BRENDA - Enzyme Database show
show all sequences of 2.6.1.71

A novel enzyme, L-lysine:pyruvate aminotransferase, catalyses the first step of lysine catabolism in Pichia guilliermondii

Schmidt, H.; Bode, R.; Birnbaum, D.; FEMS Microbiol. Lett. 49, 203-206 (1988)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
gel filtration
Meyerozyma guilliermondii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-lysine + pyruvate
Meyerozyma guilliermondii
-
2-aminoadipate 6-semialdehyde + L-alanine
-
Meyerozyma guilliermondii
?
L-lysine + pyruvate
Meyerozyma guilliermondii H17
-
2-aminoadipate 6-semialdehyde + L-alanine
-
Meyerozyma guilliermondii H17
?
additional information
Meyerozyma guilliermondii
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
?
-
-
-
additional information
Meyerozyma guilliermondii H17
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Meyerozyma guilliermondii
-
-
-
Meyerozyma guilliermondii H17
-
-
-
Purification (Commentary)
Commentary
Organism
centrifugation and DEAE-cellulose, partial purification
Meyerozyma guilliermondii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-lysine + pyruvate
-
640194
Meyerozyma guilliermondii
2-aminoadipate 6-semialdehyde + L-alanine
-
640194
Meyerozyma guilliermondii
?
L-lysine + pyruvate
-
640194
Meyerozyma guilliermondii H17
2-aminoadipate 6-semialdehyde + L-alanine
-
640194
Meyerozyma guilliermondii H17
?
additional information
D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates
640194
Meyerozyma guilliermondii
?
-
-
-
-
additional information
rate of reaction strongly increased in the presence of pyridoxal 5'-phosphate
640194
Meyerozyma guilliermondii
?
-
-
-
-
additional information
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
640194
Meyerozyma guilliermondii
?
-
-
-
-
additional information
D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates
640194
Meyerozyma guilliermondii H17
?
-
-
-
-
additional information
rate of reaction strongly increased in the presence of pyridoxal 5'-phosphate
640194
Meyerozyma guilliermondii H17
?
-
-
-
-
additional information
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
640194
Meyerozyma guilliermondii H17
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Meyerozyma guilliermondii
32
-
-
Meyerozyma guilliermondii
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
increases activity; Km: 0.04 mM
Meyerozyma guilliermondii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
increases activity; Km: 0.04 mM
Meyerozyma guilliermondii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
gel filtration
Meyerozyma guilliermondii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-lysine + pyruvate
Meyerozyma guilliermondii
-
2-aminoadipate 6-semialdehyde + L-alanine
-
Meyerozyma guilliermondii
?
L-lysine + pyruvate
Meyerozyma guilliermondii H17
-
2-aminoadipate 6-semialdehyde + L-alanine
-
Meyerozyma guilliermondii H17
?
additional information
Meyerozyma guilliermondii
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
?
-
-
-
additional information
Meyerozyma guilliermondii H17
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
centrifugation and DEAE-cellulose, partial purification
Meyerozyma guilliermondii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-lysine + pyruvate
-
640194
Meyerozyma guilliermondii
2-aminoadipate 6-semialdehyde + L-alanine
-
640194
Meyerozyma guilliermondii
?
L-lysine + pyruvate
-
640194
Meyerozyma guilliermondii H17
2-aminoadipate 6-semialdehyde + L-alanine
-
640194
Meyerozyma guilliermondii H17
?
additional information
D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates
640194
Meyerozyma guilliermondii
?
-
-
-
-
additional information
rate of reaction strongly increased in the presence of pyridoxal 5'-phosphate
640194
Meyerozyma guilliermondii
?
-
-
-
-
additional information
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
640194
Meyerozyma guilliermondii
?
-
-
-
-
additional information
D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates
640194
Meyerozyma guilliermondii H17
?
-
-
-
-
additional information
rate of reaction strongly increased in the presence of pyridoxal 5'-phosphate
640194
Meyerozyma guilliermondii H17
?
-
-
-
-
additional information
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
640194
Meyerozyma guilliermondii H17
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Meyerozyma guilliermondii
32
-
-
Meyerozyma guilliermondii
Other publictions for EC 2.6.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722527
Chou
L-lysine catabolism is control ...
Pseudomonas aeruginosa
J. Bacteriol.
192
5874-5880
2010
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1
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1
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1
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1
1
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640194
Schmidt
A novel enzyme, L-lysine:pyruv ...
Meyerozyma guilliermondii, Meyerozyma guilliermondii H17
FEMS Microbiol. Lett.
49
203-206
1988
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1
4
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4
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1
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8
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2
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1
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1
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1
4
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1
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8
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2
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640193
Schmidt
-
Lysine degradation in Pichia g ...
Meyerozyma guilliermondii, Meyerozyma guilliermondii H17
J. Basic Microbiol.
27
595-601
1987
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12
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1
4
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4
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1
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1
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10
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2
2
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1
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1
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12
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1
4
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1
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1
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10
-
2
2
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