Cloned (Comment) | Organism |
---|---|
expression of GST-TAA1 in Escherichia coli strain BL21 Star (DE3) pLysS | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-kynurenine | an alternate substrate, competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions. L-kynurenine application represses activity and nuclear accumulation of the EIN3 transcription factor in roots. L-kynurenine effectively and selectively binds to the substrate pocket of TAA1/TAR proteins but not to those of other families of aminotransferases, computational docking and molecular modeling, overview | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + pyruvate | Arabidopsis thaliana | - |
indole-3-pyruvate + L-alanine | - |
? | |
L-tryptophan + pyruvate | Arabidopsis thaliana Col-0 | - |
indole-3-pyruvate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S7N2 | - |
- |
Arabidopsis thaliana Col-0 | Q9S7N2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + pyruvate | - |
Arabidopsis thaliana | indole-3-pyruvate + L-alanine | - |
? | |
L-tryptophan + pyruvate | - |
Arabidopsis thaliana Col-0 | indole-3-pyruvate + L-alanine | - |
? | |
additional information | L-kynurenine is an alternate substrate competing with L-tryptophan | Arabidopsis thaliana | ? | - |
? | |
additional information | L-kynurenine is an alternate substrate competing with L-tryptophan | Arabidopsis thaliana Col-0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TAA1 | - |
Arabidopsis thaliana |
tryptophan aminotransferase of Arabidopsis 1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01152 | - |
L-kynurenine | inhibition of TAA1, competitive versus L-Tryp, pH not specificied in the publication, 55°C | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | L-kynurenine, being an alternate substrate, competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions | Arabidopsis thaliana |
metabolism | tryptophan aminotransferase of Arabidopsis 1 and tryptopan aminotransferase related, i.e. TAA1 and TAR, are the key enzymes in the indole-3-pyruvic acid pathway of auxin biosynthesis | Arabidopsis thaliana |