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Literature summary for 2.6.1.9 extracted from

  • Ames, B.N.; Horecker, B.L.
    Biosynthesis of histidine: imidazoleacetol phosphate transaminase (1956), J. Biol. Chem., 220, 113-128.
    View publication on PubMed
Search for more literature for 2.6.1.9

Activating Compound

Activating Compound Comment Organism Structure
omega-Methylpyridoxal phosphate can replace pyridoxal phosphate, Km: 0.004 mM Neurospora sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.004 M, 80% inhibition Neurospora sp.
Cu2+ 0.004 M, 80% inhibition Neurospora sp.
hydroxylamine 0.004 M, 80% inhibition Neurospora sp.
iodoacetate 0.004 M, 95% inhibition Neurospora sp.
Semicarbazide 0.004 M, 80% inhibition Neurospora sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 L-histidinol phosphate pH 8.1, 37°C Neurospora sp.
1.2
-
 2-oxoglutarate pH 8.1, 37°C Neurospora sp.

Organism

Organism UniProt Comment Textmining
Neurospora sp.
-
 C141-T1710, C84, C141
-

Purification (Commentary)

Purification (Comment) Organism
C141-T1710, C141 Neurospora sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.3
-
-
 Neurospora sp.

Storage Stability

Storage Stability Organism
-20°C, stable for several months Neurospora sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-2-aminoadipate 40% of the activity with L-glutamate Neurospora sp. L-histidinol phosphate + 2-oxoadipate
-
 r
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-arginine 33% of the activity with L-glutamate Neurospora sp. L-histidinol phosphate + 2-oxo-5-guanidinopentanoate
-
 r
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamine 4% of the activity with L-glutamate Neurospora sp. L-histidinol phosphate + 4-carbamoyl-2-oxobutanoate
-
 r
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-histidine 30% of the activity with L-glutamate Neurospora sp. L-histidinol phosphate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
-
 r
L-histidinol phosphate + 2-oxo-5-guanidinopentanoate
-
 Neurospora sp. 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-arginine
-
 r
L-histidinol phosphate + 2-oxoadipate 70% of the activity with 2-oxoglutarate Neurospora sp. 3-(imidazol-4-yl)-2-oxopropyl phosphate + 2-aminoadipate
-
 r
L-histidinol phosphate + 2-oxoglutarate
-
 Neurospora sp. 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu i.e. imidazoleacetol phosphate + L-Glu r
additional information no substrate: oxaloacetate, pyruvate Neurospora sp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Neurospora sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 diphosphate buffer Neurospora sp.
8.4
-
 diethanolamine buffer Neurospora sp.
8.5
-
 triethanolamine buffer Neurospora sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 pH 7.5: about 85% of activity maximum, pH 9.0: about 50% of activity maximum, diphosphate buffer Neurospora sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km: 0.0012 mM Neurospora sp.
pyridoxal 5'-phosphate a pyridoxal phosphate protein Neurospora sp.