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Literature summary for 2.6.1.79 extracted from
- Prephenate aminotransferase (1987), Methods Enzymol., 142, 479-487.
General Stability
| General Stability | Organism |
|---|---|
| pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay | Nicotiana sylvestris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| prephenate | substrate inhibition above 1 mM, 80% of maximal activity at 2 mM, 38% of maximal activity at 20 mM | Nicotiana sylvestris |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
gel filtration | Nicotiana sylvestris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate + L-glutamate | Spinacia oleracea | - |
L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | [Brevibacterium] flavum | - |
L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | Corynebacterium glutamicum | - |
L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | Nicotiana sylvestris | - |
L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | Sorghum sp. | - |
L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | Magnolia sp. | - |
L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | Lemna sp. | - |
L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | Hemerocallis hybrid cultivar | - |
L-arogenate + 2-oxoglutarate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
| Hemerocallis hybrid cultivar | - |
day lily | - |
| Lemna sp. | - |
duckweed | - |
| Magnolia sp. | - |
- |
- |
| Nicotiana sylvestris | - |
- |
- |
| Sorghum sp. | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
| [Brevibacterium] flavum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 252fold from cell culture by ammonium sulfate fractionation, heat treatment, affinity chromatography, and gel filtration | Nicotiana sylvestris |
| to homogeneity | [Brevibacterium] flavum |
| to homogeneity | Corynebacterium glutamicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Spinacia oleracea | - |
| cell culture | - |
[Brevibacterium] flavum | - |
| cell culture | - |
Corynebacterium glutamicum | - |
| cell culture | - |
Nicotiana sylvestris | - |
| cell culture | - |
Sorghum sp. | - |
| cell culture | - |
Lemna sp. | - |
| cell culture | - |
Hemerocallis hybrid cultivar | - |
| leaf | - |
Spinacia oleracea | - |
| leaf | - |
[Brevibacterium] flavum | - |
| leaf | - |
Corynebacterium glutamicum | - |
| leaf | - |
Nicotiana sylvestris | - |
| leaf | - |
Sorghum sp. | - |
| leaf | - |
Magnolia sp. | - |
| leaf | - |
Lemna sp. | - |
| leaf | - |
Hemerocallis hybrid cultivar | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
different assay methods using L-glutamate or L-aspartate, development of coupled assay methods, overview | Nicotiana sylvestris |
| 8.3 | - |
purified enzyme | Nicotiana sylvestris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, purified enzyme is stable for at least 1 month, partially purified enzyme is stable for at least several months | Nicotiana sylvestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylpyruvate + L-glutamate | - |
Spinacia oleracea | L-tyrosine + 2-oxoglutarate | - |
? | |
| 4-hydroxyphenylpyruvate + L-glutamate | - |
[Brevibacterium] flavum | L-tyrosine + 2-oxoglutarate | - |
? | |
| 4-hydroxyphenylpyruvate + L-glutamate | - |
Corynebacterium glutamicum | L-tyrosine + 2-oxoglutarate | - |
? | |
| 4-hydroxyphenylpyruvate + L-glutamate | - |
Sorghum sp. | L-tyrosine + 2-oxoglutarate | - |
? | |
| 4-hydroxyphenylpyruvate + L-glutamate | - |
Magnolia sp. | L-tyrosine + 2-oxoglutarate | - |
? | |
| 4-hydroxyphenylpyruvate + L-glutamate | - |
Lemna sp. | L-tyrosine + 2-oxoglutarate | - |
? | |
| 4-hydroxyphenylpyruvate + L-glutamate | - |
Hemerocallis hybrid cultivar | L-tyrosine + 2-oxoglutarate | - |
? | |
| 4-hydroxyphenylpyruvate + L-glutamate | about 10% of the activity with prephenate | Nicotiana sylvestris | L-tyrosine + 2-oxoglutarate | - |
r | |
| additional information | no activity with oxaloacetate, 2-ketoglutarate, or pyruvate with L-glutamate | Nicotiana sylvestris | ? | - |
? | |
| additional information | the substrate specificity of the enzyme in bacteria is less high than in plants | [Brevibacterium] flavum | ? | - |
? | |
| additional information | the substrate specificity of the enzyme in bacteria is less high than in plants | Corynebacterium glutamicum | ? | - |
? | |
| phenylpyruvate + L-glutamate | - |
Spinacia oleracea | L-phenylalanine + 2-oxoglutarate | - |
? | |
| phenylpyruvate + L-glutamate | - |
[Brevibacterium] flavum | L-phenylalanine + 2-oxoglutarate | - |
? | |
| phenylpyruvate + L-glutamate | - |
Corynebacterium glutamicum | L-phenylalanine + 2-oxoglutarate | - |
? | |
| phenylpyruvate + L-glutamate | - |
Sorghum sp. | L-phenylalanine + 2-oxoglutarate | - |
? | |
| phenylpyruvate + L-glutamate | - |
Magnolia sp. | L-phenylalanine + 2-oxoglutarate | - |
? | |
| phenylpyruvate + L-glutamate | - |
Lemna sp. | L-phenylalanine + 2-oxoglutarate | - |
? | |
| phenylpyruvate + L-glutamate | - |
Hemerocallis hybrid cultivar | L-phenylalanine + 2-oxoglutarate | - |
? | |
| phenylpyruvate + L-glutamate | about 5% of the activity with prephenate | Nicotiana sylvestris | L-phenylalanine + 2-oxoglutarate | - |
r | |
| prephenate + L-aspartate | about 50% of the activity with L-glutamate | Nicotiana sylvestris | L-arogenate + oxaloacetate | - |
r | |
| prephenate + L-glutamate | - |
Spinacia oleracea | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | - |
[Brevibacterium] flavum | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | - |
Corynebacterium glutamicum | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | - |
Nicotiana sylvestris | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | - |
Sorghum sp. | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | - |
Magnolia sp. | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | - |
Lemna sp. | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | - |
Hemerocallis hybrid cultivar | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | prephenate is the preferred substrate | Spinacia oleracea | L-arogenate + 2-oxoglutarate | L-arogenate is cyclohexadienylic acid | r | |
| prephenate + L-glutamate | prephenate is the preferred substrate | [Brevibacterium] flavum | L-arogenate + 2-oxoglutarate | L-arogenate is cyclohexadienylic acid | r | |
| prephenate + L-glutamate | prephenate is the preferred substrate | Corynebacterium glutamicum | L-arogenate + 2-oxoglutarate | L-arogenate is cyclohexadienylic acid | r | |
| prephenate + L-glutamate | prephenate is the preferred substrate | Sorghum sp. | L-arogenate + 2-oxoglutarate | L-arogenate is cyclohexadienylic acid | r | |
| prephenate + L-glutamate | prephenate is the preferred substrate | Magnolia sp. | L-arogenate + 2-oxoglutarate | L-arogenate is cyclohexadienylic acid | r | |
| prephenate + L-glutamate | prephenate is the preferred substrate | Lemna sp. | L-arogenate + 2-oxoglutarate | L-arogenate is cyclohexadienylic acid | r | |
| prephenate + L-glutamate | prephenate is the preferred substrate | Hemerocallis hybrid cultivar | L-arogenate + 2-oxoglutarate | L-arogenate is cyclohexadienylic acid | r | |
| prephenate + L-glutamate | prephenate is the preferred substrate, optimal at low concentrations of about 1 mM | Nicotiana sylvestris | L-arogenate + 2-oxoglutarate | L-arogenate is cyclohexadienylic acid | r | |
| pyruvate + L-glutamate | about 20% of the activity with prephenate | Nicotiana sylvestris | L-alanine + 2-oxoglutarate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| prephenate aminotransferase | - |
Spinacia oleracea |
| prephenate aminotransferase | - |
[Brevibacterium] flavum |
| prephenate aminotransferase | - |
Corynebacterium glutamicum |
| prephenate aminotransferase | - |
Nicotiana sylvestris |
| prephenate aminotransferase | - |
Sorghum sp. |
| prephenate aminotransferase | - |
Magnolia sp. |
| prephenate aminotransferase | - |
Lemna sp. |
| prephenate aminotransferase | - |
Hemerocallis hybrid cultivar |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Nicotiana sylvestris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | - |
Nicotiana sylvestris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable up to | Spinacia oleracea |
| 70 | - |
stable up to | [Brevibacterium] flavum |
| 70 | - |
stable up to | Corynebacterium glutamicum |
| 70 | - |
stable up to | Sorghum sp. |
| 70 | - |
stable up to | Magnolia sp. |
| 70 | - |
stable up to | Lemna sp. |
| 70 | - |
stable up to | Hemerocallis hybrid cultivar |
| 70 | - |
stable up to, 30 min | Nicotiana sylvestris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.6 | - |
Nicotiana sylvestris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Spinacia oleracea | |
| pyridoxal 5'-phosphate | - |
[Brevibacterium] flavum | |
| pyridoxal 5'-phosphate | - |
Corynebacterium glutamicum | |
| pyridoxal 5'-phosphate | - |
Sorghum sp. | |
| pyridoxal 5'-phosphate | - |
Magnolia sp. | |
| pyridoxal 5'-phosphate | - |
Lemna sp. | |
| pyridoxal 5'-phosphate | - |
Hemerocallis hybrid cultivar | |
| pyridoxal 5'-phosphate | tightly bound to the enzyme | Nicotiana sylvestris | |
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