Cloned (Comment) | Organism |
---|---|
His-tagged protein (amino acid residue 21-456) expressed in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
PEG 3350 | Arabidopsis thaliana |
to 2.3 A resolution. The large donain (Pro85Ser338) consists of 254 residues and has an alpha-beta-alpha sandwich fold. The large domain includes both the pyridoxal phosphate-binding site and the dimerization site. The small domain is composed of the remaining residues, Gly34Ile84 and Ser339Thr441. The small domain has an alpha/beta architecture, with a beta-sheet surrounded by two outer layers alpha-helices | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9ZQI7 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), gel filtration | Arabidopsis thaliana |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
Ald1 | - |
Arabidopsis thaliana |
diaminopimelate aminotransferase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Arabidopsis thaliana |