BRENDA - Enzyme Database show
show all sequences of 2.6.1.76

Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata

Ono, H.; Sawada, K.; Khunajakr, N.; Tao, T.; Yamamoto, M.; Hiramoto, M.; Shinmyo, A.; Takano, M.; Murooka, Y.; J. Bacteriol. 181, 91-99 (1999)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.5
-
L-aspartate 4-semialdehyde
pH 8.5, 25C
Halomonas elongata
9.1
-
L-glutamate
pH 8.5, 25C
Halomonas elongata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
required
Halomonas elongata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
6 * 44000, SDS-PAGE
Halomonas elongata
260000
-
gel filtration
Halomonas elongata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halomonas elongata
O52250
-
-
Purification (Commentary)
Commentary
Organism
-
Halomonas elongata
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
12
-
pH 8.5, 25C
Halomonas elongata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutanoate + 2-oxoglutarate
about 60% of the activity with L-2,4-diaminobutanoate
640091
Halomonas elongata
? + L-glutamate
-
-
-
r
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640091
Halomonas elongata
L-aspartate 4-semialdehyde + L-glutamate
-
-
-
r
L-aspartate 4-semialdehyde + L-glutamate
-
640091
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
-
-
r
L-aspartate 4-semialdehyde + L-homoserine
about 5% of the activity with L-glutamate
640091
Halomonas elongata
L-2,4-diaminobutanoate + 4-hydroxy-2-oxobutanoic acid
-
-
-
r
L-ornithine + 2-oxoglutarate
about 6% of the activity with L-2,4-diaminobutanoate
640091
Halomonas elongata
? + L-glutamate
-
-
-
r
additional information
no substrates: L-alanine, L-aspartate, L-lysine
640091
Halomonas elongata
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
hexamer
6 * 44000, SDS-PAGE
Halomonas elongata
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Halomonas elongata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
-
Halomonas elongata
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Halomonas elongata
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Halomonas elongata
isoelectric focussing
-
6.2
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Halomonas elongata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.5
-
L-aspartate 4-semialdehyde
pH 8.5, 25C
Halomonas elongata
9.1
-
L-glutamate
pH 8.5, 25C
Halomonas elongata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
required
Halomonas elongata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
6 * 44000, SDS-PAGE
Halomonas elongata
260000
-
gel filtration
Halomonas elongata
Purification (Commentary) (protein specific)
Commentary
Organism
-
Halomonas elongata
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
12
-
pH 8.5, 25C
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutanoate + 2-oxoglutarate
about 60% of the activity with L-2,4-diaminobutanoate
640091
Halomonas elongata
? + L-glutamate
-
-
-
r
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640091
Halomonas elongata
L-aspartate 4-semialdehyde + L-glutamate
-
-
-
r
L-aspartate 4-semialdehyde + L-glutamate
-
640091
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
-
-
r
L-aspartate 4-semialdehyde + L-homoserine
about 5% of the activity with L-glutamate
640091
Halomonas elongata
L-2,4-diaminobutanoate + 4-hydroxy-2-oxobutanoic acid
-
-
-
r
L-ornithine + 2-oxoglutarate
about 6% of the activity with L-2,4-diaminobutanoate
640091
Halomonas elongata
? + L-glutamate
-
-
-
r
additional information
no substrates: L-alanine, L-aspartate, L-lysine
640091
Halomonas elongata
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 44000, SDS-PAGE
Halomonas elongata
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Halomonas elongata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
-
Halomonas elongata
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Halomonas elongata
isoelectric focussing
-
6.2
Other publictions for EC 2.6.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738983
Chen
Design of an ectoine-responsiv ...
Halomonas elongata, Halomonas elongata DSM 2581
Metab. Eng.
30
149-155
2015
-
1
-
-
4
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
640091
Ono
Characterization of biosynthet ...
Halomonas elongata
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
-
-
2
-
1
2
-
-
1
-
-
1
-
-
-
1
-
6
1
1
-
-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
2
-
1
2
-
-
-
-
1
-
-
1
-
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
288732
Ikai
Two genes involved in the 1,3- ...
Acinetobacter baumannii, Haemophilus influenzae
Biol. Pharm. Bull.
21
170-173
1998
-
2
1
-
-
-
-
-
-
-
2
-
-
6
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288731
Ikai
Identification and analysis of ...
Acinetobacter baumannii, Acinetobacter calcoaceticus, Acinetobacter genospecies, Acinetobacter haemolyticus, Acinetobacter johnsonii, Acinetobacter junii, Acinetobacter lwoffii, Acinetobacter radioresistens, Acinetobacter radioresistens IAM13186
J. Bacteriol.
179
5118-5125
1997
-
8
8
-
-
-
-
2
-
-
3
-
-
12
-
-
1
1
-
-
-
-
21
1
-
-
-
-
1
-
-
-
-
-
-
-
8
8
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
1
-
-
-
-
21
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-