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Literature summary for 2.6.1.57 extracted from

  • Cho, B.; Seo, J.; Kim, J.; Lee, C.; Kim, B.
    Asymmetric synthesis of unnatural L-amino acids using thermophilic aromatic L-amino acid transaminase (2006), Biotechnol. Bioprocess Eng., 11, 299-305.
No PubMed abstract available
Search for more literature for 2.6.1.57

Application

Application Comment Organism
synthesis asymmetric synthesis of L-homophenylalanine and L-phenylglycine from 2-oxo-4-phenylbutyrate and phenylglyoxylate, respectively, using L-glutamate as an amino donor. AroATBs can be used as a biocatalyst for the synthesis of unnatural L-amino acids by taking advantage of the large solubility difference between substrate and product at higher temperatures to accelerate an equilibrium shift favoring product formation Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) T30
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + 2-oxo-4-phenylbutyrate
-
 Bacillus sp. (in: Bacteria) L-homophenylalanine + 2-oxoglutarate
-
 r
L-glutamate + 2-oxo-4-phenylbutyrate
-
 Bacillus sp. (in: Bacteria) T30 L-homophenylalanine + 2-oxoglutarate
-
 r
L-glutamate + phenylglyoxylate
-
 Bacillus sp. (in: Bacteria) L-phenylglycine + 2-oxoglutarate
-
 r
L-glutamate + phenylglyoxylate
-
 Bacillus sp. (in: Bacteria) T30 L-phenylglycine + 2-oxoglutarate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 80 37°C: about 35% of maximal activity, 50°C: about 90% of maximal activity, 80°C: about 50% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 half-life: 28 h Bacillus sp. (in: Bacteria)
60
-
 half-life: 12 h Bacillus sp. (in: Bacteria)
70
-
 half-life: 4.2 h Bacillus sp. (in: Bacteria)
80
-
 half-life: 1.1 h Bacillus sp. (in: Bacteria)