Cloned (Comment) | Organism |
---|---|
pabB, optimized for overexpression in Escherichia coli, His-tagged protein expressed in Escherichia coli BL21(DE3) | Stenotrophomonas maltophilia |
Crystallization (Comment) | Organism |
---|---|
pH 7.8, polyethylene glycol monomethylether 550 | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
chorismate | PabB, pH 8.0, 25°C | Stenotrophomonas maltophilia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49500 | - |
PabB | Stenotrophomonas maltophilia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Co2+), fusion tag removed | Stenotrophomonas maltophilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-2-deoxychorismate + NH3 | PabB, second half of the reaction, starting with the intermediate | Stenotrophomonas maltophilia | 4-amino-4-deoxychorismate + NH3 | - |
? | |
chorismate + NH3 | PabB, intermediate 2-amino-2-deoxychorismate involved | Stenotrophomonas maltophilia | 4-amino-4-deoxychorismate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | PabB in solution | Stenotrophomonas maltophilia |
Synonyms | Comment | Organism |
---|---|---|
aminodeoxychorismate synthase | heterodimeric complex, subunit PabB catalyces the amination of chorismate, subunit PabA is an amidotransferase that supplies ammonia from glutamine hydrolysis, subunit PabA is not identfied in Stenotrophomonas maltophilia | Stenotrophomonas maltophilia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
chorismate | PabB, addition of Escherichia coli PabA enhances the reaction rate 20fold in the absence of glutamine, pH 8.0, 25°C | Stenotrophomonas maltophilia | |
0.02 | - |
2-amino-2-deoxychorismate | PabB, second half of the reaction, pH 8.0, 25°C | Stenotrophomonas maltophilia |