BRENDA - Enzyme Database show
show all sequences of 2.6.1.79

Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms

Graindorge, M.; Giustini, C.; Kraut, A.; Moyet, L.; Curien, G.; Matringe, M.; J. Biol. Chem. 289, 3198-3208 (2014)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
prephenate
cosubstrate L-glutamate, pH 8.0, 30C
Streptomyces avermitilis
0.021
-
prephenate
cosubstrate N-succinyl-L-2,6-diaminoheptanedioate, pH 8.0, 30C
Streptomyces avermitilis
0.1
-
prephenate
pH 8.0, 30C
Sinorhizobium meliloti
0.13
-
prephenate
pH 8.0, 30C
Synechocystis sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sinorhizobium meliloti
Q02635
cf. EC 2.6.1.1
-
Streptomyces avermitilis
Q82IK5
cf. EC 2.6.1.17
-
Streptomyces avermitilis DSM 46492
Q82IK5
cf. EC 2.6.1.17
-
Synechocystis sp.
P54691
cf. EC 2.6.1.42
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
prephenate + L-glutamate
-
738632
Streptomyces avermitilis
L-arogenate + 2-oxoglutarate
-
-
-
?
prephenate + L-glutamate
-
738632
Sinorhizobium meliloti
L-arogenate + 2-oxoglutarate
-
-
-
?
prephenate + L-glutamate
-
738632
Synechocystis sp.
L-arogenate + 2-oxoglutarate
-
-
-
?
prephenate + L-glutamate
-
738632
Streptomyces avermitilis DSM 46492
L-arogenate + 2-oxoglutarate
-
-
-
?
prephenate + N-succinyl-L-2,6-diaminoheptanedioate
-
738632
Streptomyces avermitilis
L-arogenate + N-succinyl-2-amino-6-oxoheptanedioate
-
-
-
?
prephenate + N-succinyl-L-2,6-diaminoheptanedioate
-
738632
Streptomyces avermitilis DSM 46492
L-arogenate + N-succinyl-2-amino-6-oxoheptanedioate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
24
-
prephenate
cosubstrate N-succinyl-L-2,6-diaminoheptanedioate, pH 8.0, 30C
Streptomyces avermitilis
43
-
prephenate
pH 8.0, 30C
Sinorhizobium meliloti
54
-
prephenate
cosubstrate L-glutamate, pH 8.0, 30C
Streptomyces avermitilis
60
-
prephenate
pH 8.0, 30C
Synechocystis sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
prephenate
cosubstrate L-glutamate, pH 8.0, 30C
Streptomyces avermitilis
0.021
-
prephenate
cosubstrate N-succinyl-L-2,6-diaminoheptanedioate, pH 8.0, 30C
Streptomyces avermitilis
0.1
-
prephenate
pH 8.0, 30C
Sinorhizobium meliloti
0.13
-
prephenate
pH 8.0, 30C
Synechocystis sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
prephenate + L-glutamate
-
738632
Streptomyces avermitilis
L-arogenate + 2-oxoglutarate
-
-
-
?
prephenate + L-glutamate
-
738632
Sinorhizobium meliloti
L-arogenate + 2-oxoglutarate
-
-
-
?
prephenate + L-glutamate
-
738632
Synechocystis sp.
L-arogenate + 2-oxoglutarate
-
-
-
?
prephenate + L-glutamate
-
738632
Streptomyces avermitilis DSM 46492
L-arogenate + 2-oxoglutarate
-
-
-
?
prephenate + N-succinyl-L-2,6-diaminoheptanedioate
-
738632
Streptomyces avermitilis
L-arogenate + N-succinyl-2-amino-6-oxoheptanedioate
-
-
-
?
prephenate + N-succinyl-L-2,6-diaminoheptanedioate
-
738632
Streptomyces avermitilis DSM 46492
L-arogenate + N-succinyl-2-amino-6-oxoheptanedioate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
24
-
prephenate
cosubstrate N-succinyl-L-2,6-diaminoheptanedioate, pH 8.0, 30C
Streptomyces avermitilis
43
-
prephenate
pH 8.0, 30C
Sinorhizobium meliloti
54
-
prephenate
cosubstrate L-glutamate, pH 8.0, 30C
Streptomyces avermitilis
60
-
prephenate
pH 8.0, 30C
Synechocystis sp.
Other publictions for EC 2.6.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738632
Graindorge
Three different classes of ami ...
Sinorhizobium meliloti, Streptomyces avermitilis, Streptomyces avermitilis DSM 46492, Synechocystis sp.
J. Biol. Chem.
289
3198-3208
2014
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
739351
de la Torre
Deciphering the role of aspart ...
Nicotiana benthamiana
Plant Physiol.
164
92-104
2014
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722267
Graindorge
Identification of a plant gene ...
Arabidopsis thaliana
FEBS Lett.
584
4357-4360
2010
-
-
1
-
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
659858
Bonner
Prephenate aminotransferase ...
Brevibacterium flavum, Corynebacterium glutamicum, Hemerocallis hybrid cultivar, Lemna sp., Magnolia sp., Nicotiana sylvestris, Sorghum sp., Spinacia oleracea
Methods Enzymol.
142
479-487
1987
-
-
-
-
-
1
1
-
-
-
1
8
-
8
-
-
3
-
-
15
2
1
37
-
1
1
8
-
1
-
-
8
-
-
-
-
-
-
8
-
-
1
-
1
-
-
-
-
1
8
-
-
-
3
-
15
2
1
37
-
1
1
8
-
1
-
-
-
-
-
-
-
-
-
660563
Siehl
Tyrosine biosynthesis in Sorgh ...
Sorghum bicolor x Sorghum sudanesis
Z. Naturforsch. C
41
79-86
1986
-
-
-
-
-
1
-
3
-
-
-
1
-
4
-
-
1
-
-
1
1
-
6
-
1
-
1
-
1
1
-
1
-
1
-
-
-
-
1
-
-
1
-
-
-
3
-
-
-
1
-
-
-
1
-
1
1
-
6
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
657635
Bonner
Novel features of prephenate a ...
Nicotiana sylvestris
Arch. Biochem. Biophys.
238
237-246
1985
-
-
-
-
-
1
1
-
-
-
-
1
-
1
-
-
1
-
-
1
9
1
9
-
1
1
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
-
1
-
1
9
1
9
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-