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Literature summary for 2.6.1.76 extracted from

  • Chen, W.; Zhang, S.; Jiang, P.; Yao, J.; He, Y.; Chen, L.; Gui, X.; Dong, Z.; Tang, S.Y.
    Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis (2015), Metab. Eng., 30, 149-155.
    View publication on PubMed
Search for more literature for 2.6.1.76

Application

Application Comment Organism
synthesis production of ectoine in Escherichia coli. The Escherichia coli regulatory protein AraC is engineered to recognize ectoine as it snon-natural effector and to activate transcription upon ectoine binding. The ectoine biosynthetic cluster from Halomonas elongata is cloned into Escherichia coli. By engineering the rate-limiting enzyme L-2,4-diaminobutyric acid aminotransferase EctB, ectoine production and the specific activityof the EctB mutant are increased Halomonas elongata

Protein Variants

Protein Variants Comment Organism
D180V about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant Halomonas elongata
D180V/E36V double mutant displays less activity than mutant D180V Halomonas elongata
D180V/F320Y/Q325R about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant Halomonas elongata
E36V about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant Halomonas elongata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 L-2,4-diaminobutanoate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata
0.07
-
 L-2,4-diaminobutanoate mutant D180V, pH 8.5, 37°C Halomonas elongata
0.09
-
 L-2,4-diaminobutanoate wild-type, pH 8.5, 37°C Halomonas elongata
0.21
-
 2-oxoglutarate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata
0.53
-
 2-oxoglutarate mutant D180V, pH 8.5, 37°C Halomonas elongata
1.07
-
 2-oxoglutarate wild-type, pH 8.5, 37°C Halomonas elongata

Organism

Organism UniProt Comment Textmining
Halomonas elongata O52250 CGMCC 1.6329
-
Halomonas elongata DSM 2581 O52250 CGMCC 1.6329
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2,4-diaminobutanoate + 2-oxoglutarate
-
 Halomonas elongata L-aspartate 4-semialdehyde + L-glutamate
-
 ?
L-2,4-diaminobutanoate + 2-oxoglutarate
-
 Halomonas elongata DSM 2581 L-aspartate 4-semialdehyde + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
EctB
-
 Halomonas elongata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5
-
 2-oxoglutarate wild-type, pH 8.5, 37°C Halomonas elongata
8.4
-
 2-oxoglutarate mutant D180V, pH 8.5, 37°C Halomonas elongata
25.2
-
 2-oxoglutarate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata
120
-
 L-2,4-diaminobutanoate wild-type, pH 8.5, 37°C Halomonas elongata
182
-
 L-2,4-diaminobutanoate mutant D180V, pH 8.5, 37°C Halomonas elongata
221
-
 L-2,4-diaminobutanoate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
 2-oxoglutarate wild-type, pH 8.5, 37°C Halomonas elongata
16
-
 2-oxoglutarate mutant D180V, pH 8.5, 37°C Halomonas elongata
120
-
 2-oxoglutarate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata
1333
-
 L-2,4-diaminobutanoate wild-type, pH 8.5, 37°C Halomonas elongata
2601
-
 L-2,4-diaminobutanoate mutant D180V, pH 8.5, 37°C Halomonas elongata
5535
-
 L-2,4-diaminobutanoate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata