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show all sequences of 2.6.1.76

Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis

Chen, W.; Zhang, S.; Jiang, P.; Yao, J.; He, Y.; Chen, L.; Gui, X.; Dong, Z.; Tang, S.Y.; Metab. Eng. 30, 149-155 (2015)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
production of ectoine in Escherichia coli. The Escherichia coli regulatory protein AraC is engineered to recognize ectoine as it snon-natural effector and to activate transcription upon ectoine binding. The ectoine biosynthetic cluster from Halomonas elongata is cloned into Escherichia coli. By engineering the rate-limiting enzyme L-2,4-diaminobutyric acid aminotransferase EctB, ectoine production and the specific activityof the EctB mutant are increased
Halomonas elongata
Engineering
Amino acid exchange
Commentary
Organism
D180V
about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
D180V/E36V
double mutant displays less activity than mutant D180V
Halomonas elongata
D180V/F320Y/Q325R
about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
E36V
about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
0.07
-
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
0.09
-
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
0.21
-
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
0.53
-
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
1.07
-
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halomonas elongata
O52250
CGMCC 1.6329
-
Halomonas elongata DSM 2581
O52250
CGMCC 1.6329
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-2,4-diaminobutanoate + 2-oxoglutarate
-
738983
Halomonas elongata
L-aspartate 4-semialdehyde + L-glutamate
-
-
-
?
L-2,4-diaminobutanoate + 2-oxoglutarate
-
738983
Halomonas elongata DSM 2581
L-aspartate 4-semialdehyde + L-glutamate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.5
-
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
8.4
-
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
25.2
-
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
120
-
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
182
-
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
221
-
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
Application (protein specific)
Application
Commentary
Organism
synthesis
production of ectoine in Escherichia coli. The Escherichia coli regulatory protein AraC is engineered to recognize ectoine as it snon-natural effector and to activate transcription upon ectoine binding. The ectoine biosynthetic cluster from Halomonas elongata is cloned into Escherichia coli. By engineering the rate-limiting enzyme L-2,4-diaminobutyric acid aminotransferase EctB, ectoine production and the specific activityof the EctB mutant are increased
Halomonas elongata
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D180V
about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
D180V/E36V
double mutant displays less activity than mutant D180V
Halomonas elongata
D180V/F320Y/Q325R
about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
E36V
about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant
Halomonas elongata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
0.07
-
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
0.09
-
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
0.21
-
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
0.53
-
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
1.07
-
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-2,4-diaminobutanoate + 2-oxoglutarate
-
738983
Halomonas elongata
L-aspartate 4-semialdehyde + L-glutamate
-
-
-
?
L-2,4-diaminobutanoate + 2-oxoglutarate
-
738983
Halomonas elongata DSM 2581
L-aspartate 4-semialdehyde + L-glutamate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.5
-
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
8.4
-
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
25.2
-
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
120
-
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
182
-
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
221
-
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4
-
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
16
-
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
120
-
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
1333
-
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
2601
-
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
5535
-
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4
-
2-oxoglutarate
wild-type, pH 8.5, 37°C
Halomonas elongata
16
-
2-oxoglutarate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
120
-
2-oxoglutarate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
1333
-
L-2,4-diaminobutanoate
wild-type, pH 8.5, 37°C
Halomonas elongata
2601
-
L-2,4-diaminobutanoate
mutant D180V, pH 8.5, 37°C
Halomonas elongata
5535
-
L-2,4-diaminobutanoate
mutant D180V/F320Y/Q325R , pH 8.5, 37°C
Halomonas elongata
Other publictions for EC 2.6.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738983
Chen
Design of an ectoine-responsiv ...
Halomonas elongata, Halomonas elongata DSM 2581
Metab. Eng.
30
149-155
2015
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6
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640091
Ono
Characterization of biosynthet ...
Halomonas elongata
J. Bacteriol.
181
91-99
1999
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2
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1
2
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1
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1
1
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1
1
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1
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1
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288732
Ikai
Two genes involved in the 1,3- ...
Acinetobacter baumannii, Haemophilus influenzae
Biol. Pharm. Bull.
21
170-173
1998
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2
1
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288731
Ikai
Identification and analysis of ...
Acinetobacter baumannii, Acinetobacter calcoaceticus, Acinetobacter genospecies, Acinetobacter haemolyticus, Acinetobacter johnsonii, Acinetobacter junii, Acinetobacter lwoffii, Acinetobacter radioresistens, Acinetobacter radioresistens IAM13186
J. Bacteriol.
179
5118-5125
1997
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8
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3
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12
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1
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