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Literature summary for 2.6.1.62 extracted from

  • Breen, R.S.; Campopiano, D.J.; Webster, S.; Brunton, M.; Watt, R.; Baxter, R.L.
    The mechanism of 7,8-diaminopelargonate synthase; the role of S-adenosylmethionine as the amino donor (2003), Org. Biomol. Chem., 1, 3498-3499.
    View publication on PubMed
Search for more literature for 2.6.1.62

Cloned(Commentary)

Cloned (Comment) Organism
cloning from strain JM101, overexpression in strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
amiclenomycin
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate Escherichia coli enzyme is obligate catalyzing the second step in the biosynthesis of vitamin H, i.e. biotin S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from strain BL21(DE3) Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate ping pong kinetics, reaction mechanism, via a enzyme-pyridoxamine phosphate, a quinonoid intermediate Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate enzyme is obligate catalyzing the second step in the biosynthesis of vitamin H, i.e. biotin Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 r
S-adenosyl-L-methionine + 8-amino-7-oxononanoate stereospecific amination Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 r

Synonyms

Synonyms Comment Organism
7,8-diaminopelargonate synthase
-
 Escherichia coli
DAPA synthase
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Escherichia coli