BRENDA - Enzyme Database show
show all sequences of 2.6.1.85

Nucleophile specificity in anthranilate synthase, aminodeoxychorismate synthase, isochorismate synthase, and salicylate synthase

Ziebart, K.T.; Toney, M.D.; Biochemistry 49, 2851-2859 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
K213A
subunit PabB, numbering used is based on the EntC protein from Escherichia coli, reduced activity
Escherichia coli
K213A/Q147K
subunit PabB, numbering used is based on the EntC protein from Escherichia coli, strongly reduced activity
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chorismate + L-glutamine
-
702371
Escherichia coli
L-glutamate + 4-amino-4-deoxychorismate
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
PabA-PabB
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.6
-
L-glutamine
mutant K213A/Q147K amidotransferase activity, pH 8.0, 25°C
Escherichia coli
0.67
-
L-glutamine
wild type amidotransferase activity, pH 8.0, 25°C
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
K213A
subunit PabB, numbering used is based on the EntC protein from Escherichia coli, reduced activity
Escherichia coli
K213A/Q147K
subunit PabB, numbering used is based on the EntC protein from Escherichia coli, strongly reduced activity
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chorismate + L-glutamine
-
702371
Escherichia coli
L-glutamate + 4-amino-4-deoxychorismate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
PabA-PabB
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.6
-
L-glutamine
mutant K213A/Q147K amidotransferase activity, pH 8.0, 25°C
Escherichia coli
0.67
-
L-glutamine
wild type amidotransferase activity, pH 8.0, 25°C
Escherichia coli
Other publictions for EC 2.6.1.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737705
Culbertson
Conversion of aminodeoxychoris ...
Escherichia coli
Biochemistry
54
2372-2384
2015
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1
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738864
Deng
Cloning, expression, and chara ...
Agaricus bisporus
J. Microbiol. Biotechnol.
25
66-73
2015
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1
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1
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1
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2
2
724464
Culbertson
Expression and characterizatio ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
1834
240-246
2013
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4
3
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3
1
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5
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3
1
1
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2
1
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4
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3
1
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3
1
1
1
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2
1
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724331
Bera
Structure of aminodeoxychorism ...
Stenotrophomonas maltophilia
Biochemistry
51
10208-10217
2012
-
-
1
1
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1
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1
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3
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1
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1
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2
1
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2
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724111
Camara
The synthesis of pABA: Couplin ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
505
83-90
2011
1
-
1
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1
7
1
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3
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1
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1
4
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1
1
7
1
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1
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2
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1
4
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6
6
702371
Ziebart
Nucleophile specificity in ant ...
Escherichia coli
Biochemistry
49
2851-2859
2010
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1
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684435
Keller
Action of atrop-abyssomicin C ...
Bacillus subtilis
Angew. Chem. Int. Ed. Engl.
46
8284-8286
2007
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661933
He
Conservation of mechanism in t ...
Escherichia coli
J. Am. Chem. Soc.
126
2378-2385
2004
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1
5
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5
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661938
Bulloch
Identification of 4-amino-4-de ...
Escherichia coli
J. Am. Chem. Soc.
126
9912-9913
2004
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644249
Parsons
Structure of Escherichia coli ...
Escherichia coli
Biochemistry
41
2198-2208
2002
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1
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1
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644250
Chang
p-Aminobenzoic acid and chlora ...
Streptomyces venezuelae, Streptomyces venezuelae ISP5230
Microbiology
147
2113-2126
2001
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1
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2
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8
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644248
Rayl
Escherichia coli aminodeoxycho ...
Escherichia coli
Biochim. Biophys. Acta
1295
81-88
1996
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644230
Viswanathan
Kinetic characterization of 4- ...
Escherichia coli
J. Bacteriol.
177
5918-5923
1995
2
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1
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644227
Ye
p-Aminobenzoate synthesis in E ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
87
9391-9395
1990
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