BRENDA - Enzyme Database
show all sequences of 2.6.1.86

Ligand binding induces an ammonia channel in 2-amino-2-desoxyisochorismate (ADIC) synthase PhzE

Li, Q.A.; Mavrodi, D.V.; Thomashow, L.S.; Roessle, M.; Blankenfeldt, W.; J. Biol. Chem. 286, 18213-18221 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
His-tagged protein expressed in Escherichia coli BL21(DE3)
Burkholderia lata
Crystallization (Commentary)
Crystallization (Commentary)
Organism
hanging-drop vapor diffusion method, PEG 3350
Burkholderia lata
Inhibitors
Inhibitors
Commentary
Organism
Structure
Mn2+
reduced activity
Burkholderia lata
Ni2+
reduced activity
Burkholderia lata
Zn2+
reduced activity
Burkholderia lata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0024
-
chorismate
pH 7.0, 25°C
Burkholderia lata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Burkholderia lata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals
Burkholderia lata
Organism
Organism
UniProt
Commentary
Textmining
Burkholderia lata
-
-
-
Burkholderia lata 383
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
immobilized metal ion affinity chromatography (Ni2+), cleavage of the tag, gel filtration
Burkholderia lata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-glutamine + chorismate
-
725425
Burkholderia lata
(S)-2-amino-2-deoxyisochorismate + L-glutamate
-
-
-
?
L-glutamine + chorismate
-
725425
Burkholderia lata 383
(S)-2-amino-2-deoxyisochorismate + L-glutamate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals
Burkholderia lata
Synonyms
Synonyms
Commentary
Organism
2-amino-2desoxyisochorismate synthase
-
Burkholderia lata
PhzE
-
Burkholderia lata
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.01
-
chorismate
pH 7.0, 25°C
Burkholderia lata
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged protein expressed in Escherichia coli BL21(DE3)
Burkholderia lata
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging-drop vapor diffusion method, PEG 3350
Burkholderia lata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mn2+
reduced activity
Burkholderia lata
Ni2+
reduced activity
Burkholderia lata
Zn2+
reduced activity
Burkholderia lata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0024
-
chorismate
pH 7.0, 25°C
Burkholderia lata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Burkholderia lata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals
Burkholderia lata
Purification (Commentary) (protein specific)
Commentary
Organism
immobilized metal ion affinity chromatography (Ni2+), cleavage of the tag, gel filtration
Burkholderia lata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-glutamine + chorismate
-
725425
Burkholderia lata
(S)-2-amino-2-deoxyisochorismate + L-glutamate
-
-
-
?
L-glutamine + chorismate
-
725425
Burkholderia lata 383
(S)-2-amino-2-deoxyisochorismate + L-glutamate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals
Burkholderia lata
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.01
-
chorismate
pH 7.0, 25°C
Burkholderia lata
Other publictions for EC 2.6.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737705
Culbertson
Conversion of aminodeoxychoris ...
Escherichia coli
Biochemistry
54
2372-2384
2015
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
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-
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2
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-
1
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3
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-
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-
-
-
724464
Culbertson
Expression and characterizatio ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
1834
240-246
2013
-
-
1
-
-
-
-
1
-
1
1
-
-
5
-
-
1
-
-
-
-
-
1
1
2
1
1
-
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
1
1
1
1
-
2
1
1
-
-
-
-
-
-
-
-
725425
Li
Ligand binding induces an ammo ...
Burkholderia lata, Burkholderia lata 383
J. Biol. Chem.
286
18213-18221
2011
-
-
1
1
-
-
3
1
-
1
1
-
-
4
-
-
1
-
-
-
-
-
2
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
3
-
1
-
1
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
682589
Van Lanen
Biosynthesis of the enediyne a ...
Streptomyces globisporus
Proc. Natl. Acad. Sci. USA
105
494-499
2008
-
-
1
-
-
-
-
-
-
1
1
1
-
1
-
-
1
-
-
-
-
-
2
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680341
McDonald
Phenazine biosynthesis in Pseu ...
Pseudomonas fluorescens
J. Am. Chem. Soc.
123
9459-9460
2001
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
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1
-
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2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
33303
Morollo
Characterization of composite ...
Salmonella enterica subsp. enterica serovar Typhimurium
Proc. Natl. Acad. Sci. USA
90
9983-9987
1993
-
-
-
-
-
-
-
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2
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1
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1
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