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Literature summary for 2.6.1.82 extracted from
- Molecular cloning and characterization of Escherichia coli K12 ygjG gene (2003), BMC Microbiol., 3, 2.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli TG1 and BL21 (DE3) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.2 | - |
putrescine | - |
Escherichia coli |  |
| 19 | - |
2-oxoglutaric acid | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
SDS-PAGE, 49600 Da for ORF2 coding protein and 2100 Da for His6-tag leader peptide | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P42588 | K12 | - |
| Escherichia coli | P42588 | MG1655 (K-12 lambda-F-) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 95% purified by immobilized-metal affinity chromatography | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4-aminobutanal = 1-pyrroline + H2O | (1b) | Escherichia coli | |
| putrescine + 2-oxoglutarate = 4-aminobutanal + L-glutamate | (1a) | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.047 | - |
highest activity in cell extracts grown under nitrogen limitation | Escherichia coli |
| 2.16 | - |
essential level of glutamate formation activity in crude cells of BL21(DE3) harboring pET-Ht-ORF2 | Escherichia coli |
| 11.68 | - |
purified enzyme with yield of 81% | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, phosphate buffer, pH 7.4, 15% glycerol, until required | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + 2-oxoglutarate | 2% activity | Escherichia coli | ? | - |
? | |
| alpha-ketobutyric acid + putrescine | 38% activity | Escherichia coli | L-aspartate + 4-aminobutanal | - |
? | |
| cadaverine + 2-oxoglutarate | 97% activity | Escherichia coli | L-glutamate + 5-aminopentanal | - |
? | |
| L-ornithine + 2-oxoglutarate | 2% activity | Escherichia coli | ? | - |
? | |
| putrescine + 2-oxoglutarate | putrescine best amino group donor for the enzyme, 100% activity | Escherichia coli | L-glutamate + 4-aminobutanal | - |
? | |
| pyruvate + putrescine | 12% activity | Escherichia coli | L-alanine + 4-aminobutanal | - |
? | |
| spermidine + 2-oxoglutarate | 32% activity | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PATase | - |
Escherichia coli |
| YgjG | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
in Tris-HCl buffer | Escherichia coli |
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