BRENDA - Enzyme Database show
show all sequences of 2.6.1.88

Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis

Venos, E.S.; Knodel, M.H.; Radford, C.L.; Berger, B.J.; BMC Microbiol. 4, 39 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells
Mycobacterium tuberculosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
Canaline
about 50% residual activity at 1 mM
Mycobacterium tuberculosis
carboxymethoxylamine
mixed-type inhibition, about 7% residual activity at 1 mM
Mycobacterium tuberculosis
hydroxylamine-O-sulfonic acid
about 10% residual activity at 1 mM
Mycobacterium tuberculosis
additional information
not inhibited by O-trimethylsilylhydroxylamine
Mycobacterium tuberculosis
O-allylhydroxylamine
mixed-type inhibition, about 10% residual activity at 1 mM
Mycobacterium tuberculosis
O-Benzylhydroxylamine
mixed-type inhibition, about 10% residual activity at 1 mM
Mycobacterium tuberculosis
O-ethylhydroxylamine
about 20% residual activity at 1 mM
Mycobacterium tuberculosis
O-nitrobenzylhydroxylamine
about 30% residual activity at 1 mM
Mycobacterium tuberculosis
O-pentafluorobenzylhydroxylamine
about 30% residual activity at 1 mM
Mycobacterium tuberculosis
O-tert-butyldimethylsilylhydroxylamine
about 10% residual activity at 1 mM
Mycobacterium tuberculosis
O-tert-butylhydroxylamine
mixed-type inhibition, about 7% residual activity at 1 mM
Mycobacterium tuberculosis
O-tetrahydropyranylhydroxylamine
about 20% residual activity at 1 mM
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.77
-
L-valine
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
2.5
-
L-leucine
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
2.85
-
L-isoleucine
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
4.2
-
2-oxo-4-methylthiobutanoate
apparent Km value, with L-leucine as cosubstrate, at pH 7.4, 37C
Mycobacterium tuberculosis
7.44
-
L-phenylalanine
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
9.53
-
L-glutamate
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Purification (Commentary)
Commentary
Organism
Ni2+ affinity column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxo-4-methylthiobutanoate + an L-amino acid
isoleucine, leucine, and valine are the most effective substrates while glutamate and phenylalanine are also active as amino donors. Tyrosine and tryptophan have a much lesser ability to transaminate 2-oxo-4-methylthiobutanoate and all other L-amino acids are inactive
661400
Mycobacterium tuberculosis
L-methionine + a 2-oxo acid
-
-
-
?
2-oxo-4-methylthiobutanoate + L-glutamate
-
661400
Mycobacterium tuberculosis
L-methionine + ?
-
-
-
?
2-oxo-4-methylthiobutanoate + L-isoleucine
-
661400
Mycobacterium tuberculosis
L-methionine + 2-oxo-methylvalerate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-leucine
-
661400
Mycobacterium tuberculosis
L-methionine + 2-oxo-isocaproate
-
-
-
r
2-oxo-4-methylthiobutanoate + L-phenylalanine
-
661400
Mycobacterium tuberculosis
L-methionine + ?
-
-
-
?
2-oxo-4-methylthiobutanoate + L-valine
-
661400
Mycobacterium tuberculosis
L-methionine + 2-oxo-isovalerate
-
-
-
?
L-methionine + 2-oxo-isocaproate
-
661400
Mycobacterium tuberculosis
2-oxo-4-methylthiobutanoate + L-leucine
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Mycobacterium tuberculosis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0082
-
O-Benzylhydroxylamine
competitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.01102
-
O-tert-butylhydroxylamine
competitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.02097
-
carboxymethoxylamine
competitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.02161
-
O-allylhydroxylamine
competitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.08408
-
O-Benzylhydroxylamine
uncompetitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.0856
-
O-tert-butylhydroxylamine
uncompetitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.1424
-
carboxymethoxylamine
uncompetitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.2
-
O-allylhydroxylamine
Ki above 0.2 mM, uncompetitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells
Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Mycobacterium tuberculosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Canaline
about 50% residual activity at 1 mM
Mycobacterium tuberculosis
carboxymethoxylamine
mixed-type inhibition, about 7% residual activity at 1 mM
Mycobacterium tuberculosis
hydroxylamine-O-sulfonic acid
about 10% residual activity at 1 mM
Mycobacterium tuberculosis
additional information
not inhibited by O-trimethylsilylhydroxylamine
Mycobacterium tuberculosis
O-allylhydroxylamine
mixed-type inhibition, about 10% residual activity at 1 mM
Mycobacterium tuberculosis
O-Benzylhydroxylamine
mixed-type inhibition, about 10% residual activity at 1 mM
Mycobacterium tuberculosis
O-ethylhydroxylamine
about 20% residual activity at 1 mM
Mycobacterium tuberculosis
O-nitrobenzylhydroxylamine
about 30% residual activity at 1 mM
Mycobacterium tuberculosis
O-pentafluorobenzylhydroxylamine
about 30% residual activity at 1 mM
Mycobacterium tuberculosis
O-tert-butyldimethylsilylhydroxylamine
about 10% residual activity at 1 mM
Mycobacterium tuberculosis
O-tert-butylhydroxylamine
mixed-type inhibition, about 7% residual activity at 1 mM
Mycobacterium tuberculosis
O-tetrahydropyranylhydroxylamine
about 20% residual activity at 1 mM
Mycobacterium tuberculosis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0082
-
O-Benzylhydroxylamine
competitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.01102
-
O-tert-butylhydroxylamine
competitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.02097
-
carboxymethoxylamine
competitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.02161
-
O-allylhydroxylamine
competitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.08408
-
O-Benzylhydroxylamine
uncompetitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.0856
-
O-tert-butylhydroxylamine
uncompetitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.1424
-
carboxymethoxylamine
uncompetitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
0.2
-
O-allylhydroxylamine
Ki above 0.2 mM, uncompetitive inhibition, at pH 7.4, 37C
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.77
-
L-valine
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
2.5
-
L-leucine
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
2.85
-
L-isoleucine
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
4.2
-
2-oxo-4-methylthiobutanoate
apparent Km value, with L-leucine as cosubstrate, at pH 7.4, 37C
Mycobacterium tuberculosis
7.44
-
L-phenylalanine
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
9.53
-
L-glutamate
apparent Km value, at pH 7.4, 37C
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+ affinity column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxo-4-methylthiobutanoate + an L-amino acid
isoleucine, leucine, and valine are the most effective substrates while glutamate and phenylalanine are also active as amino donors. Tyrosine and tryptophan have a much lesser ability to transaminate 2-oxo-4-methylthiobutanoate and all other L-amino acids are inactive
661400
Mycobacterium tuberculosis
L-methionine + a 2-oxo acid
-
-
-
?
2-oxo-4-methylthiobutanoate + L-glutamate
-
661400
Mycobacterium tuberculosis
L-methionine + ?
-
-
-
?
2-oxo-4-methylthiobutanoate + L-isoleucine
-
661400
Mycobacterium tuberculosis
L-methionine + 2-oxo-methylvalerate
-
-
-
?
2-oxo-4-methylthiobutanoate + L-leucine
-
661400
Mycobacterium tuberculosis
L-methionine + 2-oxo-isocaproate
-
-
-
r
2-oxo-4-methylthiobutanoate + L-phenylalanine
-
661400
Mycobacterium tuberculosis
L-methionine + ?
-
-
-
?
2-oxo-4-methylthiobutanoate + L-valine
-
661400
Mycobacterium tuberculosis
L-methionine + 2-oxo-isovalerate
-
-
-
?
L-methionine + 2-oxo-isocaproate
-
661400
Mycobacterium tuberculosis
2-oxo-4-methylthiobutanoate + L-leucine
-
-
-
r
Other publictions for EC 2.6.1.88
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
676421
Schuster
Branched-chain aminotransferas ...
Arabidopsis thaliana
Plant Cell
18
2664-2679
2006
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
677670
Martinez-Cuesta
YtjE from Lactococcus lactis I ...
no activity in Lactococcus lactis, no activity in Lactococcus lactis IL1403
Appl. Environ. Microbiol.
72
4878-4884
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661400
Venos
Branched-chain amino acid amin ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
BMC Microbiol.
4
39
2004
-
-
1
-
-
-
12
6
-
-
-
-
-
152
-
-
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
1
8
-
-
-
-
1
1
-
-
-
-
12
8
6
-
-
-
-
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715008
Dolzan
Crystal structure and reactivi ...
Escherichia coli
FEBS Lett.
571
141-146
2004
-
-
1
1
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715336
Heilbronn
Tyrosine aminotransferase cata ...
Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 13883
J. Bacteriol.
181
1739-1747
1999
-
-
1
-
-
-
5
5
-
-
2
-
-
3
-
-
1
-
-
-
2
-
12
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
5
-
5
-
-
2
-
-
-
-
1
-
-
2
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-