Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
phenylpyruvate | cosubstrate glutamate | Klebsiella aerogenes | |
0.08 | - |
L-phenylalanine | cosubstrate 2-oxoglutarate | Klebsiella aerogenes | |
0.12 | - |
oxaloacetate | cosubstrate tryptophan | Klebsiella aerogenes | |
0.6 | - |
L-tryptophan | cosubstrate 2-oxoglutarate | Klebsiella aerogenes | |
2.08 | - |
2-oxoglutarate | cosubstrate tryptophan | Klebsiella aerogenes | |
70 | - |
L-histidine | cosubstrate 2-oxoglutarate | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Klebsiella aerogenes |
90000 | - |
gel filtration | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
aromatic aminotransferase I | - |
Purification (Comment) | Organism |
---|---|
protamine, ammonium sulfate, DEAE-Sephadex, ultrogel AcA44, hydroxylapatite, isoelectric focusing | Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
4°C, glycerol, 10% stabilizes during storage | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidine + 2-oxoglutarate | - |
Klebsiella aerogenes | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Klebsiella aerogenes | phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Klebsiella aerogenes | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tryptophan + oxaloacetate | - |
Klebsiella aerogenes | 3-indole-2-oxopropanoate + L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Klebsiella aerogenes |
Synonyms | Comment | Organism |
---|---|---|
aromatic amino transferase I | - |
Klebsiella aerogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, 35% loss of activity | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with tryptophan | Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.6 | approx. 50% of maximal activity at pH 6.0 and pH 9.6 | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Klebsiella aerogenes |