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Literature summary for 2.6.1.64 extracted from
- Purification of soluble and mitochondrial glutamine transaminase K from rat kidney. Use of a sensitive assay involving transamination between L-phenylalanine and alpha-keto-gamma-methiolbutyrate (1978), Anal. Biochem., 89, 451-460.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, SDS-PAGE | Rattus norvegicus |
| 100000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + phenylpyruvate | Rattus norvegicus | - |
2-oxoglutaramate + L-phenylalanine | - |
r |  |
| L-glutamine + phenylpyruvate | Rattus norvegicus Wistar | - |
2-oxoglutaramate + L-phenylalanine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar | - |
| Rattus norvegicus Wistar | - |
Wistar | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | K-form | Rattus norvegicus | - |
| liver | L-form | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.65 | - |
mitochondrial isozyme | Rattus norvegicus |
| 6.48 | - |
cytoplasmic isozyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, both isozymes stored in 200 mM potassium phosphate buffer, pH 7.2, 20% glycerol and 5 mM 2-mercaptoethanol, stable for at least 2 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + phenylpyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-phenylalanine | - |
r | |
| L-glutamine + phenylpyruvate | - |
Rattus norvegicus Wistar | 2-oxoglutaramate + L-phenylalanine | - |
r | |
| L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate | - |
Rattus norvegicus | phenylpyruvate + L-methionine | - |
r | |
| L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate | - |
Rattus norvegicus Wistar | phenylpyruvate + L-methionine | - |
r | |
| additional information | identical with histidine-pyruvate transaminase II and phenylalanine-alpha-keto-gamma-methiolbutyrate transaminase | Rattus norvegicus | ? | - |
? | |
| additional information | identical with histidine-pyruvate transaminase II and phenylalanine-alpha-keto-gamma-methiolbutyrate transaminase | Rattus norvegicus Wistar | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutamine transaminase K | - |
Rattus norvegicus |
| glutamine transaminase L | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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