel filtration Rattus norvegicus
98000
-
1 * 98000, hexokinase B, SDS-PAGE Rattus norvegicus
101000
-
1 * 101000, hexokinase C, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ATP Rattus norvegicus glucose metabolism D-glucose 6-phosphate + ADP
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Sprague-Dawley albino rats, hexokinases B and C
-

Purification (Commentary)

Purification (Comment) Organism
from cytosol, hexokinase B: 539fold, hexokinase C: 235fold Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
Novikoff hepatoma cell Novikoff ascites-hepatoma cells, hexokinases A, B and C, but not D Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.5
-
hexokinase C, at 30°C, pH 7.5 Rattus norvegicus
86
-
hexokinase B, at 30°C, pH 7.5 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, in the presence of glucose, glycerol and thiol-reducing agents, stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + ATP hexokinase C Rattus norvegicus ADP + D-fructose 6-phosphate
-
?
D-glucose + ATP
-
Rattus norvegicus ADP + D-glucose 6-phosphate
-
?
D-glucose + ATP glucose metabolism Rattus norvegicus D-glucose 6-phosphate + ADP
-
?
D-mannose + ATP
-
Rattus norvegicus ADP + D-mannose 6-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 98000, hexokinase B, SDS-PAGE Rattus norvegicus
monomer 1 * 101000, hexokinase C, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.18
-
D-glucose 6-phosphate hexokinase C, versus ATP, at 0.2 mM glucose Rattus norvegicus