Cloned (Comment) | Organism |
---|---|
gene aro8, recombinant overexpression in Escherichia coli strain Rosetta (DE3) pLysS, subcloning in Escherichia coli strain TOP10F' | Candida albicans |
gene aro9, recombinant overexpression in Escherichia coli strain Rosetta (DE3) pLysS, subcloning in Escherichia coli strain TOP10F' | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a DELTAaro9 knockout strain, also of a double mutant with aro8, overview | Candida albicans |
additional information | construction of a DELTAyer152C knockout strain, also of a double mutant with aro8, overview | Candida albicans |
additional information | construction of several DELTAaro8 knockout strains, also double mutants with aro9 and yer152C, genotypes, overview | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(aminooxy)acetate | AOA, is a broad-spectrum inhibitor of pyridoxal phosphate-dependent enzymatic reactions, such as transaminations. AOA strongly inhibits the growth of Candida albicans in minimal medium with selected amino acids as the sole nitrogen source in an amino acid concentration-dependent manner; AOA, is a broad-spectrum inhibitor of pyridoxal phosphate-dependent enzymatic reactions, such as transaminations. AOA strongly inhibits the growth of Candida albicans in minimal medium with selected amino acids as the sole nitrogen source in an amino acid concentration-dependent manner; AOA, is a broad-spectrum inhibitor of pyridoxal phosphate-dependent enzymatic reactions, such as transaminations. AOA strongly inhibits the growth of Candida albicans in minimal medium with selected amino acids as the sole nitrogen source in an amino acid concentration-dependent manner | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
alpha-aminoadipate | pH and temperature not specified in the publication, with 2-oxoglutarate | Candida albicans | |
0.017 | - |
phenylpyruvate | pH and temperature not specified in the publication, with L-glutamate | Candida albicans | |
0.026 | - |
4-hydroxyphenylpyruvate | pH and temperature not specified in the publication, with L-glutamate | Candida albicans | |
0.053 | - |
L-phenylalanine | pH and temperature not specified in the publication, with 2-oxoglutarate | Candida albicans | |
0.09 | - |
L-tyrosine | pH and temperature not specified in the publication, with 2-oxoglutarate | Candida albicans | |
0.1 | - |
2-oxoadipate | pH and temperature not specified in the publication, with L-glutamate | Candida albicans | |
0.13 | - |
2-oxoadipate | pH and temperature not specified in the publication, with L-glutamate | Candida albicans | |
0.24 | - |
L-phenylalanine | pH and temperature not specified in the publication | Candida albicans | |
0.27 | - |
2-oxoglutarate | pH and temperature not specified in the publication | Candida albicans | |
0.41 | - |
2-oxoglutarate | pH and temperature not specified in the publication | Candida albicans | |
0.46 | - |
L-tyrosine | pH and temperature not specified in the publication | Candida albicans | |
1.07 | - |
L-tryptophan | pH and temperature not specified in the publication | Candida albicans | |
1.68 | - |
L-tryptophan | pH and temperature not specified in the publication, with 2-oxoglutarate | Candida albicans | |
1.8 | - |
L-tryptophan | pH and temperature not specified in the publication | Candida albicans | |
2.8 | - |
L-tyrosine | pH and temperature not specified in the publication | Candida albicans | |
4.28 | - |
alpha-aminoadipate | pH and temperature not specified in the publication | Candida albicans | |
4.76 | - |
L-phenylalanine | pH and temperature not specified in the publication | Candida albicans | |
11.5 | - |
L-histidine | pH and temperature not specified in the publication, with 2-oxoglutarate | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-phenylalanine | Candida albicans | - |
L-glutamate + phenylpyruvate | - |
r | |
2-oxoglutarate + L-phenylalanine | Candida albicans ATCC MYA-2876 | - |
L-glutamate + phenylpyruvate | - |
r | |
2-oxoglutarate + L-tryptophan | Candida albicans | - |
L-glutamate + 3-indole-2-oxopropanoate | - |
r | |
2-oxoglutarate + L-tryptophan | Candida albicans ATCC MYA-2876 | - |
L-glutamate + 3-indole-2-oxopropanoate | - |
r | |
2-oxoglutarate + L-tyrosine | Candida albicans | - |
L-glutamate + 4-hydroxyphenylpyruvate | - |
r | |
2-oxoglutarate + L-tyrosine | Candida albicans ATCC MYA-2876 | - |
L-glutamate + 4-hydroxyphenylpyruvate | - |
r | |
alpha-aminoadipate + 2-oxoglutarate | Candida albicans | - |
2-oxoadipate + L-glutamate | - |
r | |
alpha-aminoadipate + 2-oxoglutarate | Candida albicans ATCC MYA-2876 | - |
2-oxoadipate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | A0A1D8PG20 | - |
- |
Candida albicans | A0A1D8PMC5 | - |
- |
Candida albicans | A0A1D8PPA8 | - |
- |
Candida albicans ATCC MYA-2876 | A0A1D8PG20 | - |
- |
Candida albicans ATCC MYA-2876 | A0A1D8PMC5 | - |
- |
Candida albicans ATCC MYA-2876 | A0A1D8PPA8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoadipate + L-glutamate | - |
Candida albicans | alpha-aminoadipate + 2-oxoglutarate | - |
r | |
2-oxoglutarate + L-histidine | - |
Candida albicans | L-glutamate + 3-(1H-imidazol-4-yl)-2-oxopropanoate | - |
r | |
2-oxoglutarate + L-phenylalanine | - |
Candida albicans | L-glutamate + phenylpyruvate | - |
r | |
2-oxoglutarate + L-phenylalanine | - |
Candida albicans ATCC MYA-2876 | L-glutamate + phenylpyruvate | - |
r | |
2-oxoglutarate + L-tryptophan | - |
Candida albicans | L-glutamate + 3-indole-2-oxopropanoate | - |
r | |
2-oxoglutarate + L-tryptophan | - |
Candida albicans ATCC MYA-2876 | L-glutamate + 3-indole-2-oxopropanoate | - |
r | |
2-oxoglutarate + L-tyrosine | - |
Candida albicans | L-glutamate + 4-hydroxyphenylpyruvate | - |
r | |
2-oxoglutarate + L-tyrosine | - |
Candida albicans ATCC MYA-2876 | L-glutamate + 4-hydroxyphenylpyruvate | - |
r | |
alpha-aminoadipate + 2-oxoglutarate | - |
Candida albicans | 2-oxoadipate + L-glutamate | - |
r | |
alpha-aminoadipate + 2-oxoglutarate | - |
Candida albicans ATCC MYA-2876 | 2-oxoadipate + L-glutamate | - |
r | |
L-glutamate + 4-hydroxyphenylpyruvate | - |
Candida albicans | 2-oxoglutarate + L-tyrosine | - |
r | |
L-glutamate + 4-hydroxyphenylpyruvate | - |
Candida albicans ATCC MYA-2876 | 2-oxoglutarate + L-tyrosine | - |
r | |
L-glutamate + phenylpyruvate | - |
Candida albicans | 2-oxoglutarate + L-phenylalanine | - |
r | |
L-glutamate + phenylpyruvate | - |
Candida albicans ATCC MYA-2876 | 2-oxoglutarate + L-phenylalanine | - |
r | |
additional information | no activity with L-histidine | Candida albicans | ? | - |
- |
|
additional information | no activity with L-histidine | Candida albicans ATCC MYA-2876 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Aro8 | - |
Candida albicans |
ARO9 | - |
Candida albicans |
aromatic-amino-acid:2-oxoglutarate transaminase | UniProt | Candida albicans |
bifunctional 2-aminoadipate transaminase/aromatic-amino-acid:2-oxoglutarate transaminase | UniProt | Candida albicans |
CaAro8p | - |
Candida albicans |
CaAro9p | - |
Candida albicans |
CaYer152Cp | - |
Candida albicans |
Yer152C | - |
Candida albicans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP | Candida albicans |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of transaminase enzymes by (aminooxy)acetate (AOA), a broad-spectrum inhibitor of pyridoxal phosphate-dependent enzymatic reactions, strongly inhibits the growth of Candida albicans in minimal medium with selected amino acids as the sole nitrogen source in an amino acid concentration-dependent manner | Candida albicans |
metabolism | enzyme CaAro8p is involved in the catabolism of histidine, lysine, and aromatic amino acids as well as in L-Lys, L-Phe, and L-Tyr biosynthesis | Candida albicans |
metabolism | enzyme Cayer152Cp is active with aromatic amino acids but does not play an important role in vivo in aromatic acid catabolism and biosynthesis | Candida albicans |
metabolism | the enzyme CaAro9p participates in the catabolism of aromatic amino acids and lysine at high concentrations of these compounds, with no biosynthetic role | Candida albicans |