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Literature summary for 2.6.1.B21 extracted from

  • Kim, E.M.; Park, J.H.; Kim, B.G.; Seo, J.H.
    Identification of (R)-selective omega-aminotransferases by exploring evolutionary sequence space (2018), Enzyme Microb. Technol., 110, 46-52 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ilvE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Evansella cellulosilytica
gene ilvE1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus thuringiensis
gene pabC, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Saccharopolyspora erythraea
sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Saccharopolyspora erythraea
sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus thuringiensis
sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Evansella cellulosilytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-alpha-methylbenzylamine + pyruvate Saccharopolyspora erythraea
-
acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate Bacillus thuringiensis
-
acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica
-
acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica FERM P-1141
-
acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica ATCC 21833
-
acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica DSM 2522
-
acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica JCM 9156
-
acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica N-4
-
acetophenone + D-alanine
-
r

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis
-
-
-
Bacillus thuringiensis A0A1B1L2T7 serovar berliner ATCC 10792
-
Evansella cellulosilytica
-
-
-
Evansella cellulosilytica E6TUA8
-
-
Evansella cellulosilytica ATCC 21833
-
-
-
Evansella cellulosilytica ATCC 21833 E6TUA8
-
-
Evansella cellulosilytica DSM 2522
-
-
-
Evansella cellulosilytica DSM 2522 E6TUA8
-
-
Evansella cellulosilytica FERM P-1141
-
-
-
Evansella cellulosilytica FERM P-1141 E6TUA8
-
-
Evansella cellulosilytica JCM 9156
-
-
-
Evansella cellulosilytica JCM 9156 E6TUA8
-
-
Evansella cellulosilytica N-4
-
-
-
Evansella cellulosilytica N-4 E6TUA8
-
-
Saccharopolyspora erythraea
-
-
-
Saccharopolyspora erythraea A4FQE5
-
-
Saccharopolyspora erythraea ATCC 11635 A4FQE5
-
-
Saccharopolyspora erythraea DSM 40517 A4FQE5
-
-
Saccharopolyspora erythraea JCM 4748 A4FQE5
-
-
Saccharopolyspora erythraea NBRC 13426 A4FQE5
-
-
Saccharopolyspora erythraea NCIMB 8594 A4FQE5
-
-
Saccharopolyspora erythraea NRRL 2338 A4FQE5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Saccharopolyspora erythraea
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Bacillus thuringiensis
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Evansella cellulosilytica
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Evansella cellulosilytica
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Bacillus thuringiensis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Saccharopolyspora erythraea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000087
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate Bacillus thuringiensis
0.000087
-
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication Bacillus thuringiensis
0.00022
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate Bacillus thuringiensis
0.00022
-
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication Bacillus thuringiensis
0.00027
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate Evansella cellulosilytica
0.00027
-
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication Evansella cellulosilytica
0.00042
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate Evansella cellulosilytica
0.00042
-
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication Evansella cellulosilytica
0.0014
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate Evansella cellulosilytica
0.0014
-
purified recombinant enzyme, substrates L-leucine and 2-oxoglutarate, pH and temperature not specified in the publication Evansella cellulosilytica
0.0015
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate Bacillus thuringiensis
0.0015
-
purified recombinant enzyme, substrates L-leucine and 2-oxoglutarate, pH and temperature not specified in the publication Bacillus thuringiensis
0.003
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates D-alanine and 2-oxoglutarate Saccharopolyspora erythraea
0.003
-
purified recombinant enzyme, substrates D-alanine and 2-oxoglutarate, pH and temperature not specified in the publication Saccharopolyspora erythraea
0.046
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate Saccharopolyspora erythraea
0.046
-
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication Saccharopolyspora erythraea
0.09
-
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate Saccharopolyspora erythraea
0.09
-
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication Saccharopolyspora erythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-alpha-methylbenzylamine + 2-oxoglutarate
-
Saccharopolyspora erythraea acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
-
Bacillus thuringiensis acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
-
Evansella cellulosilytica acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity Evansella cellulosilytica acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity Bacillus thuringiensis acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
-
Evansella cellulosilytica FERM P-1141 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea ATCC 11635 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea NBRC 13426 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
-
Evansella cellulosilytica ATCC 21833 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea JCM 4748 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea NRRL 2338 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea NCIMB 8594 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
-
Evansella cellulosilytica DSM 2522 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
-
Evansella cellulosilytica JCM 9156 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea DSM 40517 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
-
Evansella cellulosilytica N-4 acetophenone + D-glutamate
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Saccharopolyspora erythraea acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Bacillus thuringiensis acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Evansella cellulosilytica acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor Evansella cellulosilytica acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor Bacillus thuringiensis acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Evansella cellulosilytica FERM P-1141 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Saccharopolyspora erythraea ATCC 11635 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Saccharopolyspora erythraea NBRC 13426 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Evansella cellulosilytica ATCC 21833 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Saccharopolyspora erythraea JCM 4748 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Saccharopolyspora erythraea NRRL 2338 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Saccharopolyspora erythraea NCIMB 8594 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Evansella cellulosilytica DSM 2522 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Evansella cellulosilytica JCM 9156 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Saccharopolyspora erythraea DSM 40517 acetophenone + D-alanine
-
r
(R)-alpha-methylbenzylamine + pyruvate
-
Evansella cellulosilytica N-4 acetophenone + D-alanine
-
r
D-alanine + 2-oxoglutarate
-
Saccharopolyspora erythraea pyruvate + D-glutamate
-
r
D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea pyruvate + D-glutamate
-
r
D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea ATCC 11635 pyruvate + D-glutamate
-
r
D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea NBRC 13426 pyruvate + D-glutamate
-
r
D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea JCM 4748 pyruvate + D-glutamate
-
r
D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea NRRL 2338 pyruvate + D-glutamate
-
r
D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea NCIMB 8594 pyruvate + D-glutamate
-
r
D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea DSM 40517 pyruvate + D-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Bacillus thuringiensis 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Bacillus thuringiensis 4-methyl-2-oxopentanoate + D-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica 4-methyl-2-oxopentanoate + D-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica FERM P-1141 4-methyl-2-oxopentanoate + D-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica ATCC 21833 4-methyl-2-oxopentanoate + D-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica DSM 2522 4-methyl-2-oxopentanoate + D-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica JCM 9156 4-methyl-2-oxopentanoate + D-glutamate
-
r
L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica N-4 4-methyl-2-oxopentanoate + D-glutamate
-
r
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bthu also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bthu shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Bacillus thuringiensis ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea ?
-
-
additional information the forward reaction is preferred. Enzyme R-omegaAT_Bthu mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Bacillus thuringiensis ?
-
-
additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica ?
-
-
additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses DAT activity (EC 2.6.1.21). No activity with (S)-alpha-methylbenzylamine and L-leucine Saccharopolyspora erythraea ?
-
-
additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica FERM P-1141 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica FERM P-1141 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea ATCC 11635 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea NBRC 13426 ?
-
-
additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica ATCC 21833 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica ATCC 21833 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea JCM 4748 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea NRRL 2338 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea NCIMB 8594 ?
-
-
additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica DSM 2522 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica DSM 2522 ?
-
-
additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica JCM 9156 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica JCM 9156 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea DSM 40517 ?
-
-
additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica N-4 ?
-
-
additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica N-4 ?
-
-

Synonyms

Synonyms Comment Organism
(R)-selective omega-aminotransferase
-
Saccharopolyspora erythraea
(R)-selective omega-aminotransferase
-
Bacillus thuringiensis
(R)-selective omega-aminotransferase
-
Evansella cellulosilytica
IlvE
-
Evansella cellulosilytica
ilvE1
-
Bacillus thuringiensis
More see also EC 2.6.1.21 Saccharopolyspora erythraea
More see also EC 2.6.1.42 Bacillus thuringiensis
More see also EC 2.6.1.42 Evansella cellulosilytica
PabC
-
Saccharopolyspora erythraea
R-omegaAT
-
Saccharopolyspora erythraea
R-omegaAT
-
Bacillus thuringiensis
R-omegaAT
-
Evansella cellulosilytica
R-omegaAT_Bcel
-
Evansella cellulosilytica
R-omegaAT_Bthu
-
Bacillus thuringiensis
R-omegaAT_Sery
-
Saccharopolyspora erythraea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Evansella cellulosilytica
7
-
assay at Bacillus thuringiensis
7
-
assay at Saccharopolyspora erythraea

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Saccharopolyspora erythraea
pyridoxal 5'-phosphate
-
Bacillus thuringiensis
pyridoxal 5'-phosphate
-
Evansella cellulosilytica
pyridoxal 5'-phosphate PLP, dependent on Evansella cellulosilytica
pyridoxal 5'-phosphate PLP, dependent on Bacillus thuringiensis
pyridoxal 5'-phosphate PLP, dependent on Saccharopolyspora erythraea

General Information

General Information Comment Organism
evolution (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III Saccharopolyspora erythraea
evolution (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III Bacillus thuringiensis
evolution (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III Evansella cellulosilytica
evolution the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G Evansella cellulosilytica
evolution the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G Bacillus thuringiensis
evolution the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G Saccharopolyspora erythraea