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Literature summary for 2.6.1.60 extracted from

  • Harada, I.; Noguchi, T.; Kido, R.
    Purification and characterization of aromatic-amino-acid-glyoxylate aminotransferase from monkey and rat liver (1978), Hoppe-Seyler's Z. Physiol. Chem., 359, 481-488.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
Macaca fuscata
hydroxylamine
-
Rattus norvegicus
Isonicotinic hydrazide
-
Macaca fuscata
Isonicotinic hydrazide
-
Rattus norvegicus
KCN
-
Macaca fuscata
KCN
-
Rattus norvegicus
Semicarbazide strong Macaca fuscata
Semicarbazide strong Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
glyoxylate cosubstrate L-kynurenine, pH 8.0, 37°C Macaca fuscata
0.5
-
glyoxylate L-kynurenine, pH 8.0, 37°C Rattus norvegicus
1.2
-
L-kynurenine glyoxylate, pH 8.0, 37°C Rattus norvegicus
1.5
-
L-kynurenine cosubstrate glyoxylate, pH 8.0, 37°C Macaca fuscata

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-
mitochondrion
-
Macaca fuscata 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Rattus norvegicus
40000
-
2 * 40000, SDS-PAGE Macaca fuscata
80000
-
gel filtration Rattus norvegicus
80000
-
gel filtration Macaca fuscata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an aromatic amino acid + glyoxylate Rattus norvegicus
-
an aromatic oxo acid + glycine
-
?

Organism

Organism UniProt Comment Textmining
Macaca fuscata
-
Blyth monkey
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
partial Macaca fuscata

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Macaca fuscata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.105
-
pH 7.5, 37°C Macaca fuscata
0.223
-
pH 7.5, 37°C Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, at least 4 weeks Macaca fuscata
-20°C, at least 7 weeks Rattus norvegicus
0-4°C, 2 weeks with little or no loss of activity Macaca fuscata
0-4°C, 3 weeks with little or no loss of activity respectively Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxytryptophan + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
?
5-hydroxytryptophan + glyoxylate less efficient amino group donor than L-phenylalanine Macaca fuscata 3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
?
an aromatic amino acid + glyoxylate
-
Rattus norvegicus an aromatic oxo acid + glycine
-
?
L-histidine + glyoxylate
-
Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
?
L-histidine + glyoxylate
-
Macaca fuscata 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
?
L-kynurenine + glyoxylate
-
Macaca fuscata 3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
?
L-kynurenine + glyoxylate poor amino group donor Rattus norvegicus 3-(2-aminophenyl-)2,4-dioxobutanoate + glycine
-
?
L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
Rattus norvegicus phenylpyruvate + L-methionine
-
?
L-phenylalanine + 2-oxo-4-methylthiobutyrate
-
Macaca fuscata phenylpyruvate + L-methionine
-
?
L-phenylalanine + 2-oxoglutarate
-
Rattus norvegicus phenylpyruvate + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate
-
Macaca fuscata phenylpyruvate + L-glutamate
-
?
L-phenylalanine + glyoxylate
-
Rattus norvegicus phenylpyruvate + glycine
-
?
L-phenylalanine + glyoxylate
-
Macaca fuscata phenylpyruvate + glycine
-
?
L-phenylalanine + hydroxypyruvate
-
Rattus norvegicus phenylpyruvate + L-serine
-
?
L-phenylalanine + hydroxypyruvate
-
Macaca fuscata phenylpyruvate + L-serine
-
?
L-phenylalanine + pyruvate
-
Rattus norvegicus phenylpyruvate + L-alanine
-
?
L-phenylalanine + pyruvate
-
Macaca fuscata phenylpyruvate + L-alanine
-
?
tryptophan + glyoxylate less efficient amino group donor than L-phenylalanine Rattus norvegicus 3-indole-2-oxopropanoate + glycine
-
?
tryptophan + glyoxylate less efficient amino group donor than L-phenylalanine Macaca fuscata 3-indole-2-oxopropanoate + glycine
-
?
tyrosine + glyoxylate
-
Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
?
tyrosine + glyoxylate
-
Macaca fuscata 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Rattus norvegicus
dimer 2 * 40000, SDS-PAGE Macaca fuscata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus
37
-
assay at Macaca fuscata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 8 Rattus norvegicus
additional information
-
pI: 8 Macaca fuscata
8 8.5
-
Rattus norvegicus
8 8.5
-
Macaca fuscata

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement Rattus norvegicus
pyridoxal 5'-phosphate requirement Macaca fuscata