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Literature summary for 2.6.1.83 extracted from
- L,L-diaminopimelate aminotransferase from Chlamydomonas reinhardtii: a target for algaecide development (2011), PLoS ONE, 6, e20439.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| S- and His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL, also used in complementation experiments | Chlamydomonas reinhardtii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| PEG 3350, sitting drop method | Chlamydomonas reinhardtii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
tetrahydrodipicolinate | pH 7.6, 30°C | Chlamydomonas reinhardtii |  |
| 0.3 | - |
LL-diaminopimelate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
| 0.9 | - |
L-glutamate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
| 2.2 | - |
2-oxoglutarate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE, analytical ultracentrifugation | Chlamydomonas reinhardtii |
| 100200 | - |
analytical ultracentrifugation | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | A8IW39 | - |
- |
| Chlamydomonas reinhardtii CC-1690 | A8IW39 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography, gel filtration | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.68 | - |
substrates glutamate and tetrahydrodipicolinate, pH 7.6, 30°C | Chlamydomonas reinhardtii |
| 11.6 | - |
substrates LL-diaminopimelate and 2-oxoglutarate, pH 7.6, 30°C | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + tetrahydrodipicolinate + H2O | no activity observed with pyruvate, prephenate, oxaloacetate and oxovalerate | Chlamydomonas reinhardtii | LL-diaminopimelate + 2-oxoglutarate | - |
r | |
| L-glutamate + tetrahydrodipicolinate + H2O | no activity observed with pyruvate, prephenate, oxaloacetate and oxovalerate | Chlamydomonas reinhardtii CC-1690 | LL-diaminopimelate + 2-oxoglutarate | - |
r | |
| LL-diaminopimelate + 2-oxoglutarate | no activity observed with meso-diaminopimelate, L-lysine and L-ornithine | Chlamydomonas reinhardtii | L-glutamate + tetrahydrodipicolinate + H2O | - |
r | |
| LL-diaminopimelate + 2-oxoglutarate | no activity observed with meso-diaminopimelate, L-lysine and L-ornithine | Chlamydomonas reinhardtii CC-1690 | L-glutamate + tetrahydrodipicolinate + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, SDS-PAGE, analytical ultracentrifugation | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L,L-diaminopimelate aminotransferase | - |
Chlamydomonas reinhardtii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
tetrahydrodipicolinate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
| 19 | - |
LL-diaminopimelate | pH 7.6, 30°C | Chlamydomonas reinhardtii | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Chlamydomonas reinhardtii | |
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