BRENDA - Reference to 2.6.1.76; Id = 738983

synthesis

production of ectoine in Escherichia coli. The Escherichia coli regulatory protein AraC is engineered to recognize ectoine as it snon-natural effector and to activate transcription upon ectoine binding. The ectoine biosynthetic cluster from Halomonas elongata is cloned into Escherichia coli. By engineering the rate-limiting enzyme L-2,4-diaminobutyric acid aminotransferase EctB, ectoine production and the specific activityof the EctB mutant are increased

Halomonas elongata

D180V

about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant

Halomonas elongata

D180V/E36V

double mutant displays less activity than mutant D180V

Halomonas elongata

D180V/F320Y/Q325R

about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant

Halomonas elongata

E36V

about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant

Halomonas elongata

0.04

-

L-2,4-diaminobutanoate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

0.07

-

L-2,4-diaminobutanoate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

0.09

-

L-2,4-diaminobutanoate

wild-type, pH 8.5, 37°C

Halomonas elongata

0.21

-

2-oxoglutarate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

0.53

-

2-oxoglutarate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

1.07

-

2-oxoglutarate

wild-type, pH 8.5, 37°C

Halomonas elongata

O52250

CGMCC 1.6329

-

Halomonas elongata DSM 2581

O52250

CGMCC 1.6329

-

L-2,4-diaminobutanoate + 2-oxoglutarate

-

738983

Halomonas elongata

L-aspartate 4-semialdehyde + L-glutamate

-

?

L-2,4-diaminobutanoate + 2-oxoglutarate

-

738983

Halomonas elongata DSM 2581

L-aspartate 4-semialdehyde + L-glutamate

-

?

4.5

-

2-oxoglutarate

wild-type, pH 8.5, 37°C

Halomonas elongata

8.4

-

2-oxoglutarate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

25.2

-

2-oxoglutarate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

120

-

L-2,4-diaminobutanoate

wild-type, pH 8.5, 37°C

Halomonas elongata

182

-

L-2,4-diaminobutanoate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

221

-

L-2,4-diaminobutanoate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

synthesis

production of ectoine in Escherichia coli. The Escherichia coli regulatory protein AraC is engineered to recognize ectoine as it snon-natural effector and to activate transcription upon ectoine binding. The ectoine biosynthetic cluster from Halomonas elongata is cloned into Escherichia coli. By engineering the rate-limiting enzyme L-2,4-diaminobutyric acid aminotransferase EctB, ectoine production and the specific activityof the EctB mutant are increased

Halomonas elongata

D180V

about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant

Halomonas elongata

D180V/E36V

double mutant displays less activity than mutant D180V

Halomonas elongata

D180V/F320Y/Q325R

about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant

Halomonas elongata

E36V

about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant

Halomonas elongata

0.04

-

L-2,4-diaminobutanoate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

0.07

-

L-2,4-diaminobutanoate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

0.09

-

L-2,4-diaminobutanoate

wild-type, pH 8.5, 37°C

Halomonas elongata

0.21

-

2-oxoglutarate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

0.53

-

2-oxoglutarate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

1.07

-

2-oxoglutarate

wild-type, pH 8.5, 37°C

Halomonas elongata

L-2,4-diaminobutanoate + 2-oxoglutarate

-

738983

Halomonas elongata

L-aspartate 4-semialdehyde + L-glutamate

-

?

L-2,4-diaminobutanoate + 2-oxoglutarate

-

738983

Halomonas elongata DSM 2581

L-aspartate 4-semialdehyde + L-glutamate

-

?

4.5

-

2-oxoglutarate

wild-type, pH 8.5, 37°C

Halomonas elongata

8.4

-

2-oxoglutarate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

25.2

-

2-oxoglutarate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

120

-

L-2,4-diaminobutanoate

wild-type, pH 8.5, 37°C

Halomonas elongata

182

-

L-2,4-diaminobutanoate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

221

-

L-2,4-diaminobutanoate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

4

-

2-oxoglutarate

wild-type, pH 8.5, 37°C

Halomonas elongata

16

-

2-oxoglutarate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

120

-

2-oxoglutarate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

1333

-

L-2,4-diaminobutanoate

wild-type, pH 8.5, 37°C

Halomonas elongata

2601

-

L-2,4-diaminobutanoate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

5535

-

L-2,4-diaminobutanoate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

4

-

2-oxoglutarate

wild-type, pH 8.5, 37°C

Halomonas elongata

16

-

2-oxoglutarate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

120

-

2-oxoglutarate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

1333

-

L-2,4-diaminobutanoate

wild-type, pH 8.5, 37°C

Halomonas elongata

2601

-

L-2,4-diaminobutanoate

mutant D180V, pH 8.5, 37°C

Halomonas elongata

5535

-

L-2,4-diaminobutanoate

mutant D180V/F320Y/Q325R , pH 8.5, 37°C

Halomonas elongata

738983

Chen

Design of an ectoine-responsiv ...

Halomonas elongata, Halomonas elongata DSM 2581

Metab. Eng.

30

149-155

2015

-

1

-

4

-

6

-

2

-

2

-

6

-

1

-

4

-

6

-

2

-

6

-

6

640091

Ono

Characterization of biosynthet ...

Halomonas elongata

J. Bacteriol.

181

91-99

1999

-

2

-

1

2

-

1

-

1

-

1

-

6

1

-

1

-

1

-

1

-

1

-

2

-

1

2

-

1

-

1

-

6

1

-

1

-

1

-

288732

Ikai

Two genes involved in the 1,3- ...

Acinetobacter baumannii, Haemophilus influenzae

Biol. Pharm. Bull.

21

170-173

1998

-

2

1

-

2

-

6

-

1

-

4

-

2

1

-

2

-

4

-

288731

Ikai

Identification and analysis of ...

Acinetobacter baumannii, Acinetobacter calcoaceticus, Acinetobacter genospecies, Acinetobacter haemolyticus, Acinetobacter johnsonii, Acinetobacter junii, Acinetobacter lwoffii, Acinetobacter radioresistens, Acinetobacter radioresistens IAM13186

J. Bacteriol.

179

5118-5125

1997

-

8

-

2

-

3

-

12

-

1

-

21

1

-

1

-

8

-

2

-

3

-

1

-

21

1

-

1

-

Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis

Data extracted from this reference: