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Literature summary for 2.6.1.85 extracted from

  • Bera, A.K.; Atanasova, V.; Dhanda, A.; Ladner, J.E.; Parsons, J.F.
    Structure of aminodeoxychorismate synthase from Stenotrophomonas maltophilia (2012), Biochemistry, 51, 10208-10217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
pabB, optimized for overexpression in Escherichia coli, His-tagged protein expressed in Escherichia coli BL21(DE3) Stenotrophomonas maltophilia

Crystallization (Commentary)

Crystallization (Comment) Organism
pH 7.8, polyethylene glycol monomethylether 550 Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
chorismate PabB, pH 8.0, 25°C Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49500
-
PabB Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Co2+), fusion tag removed Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2-deoxychorismate + NH3 PabB, second half of the reaction, starting with the intermediate Stenotrophomonas maltophilia 4-amino-4-deoxychorismate + NH3
-
?
chorismate + NH3 PabB, intermediate 2-amino-2-deoxychorismate involved Stenotrophomonas maltophilia 4-amino-4-deoxychorismate + ?
-
?

Subunits

Subunits Comment Organism
monomer PabB in solution Stenotrophomonas maltophilia

Synonyms

Synonyms Comment Organism
aminodeoxychorismate synthase heterodimeric complex, subunit PabB catalyces the amination of chorismate, subunit PabA is an amidotransferase that supplies ammonia from glutamine hydrolysis, subunit PabA is not identfied in Stenotrophomonas maltophilia Stenotrophomonas maltophilia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
chorismate PabB, addition of Escherichia coli PabA enhances the reaction rate 20fold in the absence of glutamine, pH 8.0, 25°C Stenotrophomonas maltophilia
0.02
-
2-amino-2-deoxychorismate PabB, second half of the reaction, pH 8.0, 25°C Stenotrophomonas maltophilia