BRENDA - Enzyme Database show
show all sequences of 2.6.1.79

Novel features of prephenate aminotransferase from cell cultures of Nicotiana silvestris

Bonner, C.A.; Jensen, R.A.; Arch. Biochem. Biophys. 238, 237-246 (1985)

Data extracted from this reference:

General Stability
General Stability
Organism
pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay
Nicotiana sylvestris
Inhibitors
Inhibitors
Commentary
Organism
Structure
prephenate
substrate inhibition above 1 mM, antagonism by L-glutamate
Nicotiana sylvestris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prephenate + L-glutamate
Nicotiana sylvestris
important step in biosynthesis of L-tyrosine and L-phenylalanine
L-arogenate + 2-oxoglutarate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana sylvestris
-
-
-
Purification (Commentary)
Commentary
Organism
partially by ion exchange chromatography
Nicotiana sylvestris
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
stable cell line ANS-1
Nicotiana sylvestris
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
substrate specificity
Nicotiana sylvestris
0.042
-
crude enzyme extract, substrates prephenate and L-glutamate
Nicotiana sylvestris
0.073
-
partially purified enzyme, substrate pyruvate and L-glutamate
Nicotiana sylvestris
0.077
-
partially purified enzyme, substrate prephenate and L-aspartate
Nicotiana sylvestris
0.118
-
partially purified enzyme, substrate oxaloacetate and L-aspartate
Nicotiana sylvestris
0.125
-
partially purified enzyme, substrate oxaloacetate and L-glutamate
Nicotiana sylvestris
0.156
-
partially purified enzyme, substrate 2-oxoglutarate and L-aspartate
Nicotiana sylvestris
0.157
-
partially purified enzyme, substrate prephenate and L-glutamate
Nicotiana sylvestris
0.251
-
partially purified enzyme, substrate 2-oxoglutarate and L-glutamate
Nicotiana sylvestris
Storage Stability
Storage Stability
Organism
in presence of pyridoxal 5'-phosphate, EDTA, glycerol, stable for at least one month
Nicotiana sylvestris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + L-aspartate
-
657635
Nicotiana sylvestris
L-glutamate + oxaloacetate
-
-
-
r
2-oxoglutarate + L-glutamate
-
657635
Nicotiana sylvestris
L-glutamate + 2-oxoglutarate
-
-
-
r
additional information
substrate specificity, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and indolepyruvate using cosubstrate L-glutamate, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and pyruvate using cosubstrate L-aspartate
657635
Nicotiana sylvestris
?
-
-
-
-
oxaloacetate + L-aspartate
-
657635
Nicotiana sylvestris
L-aspartate + oxaloacetate
-
-
-
r
oxaloacetate + L-glutamate
-
657635
Nicotiana sylvestris
L-aspartate + 2-oxoglutarate
-
-
-
r
prephenate + L-aspartate
-
657635
Nicotiana sylvestris
L-arogenate + oxaloacetate
-
-
-
r
prephenate + L-glutamate
important step in biosynthesis of L-tyrosine and L-phenylalanine
657635
Nicotiana sylvestris
L-arogenate + 2-oxoglutarate
-
-
-
r
prephenate + L-glutamate
optimal prephenate concentration is 0.5-1.0 mM
657635
Nicotiana sylvestris
L-arogenate + 2-oxoglutarate
-
-
-
r
pyruvate + L-glutamate
-
657635
Nicotiana sylvestris
L-alanine + 2-oxoglutarate
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Nicotiana sylvestris
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
70
-
Nicotiana sylvestris
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
stable, at least for 17 min
Nicotiana sylvestris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.25
-
-
Nicotiana sylvestris
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required, stabilizes the enzyme
Nicotiana sylvestris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required, stabilizes the enzyme
Nicotiana sylvestris
General Stability (protein specific)
General Stability
Organism
pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay
Nicotiana sylvestris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
prephenate
substrate inhibition above 1 mM, antagonism by L-glutamate
Nicotiana sylvestris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prephenate + L-glutamate
Nicotiana sylvestris
important step in biosynthesis of L-tyrosine and L-phenylalanine
L-arogenate + 2-oxoglutarate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
partially by ion exchange chromatography
Nicotiana sylvestris
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
stable cell line ANS-1
Nicotiana sylvestris
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
substrate specificity
Nicotiana sylvestris
0.042
-
crude enzyme extract, substrates prephenate and L-glutamate
Nicotiana sylvestris
0.073
-
partially purified enzyme, substrate pyruvate and L-glutamate
Nicotiana sylvestris
0.077
-
partially purified enzyme, substrate prephenate and L-aspartate
Nicotiana sylvestris
0.118
-
partially purified enzyme, substrate oxaloacetate and L-aspartate
Nicotiana sylvestris
0.125
-
partially purified enzyme, substrate oxaloacetate and L-glutamate
Nicotiana sylvestris
0.156
-
partially purified enzyme, substrate 2-oxoglutarate and L-aspartate
Nicotiana sylvestris
0.157
-
partially purified enzyme, substrate prephenate and L-glutamate
Nicotiana sylvestris
0.251
-
partially purified enzyme, substrate 2-oxoglutarate and L-glutamate
Nicotiana sylvestris
Storage Stability (protein specific)
Storage Stability
Organism
in presence of pyridoxal 5'-phosphate, EDTA, glycerol, stable for at least one month
Nicotiana sylvestris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + L-aspartate
-
657635
Nicotiana sylvestris
L-glutamate + oxaloacetate
-
-
-
r
2-oxoglutarate + L-glutamate
-
657635
Nicotiana sylvestris
L-glutamate + 2-oxoglutarate
-
-
-
r
additional information
substrate specificity, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and indolepyruvate using cosubstrate L-glutamate, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and pyruvate using cosubstrate L-aspartate
657635
Nicotiana sylvestris
?
-
-
-
-
oxaloacetate + L-aspartate
-
657635
Nicotiana sylvestris
L-aspartate + oxaloacetate
-
-
-
r
oxaloacetate + L-glutamate
-
657635
Nicotiana sylvestris
L-aspartate + 2-oxoglutarate
-
-
-
r
prephenate + L-aspartate
-
657635
Nicotiana sylvestris
L-arogenate + oxaloacetate
-
-
-
r
prephenate + L-glutamate
important step in biosynthesis of L-tyrosine and L-phenylalanine
657635
Nicotiana sylvestris
L-arogenate + 2-oxoglutarate
-
-
-
r
prephenate + L-glutamate
optimal prephenate concentration is 0.5-1.0 mM
657635
Nicotiana sylvestris
L-arogenate + 2-oxoglutarate
-
-
-
r
pyruvate + L-glutamate
-
657635
Nicotiana sylvestris
L-alanine + 2-oxoglutarate
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Nicotiana sylvestris
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
70
-
Nicotiana sylvestris
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
stable, at least for 17 min
Nicotiana sylvestris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.25
-
-
Nicotiana sylvestris
Other publictions for EC 2.6.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738632
Graindorge
Three different classes of ami ...
Sinorhizobium meliloti, Streptomyces avermitilis, Streptomyces avermitilis DSM 46492, Synechocystis sp.
J. Biol. Chem.
289
3198-3208
2014
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
739351
de la Torre
Deciphering the role of aspart ...
Nicotiana benthamiana
Plant Physiol.
164
92-104
2014
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722267
Graindorge
Identification of a plant gene ...
Arabidopsis thaliana
FEBS Lett.
584
4357-4360
2010
-
-
1
-
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
659858
Bonner
Prephenate aminotransferase ...
Brevibacterium flavum, Corynebacterium glutamicum, Hemerocallis hybrid cultivar, Lemna sp., Magnolia sp., Nicotiana sylvestris, Sorghum sp., Spinacia oleracea
Methods Enzymol.
142
479-487
1987
-
-
-
-
-
1
1
-
-
-
1
8
-
8
-
-
3
-
-
15
2
1
37
-
1
1
8
-
1
-
-
8
-
-
-
-
-
-
8
-
-
1
-
1
-
-
-
-
1
8
-
-
-
3
-
15
2
1
37
-
1
1
8
-
1
-
-
-
-
-
-
-
-
-
660563
Siehl
Tyrosine biosynthesis in Sorgh ...
Sorghum bicolor x Sorghum sudanesis
Z. Naturforsch. C
41
79-86
1986
-
-
-
-
-
1
-
3
-
-
-
1
-
4
-
-
1
-
-
1
1
-
6
-
1
-
1
-
1
1
-
1
-
1
-
-
-
-
1
-
-
1
-
-
-
3
-
-
-
1
-
-
-
1
-
1
1
-
6
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
657635
Bonner
Novel features of prephenate a ...
Nicotiana sylvestris
Arch. Biochem. Biophys.
238
237-246
1985
-
-
-
-
-
1
1
-
-
-
-
1
-
1
-
-
1
-
-
1
9
1
9
-
1
1
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
-
1
-
1
9
1
9
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-