Data extracted from this reference:
Application
synthesis
production of ectoine in Escherichia coli. The Escherichia coli regulatory protein AraC is engineered to recognize ectoine as it snon-natural effector and to activate transcription upon ectoine binding. The ectoine biosynthetic cluster from Halomonas elongata is cloned into Escherichia coli. By engineering the rate-limiting enzyme L-2,4-diaminobutyric acid aminotransferase EctB, ectoine production and the specific activityof the EctB mutant are increased
Halomonas elongata
Engineering
D180V
about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
D180V/E36V
double mutant displays less activity than mutant D180V
Halomonas elongata
D180V/F320Y/Q325R
about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
E36V
about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
KM Value [mM]
0.04
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
0.07
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
0.09
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
0.21
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
0.53
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
1.07
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
Organism
Halomonas elongata
O52250
CGMCC 1.6329
Halomonas elongata DSM 2581
O52250
CGMCC 1.6329
Substrates and Products (Substrate)
L-2,4-diaminobutanoate + 2-oxoglutarate
738983
Halomonas elongata
L-aspartate 4-semialdehyde + L-glutamate
?
L-2,4-diaminobutanoate + 2-oxoglutarate
738983
Halomonas elongata DSM 2581
L-aspartate 4-semialdehyde + L-glutamate
?
Turnover Number [1/s]
4.5
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
8.4
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
25.2
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
120
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
182
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
221
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
Application (protein specific)
synthesis
production of ectoine in Escherichia coli. The Escherichia coli regulatory protein AraC is engineered to recognize ectoine as it snon-natural effector and to activate transcription upon ectoine binding. The ectoine biosynthetic cluster from Halomonas elongata is cloned into Escherichia coli. By engineering the rate-limiting enzyme L-2,4-diaminobutyric acid aminotransferase EctB, ectoine production and the specific activityof the EctB mutant are increased
Halomonas elongata
Engineering (protein specific)
D180V
about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
D180V/E36V
double mutant displays less activity than mutant D180V
Halomonas elongata
D180V/F320Y/Q325R
about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
E36V
about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
KM Value [mM] (protein specific)
0.04
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
0.07
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
0.09
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
0.21
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
0.53
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
1.07
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
L-2,4-diaminobutanoate + 2-oxoglutarate
738983
Halomonas elongata
L-aspartate 4-semialdehyde + L-glutamate
?
L-2,4-diaminobutanoate + 2-oxoglutarate
738983
Halomonas elongata DSM 2581
L-aspartate 4-semialdehyde + L-glutamate
?
Turnover Number [1/s] (protein specific)
4.5
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
8.4
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
25.2
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
120
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
182
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
221
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
KCat/KM [mM/s]
4
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
16
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
120
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
1333
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
2601
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
5535
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
KCat/KM [mM/s] (protein specific)
4
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
16
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
120
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
1333
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
2601
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
5535
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
Other publictions for EC 2.6.1.76
738983
Chen
Design of an ectoine-responsiv ...
Halomonas elongata, Halomonas elongata DSM 2581
Metab. Eng.
30
149-155
2015
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1
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4
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6
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2
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6
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1
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4
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6
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2
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6
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6
6
640091
Ono
Characterization of biosynthet ...
Halomonas elongata
J. Bacteriol.
181
91-99
1999
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2
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1
2
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1
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1
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1
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1
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1
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6
1
1
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1
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1
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288732
Ikai
Two genes involved in the 1,3- ...
Acinetobacter baumannii, Haemophilus influenzae
Biol. Pharm. Bull.
21
170-173
1998
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2
1
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6
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288731
Ikai
Identification and analysis of ...
Acinetobacter baumannii, Acinetobacter calcoaceticus, Acinetobacter genospecies, Acinetobacter haemolyticus, Acinetobacter johnsonii, Acinetobacter junii, Acinetobacter lwoffii, Acinetobacter radioresistens, Acinetobacter radioresistens IAM13186
J. Bacteriol.
179
5118-5125
1997
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8
8
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2
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3
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12
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1
1
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21
1
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1
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8
8
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1
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21
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