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Literature summary for 2.6.1.85 extracted from

  • Camara, D.; Richefeu-Contesto, C.; Gambonnet, B.; Dumas, R.; Rebeille, F.
    The synthesis of pABA: Coupling between the glutamine amidotransferase and aminodeoxychorismate synthase domains of the bifunctional aminodeoxychorismate synthase from Arabidopsis thaliana (2011), Arch. Biochem. Biophys., 505, 83-90.
    View publication on PubMed
Search for more literature for 2.6.1.85

Activating Compound

Activating Compound Comment Organism Structure
chorismate about 50% activation at 0.002 mM Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein (first 43 amino acid residues deleted) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
NH3 inhibitor of the glutamine amidotransferase reaction (Ki = 5 mM, lowered to 0.27 mM if considered that NH3 and not NH4+ is the true inhibitor (pKa value of 9.24)) Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 chorismate apparent K0.5 of chorismate for the activation of glutamine amidotransferase (calculated from chorismate-dependent activation), pH 8.0, 37°C Arabidopsis thaliana
0.0022
-
 chorismate p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana
0.009
-
 chorismate NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana
0.045
-
 L-glutamine glutamate production in the presence of chorismate, pH 8.0, 37°C Arabidopsis thaliana
0.05
-
 L-glutamine glutamate production in the absence of chorismate, pH 8.0, 37°C Arabidopsis thaliana
0.064
-
 L-glutamine p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana
195
-
 NH3 NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Arabidopsis thaliana 9536
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+), gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-glutamine
-
 Arabidopsis thaliana L-glutamate + 4-amino-4-deoxychorismate
-
 ?
chorismate + NH3
-
 Arabidopsis thaliana 4-amino-4-deoxychorismate + ?
-
 ?

Synonyms

Synonyms Comment Organism
aminodeoxychorismate synthase
-
 Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 at least stable for 30 min Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 NH3 NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana
0.22
-
 L-glutamine p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana
0.23
-
 L-glutamine glutamate production in the absence of chorismate, pH 8.0, 37°C Arabidopsis thaliana
0.79
-
 L-glutamine glutamate production in the presence of chorismate, pH 8.0, 37°C Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5
-
 NH3 inhibitor of the glutamine amidotransferase reaction (Ki lowered to 0.27 mM if considered that NH3 and not NH4+ is the true inhibitor (pKa value of 9.24)), pH 8.0, 37°C Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00061
-
 NH3 NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana
3.4
-
 L-glutamine p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana
4.6
-
 L-glutamine glutamate production in the absence of chorismate, pH 8.0, 37°C Arabidopsis thaliana
13.3
-
 chorismate NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana
17.5
-
 L-glutamine glutamate production in the presence of chorismate, pH 8.0, 37°C Arabidopsis thaliana
100
-
 chorismate p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C Arabidopsis thaliana