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Literature summary for 2.7.1.1 extracted from
- Purification and crystallization of yeast hexokinase isoenzymes. Characterization of different forms by chromatofocusing (1991), J. Chromatogr., 587, 85-92.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| HXK I and HXK II hexokinase genes are cloned | Saccharomyces cerevisiae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| isoenzymes PI and PII, large crystals in the native and complexed form with glucose | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
1 * 54000, both isoenzymes PI and PII, SDS-PAGE | Saccharomyces cerevisiae |
| 55000 | 60000 | both isoenzymes PI and PII, FPLC gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
overproducing transformed strain WAY.10-1C, isoenzymes PI and PII | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from overproducing strain | Saccharomyces cerevisiae |
| isoenzymes PI and PII | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified isoenzymes PI and PII, 25 mM piperazine-HCl, pH 6.5, 50% glycerol, at least several months, minor loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + ATP | - |
Saccharomyces cerevisiae | ADP + D-fructose 6-phosphate | - |
? |  |
| D-glucose + ATP | - |
Saccharomyces cerevisiae | ADP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 54000, both isoenzymes PI and PII, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
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