KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-Oxoglutaramate | pH 8.5, 37°C, L-phenylalanine as amino group donor | Rattus norvegicus | |
0.58 | - |
L-phenylalanine | pH 8.5, 37°C | Rattus norvegicus | |
0.92 | - |
alpha-keto-gamma-methiolbutyrate | pH 8.5, 37°C, L-phenylalanine as amino group donor | Rattus norvegicus | |
1.4 | - |
L-glutamine | pH 8.5, 37°C, phenylpyruvate | Rattus norvegicus | |
4.2 | - |
L-methionine | pH 8.5, 37°C, phenylpyruvate | Rattus norvegicus | |
4.4 | - |
L-ethionine | pH 8.5, 37°C, phenylpyruvate | Rattus norvegicus | |
4.8 | - |
glyoxylate | pH 8.5, 37°C | Rattus norvegicus | |
7.8 | - |
alpha-keto-n-valerate | pH 8.5, 37°C, L-phenylalanine as amino group donor | Rattus norvegicus | |
8.7 | - |
alpha-keto-n-caproate | pH 8.5, 37°C, L-phenylalanine as amino group donor | Rattus norvegicus | |
18 | - |
alpha-Ketobutyrate | pH 8.5, 37°C | Rattus norvegicus | |
27 | - |
pyruvate | pH 8.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, liver enzyme | Rattus norvegicus |
110000 | - |
kidney enzyme, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
cardiac muscle | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
additional information | no glutamine transaminase activity in skeletal muscle, testis, spleen, pancreas, lung and intestine | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.68 | - |
cytoplasmic isozyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-cyano-L-alpha-aminobutyrate + glyoxylate | - |
Rattus norvegicus | 4-cyano-2-oxobutanoate + glycine | - |
r | |
4-cyano-L-alpha-aminobutyrate + phenylpyruvate | - |
Rattus norvegicus | 4-cyano-2-oxobutanoate + L-phenylalanine | - |
r | |
albizziin + glyoxylate | - |
Rattus norvegicus | 3-carbamido-2-oxopropanoate + glycine | - |
r | |
DL-homoserine + glyoxylate | - |
Rattus norvegicus | 4-hydroxy-2-oxobutanoate + glycine | - |
r | |
DL-homoserine + phenylpyruvate | - |
Rattus norvegicus | 4-hydroxy-2-oxobutanoate + phenylalanine | - |
r | |
L-cysteine + glyoxylate | - |
Rattus norvegicus | 3-mercapto-2-oxopropanoate + glycine | - |
r | |
L-cysteine + phenylpyruvate | - |
Rattus norvegicus | 3-mercapto-2-oxopropanoate + L-phenylalanine | - |
r | |
L-ethionine + glyoxylate | - |
Rattus norvegicus | 4-ethylsulfanyl-2-oxobutanoate + glycine | - |
r | |
L-ethionine + phenylpyruvate | - |
Rattus norvegicus | 4-ethylsulfanyl-2-oxobutanoate + L-phenylalanine | - |
r | |
L-gamma-glutamylhydrazone of glyoxylate + glyoxylate | - |
Rattus norvegicus | ? | - |
r | |
L-gamma-glutamylhydrazone of glyoxylate + phenylpyruvate | - |
Rattus norvegicus | ? | - |
r | |
L-gamma-glutamylhydrazone of phenylpyruvate + glyoxylate | - |
Rattus norvegicus | ? | - |
r | |
L-gamma-glutamylhydrazone of phenylpyruvate + phenylpyruvate | - |
Rattus norvegicus | ? | - |
r | |
L-glutamic acid-gamma-methyl ester + glyoxylate | liver enzyme L | Rattus norvegicus | 2-oxo-pentandioate-5-methyl ester + glycine | - |
r | |
L-glutamic acid-gamma-methyl ester + phenylpyruvate | liver enzyme L | Rattus norvegicus | 2-oxo-pentandioate-5-methyl ester + L-phenylalanine | - |
r | |
L-glutamine + 2-oxobutyrate | - |
Rattus norvegicus | 2-oxoglutaramate + 2-aminobutanoate | - |
r | |
L-glutamine + 2-oxoglutaramate | - |
Rattus norvegicus | 2-oxoglutaramate + L-glutamine | - |
r | |
L-glutamine + 2-oxoglutarate | - |
Rattus norvegicus | 2-oxoglutaramate + L-glutamate | - |
r | |
L-glutamine + 2-oxovalerate | - |
Rattus norvegicus | 2-oxoglutaramate + L-norvaline | - |
r | |
L-glutamine + 3-hydroxypyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-serine | - |
r | |
L-glutamine + alpha-keto-delta-carbamidovalerate | - |
Rattus norvegicus | 2-oxoglutaramate + L-arginine | - |
r | |
L-glutamine + alpha-keto-n-caproate | - |
Rattus norvegicus | 2-oxoglutaramate + L-norleucine | - |
r | |
L-glutamine + alpha-ketoisocaproate | - |
Rattus norvegicus | 2-oxoglutaramate + L-leucine | - |
r | |
L-glutamine + alpha-ketoisovalerate | - |
Rattus norvegicus | 2-oxoglutaramate + L-valine | - |
r | |
L-glutamine + glyoxylate | isozyme L highly active, isozyme K relatively little activity toward glyoxylate | Rattus norvegicus | 2-oxoglutaramate + glycine | - |
r | |
L-glutamine + oxaloacetate | - |
Rattus norvegicus | 2-oxoglutaramate + L-aspartate | - |
r | |
L-glutamine + p-hydroxyphenylpyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-tyrosine | - |
r | |
L-glutamine + phenylpyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-phenylalanine | - |
r | |
L-glutamine + pyruvate | isozyme L highly active, isozyme K relatively little activity toward pyruvate | Rattus norvegicus | 2-oxoglutaramate + L-alanine | - |
r | |
L-glutamylmethylamide + glyoxylate | liver enzyme L | Rattus norvegicus | 2-oxoglutarate-5-methylamide + glycine | - |
r | |
L-glutamylmethylamide + phenylpyruvate | - |
Rattus norvegicus | 2-oxoglutarate-5-methylamide + L-phenylalanine | - |
r | |
L-histidine + pyruvate | poor substrates | Rattus norvegicus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine | - |
r | |
L-methionine + 2-oxoglutaramate | isozymes L and K highly active | Rattus norvegicus | L-glutamine + 4-methylsulfanyl-2-oxobutanoate | - |
r | |
L-methionine + glyoxylate | - |
Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate + glycine | - |
r | |
L-methionine + phenylpyruvate | - |
Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine | - |
r | |
L-methionine sulfone + glyoxylate | - |
Rattus norvegicus | 4-methylsulfonyl-2-oxobutanoate + glycine | - |
r | |
L-methionine sulfone + phenylpyruvate | - |
Rattus norvegicus | 4-methylsulfonyl-2-oxobutanoate + L-phenylalanine | - |
r | |
L-methionine-SR-sulfoxide + glyoxylate | - |
Rattus norvegicus | 4-methylsulfinyl-2-oxobutanoate + glycine | - |
r | |
L-methionine-SR-sulfoxide + phenylpyruvate | - |
Rattus norvegicus | 4-methylsulfinyl-2-oxobutanoate + L-phenylalanine | - |
r | |
L-methionine-SR-sulfoximine + glyoxylate | - |
Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + glycine | - |
r | |
L-methionine-SR-sulfoximine + phenylpyruvate | - |
Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + L-phenylalanine | - |
r | |
L-phenylalanine + 2-oxoglutaramate | - |
Rattus norvegicus | phenylpyruvate + L-glutamine | - |
r | |
L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate | - |
Rattus norvegicus | phenylpyruvate + L-methionine | - |
r | |
L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate | isozyme K very active toward phenylalanine | Rattus norvegicus | phenylpyruvate + L-methionine | - |
r | |
L-phenylalanine + alpha-keto-n-caproate | - |
Rattus norvegicus | phenylpyruvate + L-norleucine | - |
r | |
L-phenylalanine + alpha-keto-n-valerate | - |
Rattus norvegicus | phenylpyruvate + L-norvaline | - |
r | |
L-phenylalanine + alpha-ketobutyrate | - |
Rattus norvegicus | phenylpyruvate + 2-aminobutyrate | - |
r | |
L-phenylalanine + glyoxylate | - |
Rattus norvegicus | phenylpyruvate + glycine | - |
r | |
L-phenylalanine + pyruvate | - |
Rattus norvegicus | phenylpyruvate + L-alanine | - |
r | |
L-tyrosine + 2-oxoglutaramate | - |
Rattus norvegicus | 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamine | - |
r | |
additional information | 4 separate isozymes, cytoplasmic and mitochondrial, in kidney and liver, trimethylpyruvate and D and L-alpha-keto-beta-methylvalerate are no amino acceptors, L-gamma-glutamyldimethylamide is no substrate | Rattus norvegicus | ? | - |
? | |
O-carbamoyl-L-serine + glyoxylate | - |
Rattus norvegicus | O-carbamoyl-(3-hydroxy-2-oxo-propanoate) + glycine | - |
r | |
S-carbamoyl-L-cysteine + glyoxylate | - |
Rattus norvegicus | S-carbamoyl-3-mercapto-2-oxo-propanoate + glycine | - |
r | |
S-methyl-L-cysteine + glyoxylate | - |
Rattus norvegicus | 3-methylsulfanyl-2-oxopropanoate + glycine | - |
r | |
S-methyl-L-cysteine + phenylpyruvate | - |
Rattus norvegicus | 3-methylsulfanyl-2-oxopropanoate + L-phenylalanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, liver enzyme | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
glutamine transaminase K | - |
Rattus norvegicus |
glutamine transaminase L | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
liver glutaminase transaminase L is unstable at 60°C in absence of an added alpha-keto-acid, kidney glutamine transaminase L is stable | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rattus norvegicus |