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show all sequences of 2.6.1.86

Expression and characterization of PhzE from P. aeruginosa PAO1: aminodeoxyisochorismate synthase involved in pyocyanin and phenazine-1-carboxylate production

Culbertson, J.E.; Toney, M.D.; Biochim. Biophys. Acta 1834, 240-246 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
chorismate
L-glutamine as ammonia source, in the presence of Mg2+, pH 7.0, 25°C
Pseudomonas aeruginosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
lower activity with Mn2+, Co2+, no detectable activity with Zn2+, Fe2+, Ca2+
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71251
-
x * 71251, His-tagged PhzE, MALDI-TOF
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
G3XCK6
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-glutamine + chorismate
-
724464
Pseudomonas aeruginosa
(S)-2-amino-2-deoxyisochorismate + glutamate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 71251, His-tagged PhzE, MALDI-TOF
Pseudomonas aeruginosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
30
-
Pseudomonas aeruginosa
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
45
-
Pseudomonas aeruginosa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.16
-
chorismate
ammonium sulfate as ammonia source, pH 7.0, 25°C
Pseudomonas aeruginosa
2.2
-
chorismate
L-glutamine as ammonia source, pH 7.0, 25°C
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
optimal activity at low pH values between pH 6.5 and pH 8.5
Pseudomonas aeruginosa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
chorismate
L-glutamine as ammonia source, in the presence of Mg2+, pH 7.0, 25°C
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
lower activity with Mn2+, Co2+, no detectable activity with Zn2+, Fe2+, Ca2+
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71251
-
x * 71251, His-tagged PhzE, MALDI-TOF
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-glutamine + chorismate
-
724464
Pseudomonas aeruginosa
(S)-2-amino-2-deoxyisochorismate + glutamate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 71251, His-tagged PhzE, MALDI-TOF
Pseudomonas aeruginosa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
30
-
Pseudomonas aeruginosa
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
45
-
Pseudomonas aeruginosa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.16
-
chorismate
ammonium sulfate as ammonia source, pH 7.0, 25°C
Pseudomonas aeruginosa
2.2
-
chorismate
L-glutamine as ammonia source, pH 7.0, 25°C
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
optimal activity at low pH values between pH 6.5 and pH 8.5
Pseudomonas aeruginosa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
-
Pseudomonas aeruginosa
Other publictions for EC 2.6.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737705
Culbertson
Conversion of aminodeoxychoris ...
Escherichia coli
Biochemistry
54
2372-2384
2015
-
1
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3
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724464
Culbertson
Expression and characterizatio ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
1834
240-246
2013
-
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1
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1
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1
1
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5
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1
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1
1
1
1
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2
1
1
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1
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1
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1
1
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1
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1
1
1
1
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2
1
1
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725425
Li
Ligand binding induces an ammo ...
Burkholderia lata, Burkholderia lata 383
J. Biol. Chem.
286
18213-18221
2011
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1
1
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3
1
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1
1
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4
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1
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2
1
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1
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1
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1
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3
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1
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1
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1
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2
1
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1
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682589
Van Lanen
Biosynthesis of the enediyne a ...
Streptomyces globisporus
Proc. Natl. Acad. Sci. USA
105
494-499
2008
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1
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1
1
1
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1
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1
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1
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680341
McDonald
Phenazine biosynthesis in Pseu ...
Pseudomonas fluorescens
J. Am. Chem. Soc.
123
9459-9460
2001
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33303
Morollo
Characterization of composite ...
Salmonella enterica subsp. enterica serovar Typhimurium
Proc. Natl. Acad. Sci. USA
90
9983-9987
1993
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