BRENDA - Enzyme Database show
show all sequences of 2.6.1.71

Lysine degradation in Pichia guilliermondii: characterisation of a novel enzyme, L-lysine:pyruvate aminotransferase

Schmidt, H.; Bode, R.; Birnbaum, D.; J. Basic Microbiol. 27, 595-601 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CaCl2
80% remaining activity at 1 mM, 7% remaining activity at 10 mM
Meyerozyma guilliermondii
CoCl2
9% activity retained at 1 mM, complete inhibition at 10 mM
Meyerozyma guilliermondii
CuCl2
complete inhibition at 1 mM or higher
Meyerozyma guilliermondii
EDTA
89% activity retained at 1 mM, 36% activity retained at 10 mM
Meyerozyma guilliermondii
FeCl2
23% activity retained at 1 mM, 13% activity retained at 10 mM
Meyerozyma guilliermondii
HgCl2
40% activity retained at 1 mM, 31% activity retained at 10 mM
Meyerozyma guilliermondii
hydroxylamine
37% activity retained at 1 mM, 6% activity retained at 10 mM
Meyerozyma guilliermondii
MgCl2
98% activity retained at 1 mM, 22% activity retained at 10 mM
Meyerozyma guilliermondii
MnCl2
57% activity retained at 1 mM, 3% activity retained at 10 mM
Meyerozyma guilliermondii
additional information
KF, KCN, sodium azide, Na2HAsSO4, NaHSO4, 2-mercaptoethanol, dithioerythritol, reduced glutathione, proteinogenic amino acids, none of them has inhibitory effects even at 10 mM
Meyerozyma guilliermondii
p-chloromercuribenzoate
5% activity retained at 1 mM, 1% activity retained at 10 mM
Meyerozyma guilliermondii
SnCl2
97% activity retained at 1 mM, 40% retained activity at 10 mM
Meyerozyma guilliermondii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
gel filtration
Meyerozyma guilliermondii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-lysine + pyruvate
Meyerozyma guilliermondii
involved in lysine degradation
2-aminoadipate 6-semialdehyde + L-alanine
-
Meyerozyma guilliermondii
?
L-lysine + pyruvate
Meyerozyma guilliermondii H17
involved in lysine degradation
2-aminoadipate 6-semialdehyde + L-alanine
-
Meyerozyma guilliermondii H17
?
additional information
Meyerozyma guilliermondii
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
?
-
-
-
additional information
Meyerozyma guilliermondii H17
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Meyerozyma guilliermondii
-
-
-
Meyerozyma guilliermondii H17
-
-
-
Purification (Commentary)
Commentary
Organism
centrifugation and DEAE-cellulose, partial purification
Meyerozyma guilliermondii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4
-
-
Meyerozyma guilliermondii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-amino-2-oxobutyrate + pyruvate
at 2% activity compared to L-lysine
640193
Meyerozyma guilliermondii
2,4-dioxobutanoate + L-alanine
-
-
-
?
4-amino-2-oxobutyrate + pyruvate
at 2% activity compared to L-lysine
640193
Meyerozyma guilliermondii H17
2,4-dioxobutanoate + L-alanine
-
-
-
?
L-lysine + pyruvate
-
640193
Meyerozyma guilliermondii
2-aminoadipate 6-semialdehyde + L-alanine
-
640193
Meyerozyma guilliermondii
?
L-lysine + pyruvate
involved in lysine degradation
640193
Meyerozyma guilliermondii
2-aminoadipate 6-semialdehyde + L-alanine
-
640193
Meyerozyma guilliermondii
?
L-lysine + pyruvate
-
640193
Meyerozyma guilliermondii H17
2-aminoadipate 6-semialdehyde + L-alanine
-
640193
Meyerozyma guilliermondii H17
?
L-lysine + pyruvate
involved in lysine degradation
640193
Meyerozyma guilliermondii H17
2-aminoadipate 6-semialdehyde + L-alanine
-
640193
Meyerozyma guilliermondii H17
?
additional information
D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates
640193
Meyerozyma guilliermondii
?
-
-
-
-
additional information
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
640193
Meyerozyma guilliermondii
?
-
-
-
-
additional information
D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates
640193
Meyerozyma guilliermondii H17
?
-
-
-
-
additional information
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
640193
Meyerozyma guilliermondii H17
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Meyerozyma guilliermondii
32
-
-
Meyerozyma guilliermondii
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
30
activity increases linearly with temperature
Meyerozyma guilliermondii
15
45
50% of maximal activity at 15°C and 45°C
Meyerozyma guilliermondii
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
Km: 0.04 mM
Meyerozyma guilliermondii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
Km: 0.04 mM
Meyerozyma guilliermondii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CaCl2
80% remaining activity at 1 mM, 7% remaining activity at 10 mM
Meyerozyma guilliermondii
CoCl2
9% activity retained at 1 mM, complete inhibition at 10 mM
Meyerozyma guilliermondii
CuCl2
complete inhibition at 1 mM or higher
Meyerozyma guilliermondii
EDTA
89% activity retained at 1 mM, 36% activity retained at 10 mM
Meyerozyma guilliermondii
FeCl2
23% activity retained at 1 mM, 13% activity retained at 10 mM
Meyerozyma guilliermondii
HgCl2
40% activity retained at 1 mM, 31% activity retained at 10 mM
Meyerozyma guilliermondii
hydroxylamine
37% activity retained at 1 mM, 6% activity retained at 10 mM
Meyerozyma guilliermondii
MgCl2
98% activity retained at 1 mM, 22% activity retained at 10 mM
Meyerozyma guilliermondii
MnCl2
57% activity retained at 1 mM, 3% activity retained at 10 mM
Meyerozyma guilliermondii
additional information
KF, KCN, sodium azide, Na2HAsSO4, NaHSO4, 2-mercaptoethanol, dithioerythritol, reduced glutathione, proteinogenic amino acids, none of them has inhibitory effects even at 10 mM
Meyerozyma guilliermondii
p-chloromercuribenzoate
5% activity retained at 1 mM, 1% activity retained at 10 mM
Meyerozyma guilliermondii
SnCl2
97% activity retained at 1 mM, 40% retained activity at 10 mM
Meyerozyma guilliermondii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90000
-
gel filtration
Meyerozyma guilliermondii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-lysine + pyruvate
Meyerozyma guilliermondii
involved in lysine degradation
2-aminoadipate 6-semialdehyde + L-alanine
-
Meyerozyma guilliermondii
?
L-lysine + pyruvate
Meyerozyma guilliermondii H17
involved in lysine degradation
2-aminoadipate 6-semialdehyde + L-alanine
-
Meyerozyma guilliermondii H17
?
additional information
Meyerozyma guilliermondii
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
?
-
-
-
additional information
Meyerozyma guilliermondii H17
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
centrifugation and DEAE-cellulose, partial purification
Meyerozyma guilliermondii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4
-
-
Meyerozyma guilliermondii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-amino-2-oxobutyrate + pyruvate
at 2% activity compared to L-lysine
640193
Meyerozyma guilliermondii
2,4-dioxobutanoate + L-alanine
-
-
-
?
4-amino-2-oxobutyrate + pyruvate
at 2% activity compared to L-lysine
640193
Meyerozyma guilliermondii H17
2,4-dioxobutanoate + L-alanine
-
-
-
?
L-lysine + pyruvate
-
640193
Meyerozyma guilliermondii
2-aminoadipate 6-semialdehyde + L-alanine
-
640193
Meyerozyma guilliermondii
?
L-lysine + pyruvate
involved in lysine degradation
640193
Meyerozyma guilliermondii
2-aminoadipate 6-semialdehyde + L-alanine
-
640193
Meyerozyma guilliermondii
?
L-lysine + pyruvate
-
640193
Meyerozyma guilliermondii H17
2-aminoadipate 6-semialdehyde + L-alanine
-
640193
Meyerozyma guilliermondii H17
?
L-lysine + pyruvate
involved in lysine degradation
640193
Meyerozyma guilliermondii H17
2-aminoadipate 6-semialdehyde + L-alanine
-
640193
Meyerozyma guilliermondii H17
?
additional information
D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates
640193
Meyerozyma guilliermondii
?
-
-
-
-
additional information
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
640193
Meyerozyma guilliermondii
?
-
-
-
-
additional information
D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates
640193
Meyerozyma guilliermondii H17
?
-
-
-
-
additional information
the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source
640193
Meyerozyma guilliermondii H17
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Meyerozyma guilliermondii
32
-
-
Meyerozyma guilliermondii
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
30
activity increases linearly with temperature
Meyerozyma guilliermondii
15
45
50% of maximal activity at 15°C and 45°C
Meyerozyma guilliermondii
Other publictions for EC 2.6.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722527
Chou
L-lysine catabolism is control ...
Pseudomonas aeruginosa
J. Bacteriol.
192
5874-5880
2010
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1
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1
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1
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1
1
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640194
Schmidt
A novel enzyme, L-lysine:pyruv ...
Meyerozyma guilliermondii, Meyerozyma guilliermondii H17
FEMS Microbiol. Lett.
49
203-206
1988
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1
4
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4
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1
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8
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2
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1
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1
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1
4
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1
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8
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2
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640193
Schmidt
-
Lysine degradation in Pichia g ...
Meyerozyma guilliermondii, Meyerozyma guilliermondii H17
J. Basic Microbiol.
27
595-601
1987
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12
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1
4
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4
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1
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1
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10
-
2
2
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1
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1
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12
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1
4
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1
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1
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10
-
2
2
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