BRENDA - Enzyme Database show
show all sequences of 2.6.1.67

Lysine degradation in Candida. Characterization and probable role of L-norleucine-leucine, 4-aminobutyrate and delta-aminovalerate:2-oxoglutarate aminotransferases

Der Garabedian, P.A.; Vermeersch, J.J.; Biochimie 71, 497-503 (1989)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
L-lysine
this enzyme is strongly induced in vivo by growing Candida on L-lysine
Candida guilliermondii var. membranaefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-leucine + 2-oxoglutarate
Candida guilliermondii var. membranaefaciens
-
4-methyl-2-oxopentanoate + L-glutamate
-
-
?
L-norleucine + 2-oxoglutarate
Candida guilliermondii var. membranaefaciens
-
2-oxohexanoate + L-glutamate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida guilliermondii var. membranaefaciens
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate partial purification and several chromatographic steps, all steps at 4°C
Candida guilliermondii var. membranaefaciens
Storage Stability
Storage Stability
Organism
-25°C, stable in the presence of 0.01 mM pyridoxal 5'-phosphate and 0.01 mM 2-oxoglutarate
Candida guilliermondii var. membranaefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-N-methylornithine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
5-(methylamino)-2-oxopentanoic acid + L-glutamate
-
-
-
?
6-N-acetyllysine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
6-(acetylamino)-2-oxohexanoic acid + L-glutamate
-
-
-
?
L-aspartate + 2-oxoglutarate
4.3% of activity compared to L-norleucine
640095
Candida guilliermondii var. membranaefaciens
oxaloacetate + L-glutamate
-
-
-
?
L-isoleucine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
3-methyl-2-oxopentanoate + L-glutamate
-
-
-
?
L-leucine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
4-methyl-2-oxopentanoate + L-glutamate
-
-
-
?
L-norleucine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
2-oxohexanoate + L-glutamate
-
-
-
?
L-norvaline + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
2-oxopentanoate + L-glutamate
-
-
-
?
additional information
glycine, L-alanine, L-tyrosine, beta-alanine, epsilon-aminocaproate, delta-aminovalerate, L-arginine, L-lysine or L-ornithine are no substrates
640095
Candida guilliermondii var. membranaefaciens
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Candida guilliermondii var. membranaefaciens
55
-
-
Candida guilliermondii var. membranaefaciens
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
55
reaction velocity increases linearly with increasing temperature at pH 7.8, above 55°C activity rapidly decreases, 2-oxoglutarate and to a lesser extent pyridoxal 5'-phosphate are effective protecting agents toward heating
Candida guilliermondii var. membranaefaciens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
5 min, about 20% activity remaining activity, with addition of pyridoxal 5'-phosphate 80% remaining activity, with addition of 2-oxoglutarate 100% remaining activity
Candida guilliermondii var. membranaefaciens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
L-lysine
this enzyme is strongly induced in vivo by growing Candida on L-lysine
Candida guilliermondii var. membranaefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-leucine + 2-oxoglutarate
Candida guilliermondii var. membranaefaciens
-
4-methyl-2-oxopentanoate + L-glutamate
-
-
?
L-norleucine + 2-oxoglutarate
Candida guilliermondii var. membranaefaciens
-
2-oxohexanoate + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate partial purification and several chromatographic steps, all steps at 4°C
Candida guilliermondii var. membranaefaciens
Storage Stability (protein specific)
Storage Stability
Organism
-25°C, stable in the presence of 0.01 mM pyridoxal 5'-phosphate and 0.01 mM 2-oxoglutarate
Candida guilliermondii var. membranaefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-N-methylornithine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
5-(methylamino)-2-oxopentanoic acid + L-glutamate
-
-
-
?
6-N-acetyllysine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
6-(acetylamino)-2-oxohexanoic acid + L-glutamate
-
-
-
?
L-aspartate + 2-oxoglutarate
4.3% of activity compared to L-norleucine
640095
Candida guilliermondii var. membranaefaciens
oxaloacetate + L-glutamate
-
-
-
?
L-isoleucine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
3-methyl-2-oxopentanoate + L-glutamate
-
-
-
?
L-leucine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
4-methyl-2-oxopentanoate + L-glutamate
-
-
-
?
L-norleucine + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
2-oxohexanoate + L-glutamate
-
-
-
?
L-norvaline + 2-oxoglutarate
-
640095
Candida guilliermondii var. membranaefaciens
2-oxopentanoate + L-glutamate
-
-
-
?
additional information
glycine, L-alanine, L-tyrosine, beta-alanine, epsilon-aminocaproate, delta-aminovalerate, L-arginine, L-lysine or L-ornithine are no substrates
640095
Candida guilliermondii var. membranaefaciens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Candida guilliermondii var. membranaefaciens
55
-
-
Candida guilliermondii var. membranaefaciens
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
55
reaction velocity increases linearly with increasing temperature at pH 7.8, above 55°C activity rapidly decreases, 2-oxoglutarate and to a lesser extent pyridoxal 5'-phosphate are effective protecting agents toward heating
Candida guilliermondii var. membranaefaciens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
5 min, about 20% activity remaining activity, with addition of pyridoxal 5'-phosphate 80% remaining activity, with addition of 2-oxoglutarate 100% remaining activity
Candida guilliermondii var. membranaefaciens
Other publictions for EC 2.6.1.67
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
640095
Der Garabedian
Lysine degradation in Candida. ...
Candida guilliermondii var. membranaefaciens
Biochimie
71
497-503
1989
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
1
8
-
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
8
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
640188
Der Garabedian
Candida L-norleucine,leucine:2 ...
Candida guilliermondii var. membranaefaciens
Eur. J. Biochem.
167
141-147
1987
-
-
-
-
-
-
4
3
-
-
2
2
-
3
-
-
1
-
-
-
1
1
8
1
2
1
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
3
-
-
2
2
-
-
-
1
-
-
1
1
8
1
2
1
1
-
1
-
-
-
-
-
-
-
-
-