BRENDA - Enzyme Database
show all sequences of 2.6.1.72

Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Jariyachawalid, K.; Laowanapiban, P.; Meevootisom, V.; Wiyakrutta, S.; Microb. Cell Fact. 11, 47 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
functional expression in Pichia pastoris. Co-expression of Escherichia coli chaperonins GroEL-GroES with the enzyme gene dramatically improves the soluble active enzyme production. Increasing gene dosage of both the enzyme and those of the chaperones further increases functional protein yield up to 14400fold higher than when the enzyme is expressed alone. Optimization of cultivation condition further increases activity yield from the best co-expressing strain by 1.2fold
Pseudomonas stutzeri
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas stutzeri
Q6VY99
-
-
Pseudomonas stutzeri ST201
Q6VY99
-
-
Application (protein specific)
Application
Commentary
Organism
synthesis
functional expression in Pichia pastoris. Co-expression of Escherichia coli chaperonins GroEL-GroES with the enzyme gene dramatically improves the soluble active enzyme production. Increasing gene dosage of both the enzyme and those of the chaperones further increases functional protein yield up to 14400fold higher than when the enzyme is expressed alone. Optimization of cultivation condition further increases activity yield from the best co-expressing strain by 1.2fold
Pseudomonas stutzeri
Other publictions for EC 2.6.1.72
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738859
Javid
Effects of halophilic peptide ...
Pseudomonas stutzeri, Pseudomonas stutzeri ST-201
J. Microbiol. Biotechnol.
24
597-604
2014
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1
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5
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723112
Jariyachawalid
Effective enhancement of Pseud ...
Pseudomonas stutzeri, Pseudomonas stutzeri ST201
Microb. Cell Fact.
11
47
2012
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1
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8
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1
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675793
Mller
Metabolic engineering of the E ...
Pseudomonas putida
Metab. Eng.
8
196-208
2006
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1
1
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1
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5
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1
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1
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636991
Van den Tweel
-
DL-4-Hydroxyphenylglycine cata ...
Pseudomonas putida, Pseudomonas putida LW-4, Pseudomonas putida MW 27
Arch. Microbiol.
150
471-476
1988
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3
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5
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1
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1
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9
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1
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1
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1
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3
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1
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1
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9
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1
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636992
Van den Tweel
-
The involvement of an enantios ...
Pseudomonas putida, Pseudomonas putida LW-4
Appl. Microbiol. Biotechnol.
29
224-230
1988
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1
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2
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4
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1
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6
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1
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1
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1
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1
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1
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2
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1
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6
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1
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1
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