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Literature summary for 2.6.1.77 extracted from
- Identification of novel thermostable taurine-pyruvate transaminase from Geobacillus thermodenitrificans for chiral amine synthesis (2016), Appl. Microbiol. Biotechnol., 100, 3101-3111.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | incubation th at 50°C for 8 h or at 60°C for 30 min increases activity by up to 2.5fold | Geobacillus thermodenitrificans subsp. thermodenitrificans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Geobacillus thermodenitrificans subsp. thermodenitrificans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modeling of activte site. The active site shows a special large pocket and substrate tunnel | Geobacillus thermodenitrificans subsp. thermodenitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.3 | - |
taurine | pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Geobacillus thermodenitrificans subsp. thermodenitrificans |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | increase in DMSO concentration up to 10% enhances activity by up to 135% | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| Methanol | 5%, fast loss of activity | Geobacillus thermodenitrificans subsp. thermodenitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans subsp. thermodenitrificans | A0A0S2VC96 | - |
- |
| Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 | A0A0S2VC96 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.26 | - |
substrate (S)-1-methylbenzylamine, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 0.52 | - |
substrate methanal, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 0.67 | - |
substrate propanal, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 0.68 | - |
substrate butanal, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 0.68 | - |
substrate ethanal, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 0.71 | - |
substrate glyoxylate, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 0.75 | - |
substrate glycolaldehyde, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 0.78 | - |
substrate hexanal, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 0.8 | - |
substrate pentanal, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 10.2 | - |
substrate taurine, pH 9.0, 37°C | Geobacillus thermodenitrificans subsp. thermodenitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-1-methylbenzylamine + pyruvate | no substrate: (R)-enantiomer | Geobacillus thermodenitrificans subsp. thermodenitrificans | acetophenone + L-alanine | - |
? | |
| (S)-1-methylbenzylamine + pyruvate | no substrate: (R)-enantiomer | Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 | acetophenone + L-alanine | - |
? | |
| additional information | the enzyme displays broad donor and acceptor ranges for unnatural substrates, with relatively good activity toward ketoses. Enzyme shows a unique mechanism of sulfonate/alpha-carboxylate recognition contributed by Arg163 and Gln160 | Geobacillus thermodenitrificans subsp. thermodenitrificans | ? | - |
? | |
| additional information | the enzyme displays broad donor and acceptor ranges for unnatural substrates, with relatively good activity toward ketoses. Enzyme shows a unique mechanism of sulfonate/alpha-carboxylate recognition contributed by Arg163 and Gln160 | Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 | ? | - |
? | |
| taurine + butanal | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + butyl-1-amine | - |
? | |
| taurine + butanal | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 | 2-sulfoacetaldehyde + butyl-1-amine | - |
? | |
| taurine + ethanal | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + ethylamine | - |
? | |
| taurine + ethanal | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 | 2-sulfoacetaldehyde + ethylamine | - |
? | |
| taurine + glycolaldehyde | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + ? | - |
? | |
| taurine + glyoxylate | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + glycine | - |
? | |
| taurine + hexanal | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + hexyl-1-amine | - |
? | |
| taurine + methanal | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + methylamine | - |
? | |
| taurine + pentanal | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + pentylamine | - |
? | |
| taurine + propanal | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + propyl-1-amine | - |
? | |
| taurine + pyruvate | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans | 2-sulfoacetaldehyde + L-alanine | - |
? | |
| taurine + pyruvate | - |
Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 | 2-sulfoacetaldehyde + L-alanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Geobacillus thermodenitrificans subsp. thermodenitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Geobacillus thermodenitrificans subsp. thermodenitrificans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
17% of maximum activity | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 70 | - |
less than 30% of maximum activity | Geobacillus thermodenitrificans subsp. thermodenitrificans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
8 h, 2.5fold increase in activity | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 60 | - |
30 min, 2.5fold increase in activity | Geobacillus thermodenitrificans subsp. thermodenitrificans |
| 70 | - |
15 min, almost complete loss of activity | Geobacillus thermodenitrificans subsp. thermodenitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Geobacillus thermodenitrificans subsp. thermodenitrificans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | more than 20% of maximum activity | Geobacillus thermodenitrificans subsp. thermodenitrificans |
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Release 2023.1 (January 2023)
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