BRENDA - Enzyme Database
show all sequences of 2.6.1.72

Metabolic engineering of the E. coli L-phenylalanine pathway for the production of D-phenylglycine (D-Phg)

Müller, U.; van Assema, F.; Gunsior, M.; Orf, S.; Kremer, S.; Schipper, D.; Wagemans, A.; Townsend, C.A.; Sonke, T.; Bovenberg, R.; Wubbolts, M.; Metab. Eng. 8, 196-208 (2006)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
metabolic engineering of the Escherichia coli L-phenylalanine pathway for the production of D-phenylglycine. Expression of hydroxymandelate synthase from Amycolatopsis orientalis, hydroxymandelate oxidase from Streptomyces coelicolor and hydroxyphenylglycine aminotransferase from Pseudomonas putida in Escherichia coli
Pseudomonas putida
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxyphenylglyoxylate + L-glutamate
Pseudomonas putida
enzyme is involved in biosynthesis of D-4-hydroxyphenylglycine
D-4-hydroxyphenylglycine + 2-oxoglutarate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxyphenylglyoxylate + L-glutamate
enzyme is involved in biosynthesis of D-4-hydroxyphenylglycine
675793
Pseudomonas putida
D-4-hydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
metabolic engineering of the Escherichia coli L-phenylalanine pathway for the production of D-phenylglycine. Expression of hydroxymandelate synthase from Amycolatopsis orientalis, hydroxymandelate oxidase from Streptomyces coelicolor and hydroxyphenylglycine aminotransferase from Pseudomonas putida in Escherichia coli
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxyphenylglyoxylate + L-glutamate
Pseudomonas putida
enzyme is involved in biosynthesis of D-4-hydroxyphenylglycine
D-4-hydroxyphenylglycine + 2-oxoglutarate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxyphenylglyoxylate + L-glutamate
enzyme is involved in biosynthesis of D-4-hydroxyphenylglycine
675793
Pseudomonas putida
D-4-hydroxyphenylglycine + 2-oxoglutarate
-
-
-
?
Other publictions for EC 2.6.1.72
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738859
Javid
Effects of halophilic peptide ...
Pseudomonas stutzeri, Pseudomonas stutzeri ST-201
J. Microbiol. Biotechnol.
24
597-604
2014
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723112
Jariyachawalid
Effective enhancement of Pseud ...
Pseudomonas stutzeri, Pseudomonas stutzeri ST201
Microb. Cell Fact.
11
47
2012
-
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675793
Müller
Metabolic engineering of the E ...
Pseudomonas putida
Metab. Eng.
8
196-208
2006
-
1
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636991
Van den Tweel
-
DL-4-Hydroxyphenylglycine cata ...
Pseudomonas putida, Pseudomonas putida LW-4, Pseudomonas putida MW 27
Arch. Microbiol.
150
471-476
1988
-
-
-
-
-
-
-
-
-
-
-
3
-
5
-
-
1
-
-
-
1
-
9
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
1
-
9
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
636992
Van den Tweel
-
The involvement of an enantios ...
Pseudomonas putida, Pseudomonas putida LW-4
Appl. Microbiol. Biotechnol.
29
224-230
1988
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
1
-
-
-
-
-
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-