Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Sphingopyxis sp. |
Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli ArcticExpress (DE3), His-tag does not affect the enzymatic activity | Sphingopyxis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol | substrate inhibition | Sphingopyxis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol | pH 8.0, 35°C | Sphingopyxis sp. | |
0.49 | - |
pyruvate | pH 8.0, 35°C | Sphingopyxis sp. | |
4.15 | - |
oxaloacetate | pH 8.0, 35°C | Sphingopyxis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48270 | - |
calculated | Sphingopyxis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingopyxis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Sphingopyxis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.2 | - |
pH 8.0, 35°C | Sphingopyxis sp. |
Storage Stability | Organism |
---|---|
-20°C, 25% glycerol, stable for several month | Sphingopyxis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + oxaloacetate | - |
Sphingopyxis sp. | (3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-aspartate | - |
? | |
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + pyruvate | activity observed with glyoxylate and 2-oxobutyrate, no activity detectable with 2-oxogluarate, substrates hydrolyzed fumonisin B2 and B3 also used | Sphingopyxis sp. | (3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminotransferase FumI | - |
Sphingopyxis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Sphingopyxis sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
6 | 45 | - |
Sphingopyxis sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
rapid inactivation at 50°C | Sphingopyxis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.73 | - |
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol | pH 8.0, 35°C | Sphingopyxis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.25 | - |
sharp optimum at pH 8.0 and 8.5 | Sphingopyxis sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.7 | - |
Sphingopyxis sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
activity reduced at pH 6.5 or less (1 h, 35°C), activity reduced at pH 8.0 (1 h) | Sphingopyxis sp. |