BRENDA - Enzyme Database show
show all sequences of 2.6.1.88

Tyrosine aminotransferase catalyzes the final step of methionine recycling in Klebsiella pneumonia

Heilbronn, J.; Wilson, J.; Berger, B.J.; J. Bacteriol. 181, 1739-1747 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Klebsiella pneumoniae
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-nitrotyrosine
approximately 20% inhibition at 1 mM
Klebsiella pneumoniae
Canaline
approximately 40% inhibition at 1 mM
Klebsiella pneumoniae
carboxymethoxylamine
complete inhibition at 1 mM
Klebsiella pneumoniae
malate
approximately 20% inhibition at 1 mM
Klebsiella pneumoniae
additional information
serine-O-sulfate and nitrophenylalanine have no inhibitory activity at 0.1 or 1 mM
Klebsiella pneumoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.42
-
L-tryptophan
recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
2.01
-
L-phenylalanine
recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
2.01
-
L-tyrosine
recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
2.46
-
2-oxo-4-methylthiobutanoate
recombinant enzyme, with L-tyrosine as cosubstrate, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
11.93
-
L-glutamate
recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Klebsiella pneumoniae
90000
-
gel filtration
Klebsiella pneumoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae ATCC 13883
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE-Sepharose column chromatography, Red-120 Sepharose column chromatography, Mono-Q column chromatography, S200-Sephacryl gel filtration, and phenylethyl column chromatography
Klebsiella pneumoniae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00314
-
crude recombinant enzyme, after incubation with 2 mM each amino acid (ADEFGHIKLNQRSTWY) and 1 mM 2-oxo-4-methylthiobutanoate in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
0.7227
-
recombinant enzyme after 722.7fold purification, after incubation with 2 mM each amino acid (ADEFGHIKLNQRSTWY) and 1 mM 2-oxo-4-methylthiobutanoate in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxo-4-methylthiobutanoate + an L-amino acid
a wide range of L-amino acids are effective donors, with Asp, Glu, Phe, His, Ile, Leu, Asn, Gln, Trp, and Tyr all producing more than 0.5 nM of L-methionine/min/mg of protein. Glu, Phe, Trp, and Tyr are the preferred amino donors, catalyzing the formation of above 1100 nM L-methionine/min/mg of protein
715336
Klebsiella pneumoniae
L-methionine + a 2-oxo acid
-
-
-
?
2-oxo-4-methylthiobutanoate + an L-amino acid
a wide range of L-amino acids are effective donors, with Asp, Glu, Phe, His, Ile, Leu, Asn, Gln, Trp, and Tyr all producing more than 0.5 nM of L-methionine/min/mg of protein. Glu, Phe, Trp, and Tyr are the preferred amino donors, catalyzing the formation of above 1100 nM L-methionine/min/mg of protein
715336
Klebsiella pneumoniae ATCC 13883
L-methionine + a 2-oxo acid
-
-
-
?
2-oxo-4-methylthiobutanoate + L-glutamate
while L-glutamate is capable of producing the highest maximum initial velocity for L-methionine production from 2-oxo-4-methylthiobutanoate, it is 5-10fold poorer in terms of substrate specificity when compared with L-tyrosine, L-tryptophan, and L-phenylalanine
715336
Klebsiella pneumoniae
L-methionine + 2-oxoglutarate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-glutamate
while L-glutamate is capable of producing the highest maximum initial velocity for L-methionine production from 2-oxo-4-methylthiobutanoate, it is 5-10fold poorer in terms of substrate specificity when compared with L-tyrosine, L-tryptophan, and L-phenylalanine
715336
Klebsiella pneumoniae ATCC 13883
L-methionine + 2-oxoglutarate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-phenylalanine
-
715336
Klebsiella pneumoniae
L-methionine + 2-oxo-3-phenylpropanoate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-phenylalanine
-
715336
Klebsiella pneumoniae ATCC 13883
L-methionine + 2-oxo-3-phenylpropanoate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-tryptophan
-
715336
Klebsiella pneumoniae
L-methionine + 3-(1H-indol-3-yl)-2-oxopropanoate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-tryptophan
-
715336
Klebsiella pneumoniae ATCC 13883
L-methionine + 3-(1H-indol-3-yl)-2-oxopropanoate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-tyrosine
-
715336
Klebsiella pneumoniae
L-methionine + 4-hydroxyhenylpyruvate
-
-
-
r
L-methionine + 2-oxoglutarate
-
715336
Klebsiella pneumoniae
2-oxo-4-methylthiobutanoate + L-glutamate
-
-
-
r
additional information
the purified enzyme is unable to effectively utilize systems containing L-glutamate and oxaloacetate, L-glutamate and pyruvate, or L-alanine and 2-oxoglutarate
715336
Klebsiella pneumoniae
?
-
-
-
-
additional information
the purified enzyme is unable to effectively utilize systems containing L-glutamate and oxaloacetate, L-glutamate and pyruvate, or L-alanine and 2-oxoglutarate
715336
Klebsiella pneumoniae ATCC 13883
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 45000, SDS-PAGE
Klebsiella pneumoniae
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Klebsiella pneumoniae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Klebsiella pneumoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-nitrotyrosine
approximately 20% inhibition at 1 mM
Klebsiella pneumoniae
Canaline
approximately 40% inhibition at 1 mM
Klebsiella pneumoniae
carboxymethoxylamine
complete inhibition at 1 mM
Klebsiella pneumoniae
malate
approximately 20% inhibition at 1 mM
Klebsiella pneumoniae
additional information
serine-O-sulfate and nitrophenylalanine have no inhibitory activity at 0.1 or 1 mM
Klebsiella pneumoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.42
-
L-tryptophan
recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
2.01
-
L-phenylalanine
recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
2.01
-
L-tyrosine
recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
2.46
-
2-oxo-4-methylthiobutanoate
recombinant enzyme, with L-tyrosine as cosubstrate, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
11.93
-
L-glutamate
recombinant enzyme, in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Klebsiella pneumoniae
90000
-
gel filtration
Klebsiella pneumoniae
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sepharose column chromatography, Red-120 Sepharose column chromatography, Mono-Q column chromatography, S200-Sephacryl gel filtration, and phenylethyl column chromatography
Klebsiella pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00314
-
crude recombinant enzyme, after incubation with 2 mM each amino acid (ADEFGHIKLNQRSTWY) and 1 mM 2-oxo-4-methylthiobutanoate in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
0.7227
-
recombinant enzyme after 722.7fold purification, after incubation with 2 mM each amino acid (ADEFGHIKLNQRSTWY) and 1 mM 2-oxo-4-methylthiobutanoate in 10 mM phosphate buffer (pH 7.4), 50 mM pyridoxal 5'-phosphate, at 37°C
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxo-4-methylthiobutanoate + an L-amino acid
a wide range of L-amino acids are effective donors, with Asp, Glu, Phe, His, Ile, Leu, Asn, Gln, Trp, and Tyr all producing more than 0.5 nM of L-methionine/min/mg of protein. Glu, Phe, Trp, and Tyr are the preferred amino donors, catalyzing the formation of above 1100 nM L-methionine/min/mg of protein
715336
Klebsiella pneumoniae
L-methionine + a 2-oxo acid
-
-
-
?
2-oxo-4-methylthiobutanoate + an L-amino acid
a wide range of L-amino acids are effective donors, with Asp, Glu, Phe, His, Ile, Leu, Asn, Gln, Trp, and Tyr all producing more than 0.5 nM of L-methionine/min/mg of protein. Glu, Phe, Trp, and Tyr are the preferred amino donors, catalyzing the formation of above 1100 nM L-methionine/min/mg of protein
715336
Klebsiella pneumoniae ATCC 13883
L-methionine + a 2-oxo acid
-
-
-
?
2-oxo-4-methylthiobutanoate + L-glutamate
while L-glutamate is capable of producing the highest maximum initial velocity for L-methionine production from 2-oxo-4-methylthiobutanoate, it is 5-10fold poorer in terms of substrate specificity when compared with L-tyrosine, L-tryptophan, and L-phenylalanine
715336
Klebsiella pneumoniae
L-methionine + 2-oxoglutarate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-glutamate
while L-glutamate is capable of producing the highest maximum initial velocity for L-methionine production from 2-oxo-4-methylthiobutanoate, it is 5-10fold poorer in terms of substrate specificity when compared with L-tyrosine, L-tryptophan, and L-phenylalanine
715336
Klebsiella pneumoniae ATCC 13883
L-methionine + 2-oxoglutarate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-phenylalanine
-
715336
Klebsiella pneumoniae
L-methionine + 2-oxo-3-phenylpropanoate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-phenylalanine
-
715336
Klebsiella pneumoniae ATCC 13883
L-methionine + 2-oxo-3-phenylpropanoate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-tryptophan
-
715336
Klebsiella pneumoniae
L-methionine + 3-(1H-indol-3-yl)-2-oxopropanoate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-tryptophan
-
715336
Klebsiella pneumoniae ATCC 13883
L-methionine + 3-(1H-indol-3-yl)-2-oxopropanoate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-tyrosine
-
715336
Klebsiella pneumoniae
L-methionine + 4-hydroxyhenylpyruvate
-
-
-
r
L-methionine + 2-oxoglutarate
-
715336
Klebsiella pneumoniae
2-oxo-4-methylthiobutanoate + L-glutamate
-
-
-
r
additional information
the purified enzyme is unable to effectively utilize systems containing L-glutamate and oxaloacetate, L-glutamate and pyruvate, or L-alanine and 2-oxoglutarate
715336
Klebsiella pneumoniae
?
-
-
-
-
additional information
the purified enzyme is unable to effectively utilize systems containing L-glutamate and oxaloacetate, L-glutamate and pyruvate, or L-alanine and 2-oxoglutarate
715336
Klebsiella pneumoniae ATCC 13883
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 45000, SDS-PAGE
Klebsiella pneumoniae
Other publictions for EC 2.6.1.88
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
676421
Schuster
Branched-chain aminotransferas ...
Arabidopsis thaliana
Plant Cell
18
2664-2679
2006
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
677670
Martinez-Cuesta
YtjE from Lactococcus lactis I ...
no activity in Lactococcus lactis, no activity in Lactococcus lactis IL1403
Appl. Environ. Microbiol.
72
4878-4884
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661400
Venos
Branched-chain amino acid amin ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
BMC Microbiol.
4
39
2004
-
-
1
-
-
-
12
6
-
-
-
-
-
152
-
-
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
1
8
-
-
-
-
1
1
-
-
-
-
12
8
6
-
-
-
-
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715008
Dolzan
Crystal structure and reactivi ...
Escherichia coli
FEBS Lett.
571
141-146
2004
-
-
1
1
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715336
Heilbronn
Tyrosine aminotransferase cata ...
Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 13883
J. Bacteriol.
181
1739-1747
1999
-
-
1
-
-
-
5
5
-
-
2
-
-
3
-
-
1
-
-
-
2
-
12
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
5
-
5
-
-
2
-
-
-
-
1
-
-
2
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-