Cloned (Comment) | Organism |
---|---|
gene ilvE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Evansella cellulosilytica |
gene ilvE1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus thuringiensis |
gene pabC, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Saccharopolyspora erythraea |
sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Saccharopolyspora erythraea |
sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus thuringiensis |
sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Evansella cellulosilytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-alpha-methylbenzylamine + pyruvate | Saccharopolyspora erythraea | - |
acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | Bacillus thuringiensis | - |
acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | Evansella cellulosilytica | - |
acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | Evansella cellulosilytica FERM P-1141 | - |
acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | Evansella cellulosilytica ATCC 21833 | - |
acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | Evansella cellulosilytica DSM 2522 | - |
acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | Evansella cellulosilytica JCM 9156 | - |
acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | Evansella cellulosilytica N-4 | - |
acetophenone + D-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Bacillus thuringiensis | A0A1B1L2T7 | serovar berliner ATCC 10792 | - |
Evansella cellulosilytica | - |
- |
- |
Evansella cellulosilytica | E6TUA8 | - |
- |
Evansella cellulosilytica ATCC 21833 | - |
- |
- |
Evansella cellulosilytica ATCC 21833 | E6TUA8 | - |
- |
Evansella cellulosilytica DSM 2522 | - |
- |
- |
Evansella cellulosilytica DSM 2522 | E6TUA8 | - |
- |
Evansella cellulosilytica FERM P-1141 | - |
- |
- |
Evansella cellulosilytica FERM P-1141 | E6TUA8 | - |
- |
Evansella cellulosilytica JCM 9156 | - |
- |
- |
Evansella cellulosilytica JCM 9156 | E6TUA8 | - |
- |
Evansella cellulosilytica N-4 | - |
- |
- |
Evansella cellulosilytica N-4 | E6TUA8 | - |
- |
Saccharopolyspora erythraea | - |
- |
- |
Saccharopolyspora erythraea | A4FQE5 | - |
- |
Saccharopolyspora erythraea ATCC 11635 | A4FQE5 | - |
- |
Saccharopolyspora erythraea DSM 40517 | A4FQE5 | - |
- |
Saccharopolyspora erythraea JCM 4748 | A4FQE5 | - |
- |
Saccharopolyspora erythraea NBRC 13426 | A4FQE5 | - |
- |
Saccharopolyspora erythraea NCIMB 8594 | A4FQE5 | - |
- |
Saccharopolyspora erythraea NRRL 2338 | A4FQE5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Saccharopolyspora erythraea |
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Bacillus thuringiensis |
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Evansella cellulosilytica |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Evansella cellulosilytica |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Bacillus thuringiensis |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | Saccharopolyspora erythraea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000087 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate | Bacillus thuringiensis |
0.000087 | - |
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication | Bacillus thuringiensis |
0.00022 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate | Bacillus thuringiensis |
0.00022 | - |
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication | Bacillus thuringiensis |
0.00027 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate | Evansella cellulosilytica |
0.00027 | - |
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication | Evansella cellulosilytica |
0.00042 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate | Evansella cellulosilytica |
0.00042 | - |
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication | Evansella cellulosilytica |
0.0014 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate | Evansella cellulosilytica |
0.0014 | - |
purified recombinant enzyme, substrates L-leucine and 2-oxoglutarate, pH and temperature not specified in the publication | Evansella cellulosilytica |
0.0015 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate | Bacillus thuringiensis |
0.0015 | - |
purified recombinant enzyme, substrates L-leucine and 2-oxoglutarate, pH and temperature not specified in the publication | Bacillus thuringiensis |
0.003 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates D-alanine and 2-oxoglutarate | Saccharopolyspora erythraea |
0.003 | - |
purified recombinant enzyme, substrates D-alanine and 2-oxoglutarate, pH and temperature not specified in the publication | Saccharopolyspora erythraea |
0.046 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate | Saccharopolyspora erythraea |
0.046 | - |
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication | Saccharopolyspora erythraea |
0.09 | - |
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate | Saccharopolyspora erythraea |
0.09 | - |
purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication | Saccharopolyspora erythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-alpha-methylbenzylamine + 2-oxoglutarate | - |
Saccharopolyspora erythraea | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | - |
Bacillus thuringiensis | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | - |
Evansella cellulosilytica | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | very low activity | Evansella cellulosilytica | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | very low activity | Bacillus thuringiensis | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | low activity | Saccharopolyspora erythraea | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | - |
Evansella cellulosilytica FERM P-1141 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | low activity | Saccharopolyspora erythraea ATCC 11635 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | low activity | Saccharopolyspora erythraea NBRC 13426 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | - |
Evansella cellulosilytica ATCC 21833 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | low activity | Saccharopolyspora erythraea JCM 4748 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | low activity | Saccharopolyspora erythraea NRRL 2338 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | low activity | Saccharopolyspora erythraea NCIMB 8594 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | - |
Evansella cellulosilytica DSM 2522 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | - |
Evansella cellulosilytica JCM 9156 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | low activity | Saccharopolyspora erythraea DSM 40517 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + 2-oxoglutarate | - |
Evansella cellulosilytica N-4 | acetophenone + D-glutamate | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Saccharopolyspora erythraea | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Bacillus thuringiensis | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Evansella cellulosilytica | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | the enzyme prefers pyruvate as the amino acceptor | Evansella cellulosilytica | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | the enzyme prefers pyruvate as the amino acceptor | Bacillus thuringiensis | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Evansella cellulosilytica FERM P-1141 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Saccharopolyspora erythraea ATCC 11635 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Saccharopolyspora erythraea NBRC 13426 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Evansella cellulosilytica ATCC 21833 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Saccharopolyspora erythraea JCM 4748 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Saccharopolyspora erythraea NRRL 2338 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Saccharopolyspora erythraea NCIMB 8594 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Evansella cellulosilytica DSM 2522 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Evansella cellulosilytica JCM 9156 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Saccharopolyspora erythraea DSM 40517 | acetophenone + D-alanine | - |
r | |
(R)-alpha-methylbenzylamine + pyruvate | - |
Evansella cellulosilytica N-4 | acetophenone + D-alanine | - |
r | |
D-alanine + 2-oxoglutarate | - |
Saccharopolyspora erythraea | pyruvate + D-glutamate | - |
r | |
D-alanine + 2-oxoglutarate | very low activity, reaction of EC 2.6.1.21 | Saccharopolyspora erythraea | pyruvate + D-glutamate | - |
r | |
D-alanine + 2-oxoglutarate | very low activity, reaction of EC 2.6.1.21 | Saccharopolyspora erythraea ATCC 11635 | pyruvate + D-glutamate | - |
r | |
D-alanine + 2-oxoglutarate | very low activity, reaction of EC 2.6.1.21 | Saccharopolyspora erythraea NBRC 13426 | pyruvate + D-glutamate | - |
r | |
D-alanine + 2-oxoglutarate | very low activity, reaction of EC 2.6.1.21 | Saccharopolyspora erythraea JCM 4748 | pyruvate + D-glutamate | - |
r | |
D-alanine + 2-oxoglutarate | very low activity, reaction of EC 2.6.1.21 | Saccharopolyspora erythraea NRRL 2338 | pyruvate + D-glutamate | - |
r | |
D-alanine + 2-oxoglutarate | very low activity, reaction of EC 2.6.1.21 | Saccharopolyspora erythraea NCIMB 8594 | pyruvate + D-glutamate | - |
r | |
D-alanine + 2-oxoglutarate | very low activity, reaction of EC 2.6.1.21 | Saccharopolyspora erythraea DSM 40517 | pyruvate + D-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Evansella cellulosilytica | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Bacillus thuringiensis | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Bacillus thuringiensis | 4-methyl-2-oxopentanoate + D-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Evansella cellulosilytica | 4-methyl-2-oxopentanoate + D-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Evansella cellulosilytica FERM P-1141 | 4-methyl-2-oxopentanoate + D-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Evansella cellulosilytica ATCC 21833 | 4-methyl-2-oxopentanoate + D-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Evansella cellulosilytica DSM 2522 | 4-methyl-2-oxopentanoate + D-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Evansella cellulosilytica JCM 9156 | 4-methyl-2-oxopentanoate + D-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | reaction of EC 2.6.1.42 | Evansella cellulosilytica N-4 | 4-methyl-2-oxopentanoate + D-glutamate | - |
r | |
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity | Evansella cellulosilytica | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Bthu also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bthu shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity | Bacillus thuringiensis | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine | Saccharopolyspora erythraea | ? | - |
- |
|
additional information | the forward reaction is preferred. Enzyme R-omegaAT_Bthu mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine | Bacillus thuringiensis | ? | - |
- |
|
additional information | the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine | Evansella cellulosilytica | ? | - |
- |
|
additional information | the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses DAT activity (EC 2.6.1.21). No activity with (S)-alpha-methylbenzylamine and L-leucine | Saccharopolyspora erythraea | ? | - |
- |
|
additional information | the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine | Evansella cellulosilytica FERM P-1141 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity | Evansella cellulosilytica FERM P-1141 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine | Saccharopolyspora erythraea ATCC 11635 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine | Saccharopolyspora erythraea NBRC 13426 | ? | - |
- |
|
additional information | the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine | Evansella cellulosilytica ATCC 21833 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity | Evansella cellulosilytica ATCC 21833 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine | Saccharopolyspora erythraea JCM 4748 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine | Saccharopolyspora erythraea NRRL 2338 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine | Saccharopolyspora erythraea NCIMB 8594 | ? | - |
- |
|
additional information | the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine | Evansella cellulosilytica DSM 2522 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity | Evansella cellulosilytica DSM 2522 | ? | - |
- |
|
additional information | the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine | Evansella cellulosilytica JCM 9156 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity | Evansella cellulosilytica JCM 9156 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine | Saccharopolyspora erythraea DSM 40517 | ? | - |
- |
|
additional information | the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine | Evansella cellulosilytica N-4 | ? | - |
- |
|
additional information | besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity | Evansella cellulosilytica N-4 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
(R)-selective omega-aminotransferase | - |
Saccharopolyspora erythraea |
(R)-selective omega-aminotransferase | - |
Bacillus thuringiensis |
(R)-selective omega-aminotransferase | - |
Evansella cellulosilytica |
IlvE | - |
Evansella cellulosilytica |
ilvE1 | - |
Bacillus thuringiensis |
More | see also EC 2.6.1.21 | Saccharopolyspora erythraea |
More | see also EC 2.6.1.42 | Bacillus thuringiensis |
More | see also EC 2.6.1.42 | Evansella cellulosilytica |
PabC | - |
Saccharopolyspora erythraea |
R-omegaAT | - |
Saccharopolyspora erythraea |
R-omegaAT | - |
Bacillus thuringiensis |
R-omegaAT | - |
Evansella cellulosilytica |
R-omegaAT_Bcel | - |
Evansella cellulosilytica |
R-omegaAT_Bthu | - |
Bacillus thuringiensis |
R-omegaAT_Sery | - |
Saccharopolyspora erythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Evansella cellulosilytica |
7 | - |
assay at | Bacillus thuringiensis |
7 | - |
assay at | Saccharopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Saccharopolyspora erythraea | |
pyridoxal 5'-phosphate | - |
Bacillus thuringiensis | |
pyridoxal 5'-phosphate | - |
Evansella cellulosilytica | |
pyridoxal 5'-phosphate | PLP, dependent on | Evansella cellulosilytica | |
pyridoxal 5'-phosphate | PLP, dependent on | Bacillus thuringiensis | |
pyridoxal 5'-phosphate | PLP, dependent on | Saccharopolyspora erythraea |
General Information | Comment | Organism |
---|---|---|
evolution | (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III | Saccharopolyspora erythraea |
evolution | (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III | Bacillus thuringiensis |
evolution | (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III | Evansella cellulosilytica |
evolution | the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G | Evansella cellulosilytica |
evolution | the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G | Bacillus thuringiensis |
evolution | the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G | Saccharopolyspora erythraea |