BRENDA - Enzyme Database show
show all sequences of 2.6.1.86

Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism

Van Lanen, S.G.; Lin, S.; Shen, B.; Proc. Natl. Acad. Sci. USA 105, 494-499 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the gene for SgcD is cloned into pET-30 Xa/LIC and expressed in Escherichia coli
Streptomyces globisporus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
SgcD activity is depended on the inclusion of Mg2+
Streptomyces globisporus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58500
-
x * 58500, SDS-PAGE
Streptomyces globisporus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
chorismate + L-glutamine
Streptomyces globisporus
the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic
(2S)-2-amino-4-deoxychorismate + L-glutamate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces globisporus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces globisporus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chorismate + L-glutamine
-
682589
Streptomyces globisporus
(2S)-2-amino-4-deoxychorismate + L-glutamate
-
-
-
?
chorismate + L-glutamine
the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic
682589
Streptomyces globisporus
(2S)-2-amino-4-deoxychorismate + L-glutamate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 58500, SDS-PAGE
Streptomyces globisporus
Cloned(Commentary) (protein specific)
Commentary
Organism
the gene for SgcD is cloned into pET-30 Xa/LIC and expressed in Escherichia coli
Streptomyces globisporus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
SgcD activity is depended on the inclusion of Mg2+
Streptomyces globisporus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58500
-
x * 58500, SDS-PAGE
Streptomyces globisporus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
chorismate + L-glutamine
Streptomyces globisporus
the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic
(2S)-2-amino-4-deoxychorismate + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces globisporus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chorismate + L-glutamine
-
682589
Streptomyces globisporus
(2S)-2-amino-4-deoxychorismate + L-glutamate
-
-
-
?
chorismate + L-glutamine
the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic
682589
Streptomyces globisporus
(2S)-2-amino-4-deoxychorismate + L-glutamate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 58500, SDS-PAGE
Streptomyces globisporus
Other publictions for EC 2.6.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737705
Culbertson
Conversion of aminodeoxychoris ...
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3
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724464
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1
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1
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1
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5
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1
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1
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1
1
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1
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1
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2
1
1
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725425
Li
Ligand binding induces an ammo ...
Burkholderia lata, Burkholderia lata 383
J. Biol. Chem.
286
18213-18221
2011
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1
1
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3
1
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1
1
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4
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1
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1
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1
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1
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3
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1
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1
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1
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2
1
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1
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-
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682589
Van Lanen
Biosynthesis of the enediyne a ...
Streptomyces globisporus
Proc. Natl. Acad. Sci. USA
105
494-499
2008
-
-
1
-
-
-
-
-
-
1
1
1
-
1
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1
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2
1
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1
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1
1
1
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1
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2
1
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680341
McDonald
Phenazine biosynthesis in Pseu ...
Pseudomonas fluorescens
J. Am. Chem. Soc.
123
9459-9460
2001
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1
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33303
Morollo
Characterization of composite ...
Salmonella enterica subsp. enterica serovar Typhimurium
Proc. Natl. Acad. Sci. USA
90
9983-9987
1993
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