BRENDA - Enzyme Database show
show all sequences of 2.6.1.85

Structure of aminodeoxychorismate synthase from Stenotrophomonas maltophilia

Bera, A.K.; Atanasova, V.; Dhanda, A.; Ladner, J.E.; Parsons, J.F.; Biochemistry 51, 10208-10217 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
pabB, optimized for overexpression in Escherichia coli, His-tagged protein expressed in Escherichia coli BL21(DE3)
Stenotrophomonas maltophilia
Crystallization (Commentary)
Crystallization
Organism
pH 7.8, polyethylene glycol monomethylether 550
Stenotrophomonas maltophilia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
chorismate
PabB, pH 8.0, 25°C
Stenotrophomonas maltophilia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49500
-
PabB
Stenotrophomonas maltophilia
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Stenotrophomonas maltophilia
-
-
-
Purification (Commentary)
Commentary
Organism
immobilized metal ion affinity chromatography (Co2+), fusion tag removed
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-2-deoxychorismate + NH3
PabB, second half of the reaction, starting with the intermediate
724331
Stenotrophomonas maltophilia
4-amino-4-deoxychorismate + NH3
-
-
-
?
chorismate + NH3
PabB, intermediate 2-amino-2-deoxychorismate involved
724331
Stenotrophomonas maltophilia
4-amino-4-deoxychorismate + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
PabB in solution
Stenotrophomonas maltophilia
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.007
-
chorismate
PabB, addition of Escherichia coli PabA enhances the reaction rate 20fold in the absence of glutamine, pH 8.0, 25°C
Stenotrophomonas maltophilia
0.02
-
2-amino-2-deoxychorismate
PabB, second half of the reaction, pH 8.0, 25°C
Stenotrophomonas maltophilia
Cloned(Commentary) (protein specific)
Commentary
Organism
pabB, optimized for overexpression in Escherichia coli, His-tagged protein expressed in Escherichia coli BL21(DE3)
Stenotrophomonas maltophilia
Crystallization (Commentary) (protein specific)
Crystallization
Organism
pH 7.8, polyethylene glycol monomethylether 550
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
chorismate
PabB, pH 8.0, 25°C
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49500
-
PabB
Stenotrophomonas maltophilia
Purification (Commentary) (protein specific)
Commentary
Organism
immobilized metal ion affinity chromatography (Co2+), fusion tag removed
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-2-deoxychorismate + NH3
PabB, second half of the reaction, starting with the intermediate
724331
Stenotrophomonas maltophilia
4-amino-4-deoxychorismate + NH3
-
-
-
?
chorismate + NH3
PabB, intermediate 2-amino-2-deoxychorismate involved
724331
Stenotrophomonas maltophilia
4-amino-4-deoxychorismate + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
PabB in solution
Stenotrophomonas maltophilia
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.007
-
chorismate
PabB, addition of Escherichia coli PabA enhances the reaction rate 20fold in the absence of glutamine, pH 8.0, 25°C
Stenotrophomonas maltophilia
0.02
-
2-amino-2-deoxychorismate
PabB, second half of the reaction, pH 8.0, 25°C
Stenotrophomonas maltophilia
Other publictions for EC 2.6.1.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737705
Culbertson
Conversion of aminodeoxychoris ...
Escherichia coli
Biochemistry
54
2372-2384
2015
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1
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738864
Deng
Cloning, expression, and chara ...
Agaricus bisporus
J. Microbiol. Biotechnol.
25
66-73
2015
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1
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2
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1
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3
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1
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1
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2
2
724464
Culbertson
Expression and characterizatio ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
1834
240-246
2013
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4
3
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3
1
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5
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3
1
1
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2
1
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4
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1
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3
1
1
1
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2
1
-
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-
724331
Bera
Structure of aminodeoxychorism ...
Stenotrophomonas maltophilia
Biochemistry
51
10208-10217
2012
-
-
1
1
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1
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1
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3
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1
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2
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724111
Camara
The synthesis of pABA: Couplin ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
505
83-90
2011
1
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1
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1
7
1
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3
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1
4
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7
1
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1
4
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6
6
702371
Ziebart
Nucleophile specificity in ant ...
Escherichia coli
Biochemistry
49
2851-2859
2010
-
-
1
-
2
-
-
-
-
-
-
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1
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2
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684435
Keller
Action of atrop-abyssomicin C ...
Bacillus subtilis
Angew. Chem. Int. Ed. Engl.
46
8284-8286
2007
-
-
-
-
-
-
1
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661933
He
Conservation of mechanism in t ...
Escherichia coli
J. Am. Chem. Soc.
126
2378-2385
2004
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1
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2
-
1
5
-
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3
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1
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2
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6
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2
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1
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2
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1
2
5
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2
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6
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661938
Bulloch
Identification of 4-amino-4-de ...
Escherichia coli
J. Am. Chem. Soc.
126
9912-9913
2004
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1
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644249
Parsons
Structure of Escherichia coli ...
Escherichia coli
Biochemistry
41
2198-2208
2002
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1
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1
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1
2
1
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644250
Chang
p-Aminobenzoic acid and chlora ...
Streptomyces venezuelae, Streptomyces venezuelae ISP5230
Microbiology
147
2113-2126
2001
-
-
1
-
-
-
-
-
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2
-
8
-
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2
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644248
Rayl
Escherichia coli aminodeoxycho ...
Escherichia coli
Biochim. Biophys. Acta
1295
81-88
1996
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644230
Viswanathan
Kinetic characterization of 4- ...
Escherichia coli
J. Bacteriol.
177
5918-5923
1995
2
-
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1
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2
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644227
Ye
p-Aminobenzoate synthesis in E ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
87
9391-9395
1990
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