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Literature summary for 2.7.1.1 extracted from

  • Hashimoto, M.; Wilson, J.E.
    Kinetic and regulatory properties of HK I+, a modified form of the type I isozyme of mammalian hexokinase in which interactions between the N- and C-terminal halves have been disrupted (2002), Arch. Biochem. Biophys., 399, 109-115.
    View publication on PubMed
Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding HK I is cloned and expressed in M+R42 cells Rattus norvegicus
HK I+, a modified form of HK I with a centrally located polyalanine insert, and D84A mutants of HK I and HK I+ are expressed in M+R42 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D84A HK I mutant with 2fold increased Ki value for the inhibitory 1,5-anhydro-D-glucitol 6-phosphate, mutation diminishes the ability of phosphate to antagonize inhibition, but has no effect on the inhibition by phosphate at higher concentrations Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1,5-Anhydro-D-glucitol 6-phosphate HK I and HK I+, the D84A mutant of HK I has 2fold increased Ki, HK I: low millimolar concentrations of phosphate antagonize inhibition by competing for an anion binding site in the N-terminal half of HK I, HK I+: insert abolishes the antagonism of phosphate Rattus norvegicus
D-glucose 6-phosphate inhibition of HK I, antagonism of inhibition by low millimolar concentrations of phosphate results from competition of this ligands for an anion binding site in the N-terminal half of HK I, mechanism Rattus norvegicus
phosphate at higher concentrations, binds to a lower affinity site in the C-terminal half, HK I and HK I+ Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05 0.053 D-glucose HK I, HKI+ and their D84A mutants Rattus norvegicus
0.45 0.47 ATP HK I and its D84A mutant Rattus norvegicus
1.3 1.4 ATP HK I+ and its D84A mutant Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 HK I Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 HK I+, a modified form of HK I
-
Rattus norvegicus
-
 hexokinase type I
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + ATP
-
 Rattus norvegicus ADP + D-glucose 6-phosphate
-
 ?
additional information ligand binding sites in the N- and C-terminal halves and interactions between these sites Rattus norvegicus ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5 6 1,5-Anhydro-D-glucitol 6-phosphate HK I+ and its D84A mutant Rattus norvegicus
16
-
 1,5-Anhydro-D-glucitol 6-phosphate HK I Rattus norvegicus
32
-
 1,5-Anhydro-D-glucitol 6-phosphate D84A mutant of HK I Rattus norvegicus