KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
2-oxoglutarate | pH 7.6, 80°C | Pyrococcus furiosus | |
1.15 | - |
L-phenylalanine | pH 7.6, 80°C | Pyrococcus furiosus | |
1.31 | - |
L-tyrosine | pH 7.6, 80°C | Pyrococcus furiosus | |
2.1 | - |
L-tryptophan | pH 7.6, 80°C | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, SDS-PAGE | Pyrococcus furiosus |
92000 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
exposure to 02 does not affect enzyme activity | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 2-oxoglutarate | - |
Pyrococcus furiosus | phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Pyrococcus furiosus | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Pyrococcus furiosus | p-hydroxyphenylpyruvate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 44000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
ArAT | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
pyridoxal 5'-phosphate and 2-ketoglutarate stabilize the enzyme at extreme temperatures | Pyrococcus furiosus |
80 | - |
irreversible denaturation of the pure enzyme is not apparent after 6 h in the presence of pyridoxal 5'-phosphate and 2-ketoglutarate. The apoenzyme (lacking pyridoxal 5'-phosphate) loses 70% of its activity (measured after reconstitution) after 6 h | Pyrococcus furiosus |
95 | - |
half-life: 16 h in the presence of pyridoxal 5'-phosphate and 2-ketoglutarate, 12 h if cofactor and substrate are not added | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
62 | - |
L-tryptophan | pH 7.6, 80°C | Pyrococcus furiosus | |
72 | - |
L-tyrosine | pH 7.6, 80°C | Pyrococcus furiosus | |
225 | - |
2-oxoglutarate | pH 7.6, 80°C | Pyrococcus furiosus | |
253 | - |
L-phenylalanine | pH 7.6, 80°C | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pyrococcus furiosus |