KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-tyrosine | pH 8.0 | Brevibacillus brevis | |
0.15 | - |
2-oxoglutarate | pH 8.0 | Brevibacillus brevis | |
0.17 | - |
L-phenylalanine | pH 8.0 | Brevibacillus brevis | |
0.21 | - |
L-tryptophan | pH 8.0 | Brevibacillus brevis | |
3.7 | - |
L-aspartate | pH 8.0 | Brevibacillus brevis | |
5.1 | - |
oxaloacetate | pH 8.0 | Brevibacillus brevis | |
7.1 | - |
L-leucine | pH 8.0 | Brevibacillus brevis | |
8.8 | - |
pyruvate | pH 8.0 | Brevibacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Brevibacillus brevis |
71000 | - |
gel filtration | Brevibacillus brevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus brevis | - |
gramicidin S-producing | - |
Purification (Comment) | Organism |
---|---|
gramicidin S-producing strain, ammonium sulfate, heat treatment, DEAE-Sephadex, hydroxylapatite, pyridoxamine 5'-phosphate-Sepharose, Ultrogel Aca-34 | Brevibacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
353 | - |
- |
Brevibacillus brevis |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, 10 mM DTT, 0.1 mM pyridoxal phosphate, at least 3 months, no loss of activity | Brevibacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aspartate + 2-oxoglutarate | 3.1% of the activity with phenylalanine | Brevibacillus brevis | oxaloacetate + L-glutamate | - |
? | |
L-histidine + 2-oxoglutarate | 27.8% of activity with phenylalanine | Brevibacillus brevis | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Brevibacillus brevis | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-methionine + 2-oxoglutarate | 0.6% of activity with phenylalanine | Brevibacillus brevis | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Brevibacillus brevis | phenylpyruvate + L-glutamate | - |
? | |
L-phenylalanine + oxaloacetate | 3.5% of activity with 2-oxoglutarate | Brevibacillus brevis | phenylpyruvate + L-aspartate | - |
? | |
L-phenylalanine + pyruvate | 2.1% of the activity with 2-oxoglutarate | Brevibacillus brevis | phenylpyruvate + L-alanine | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Brevibacillus brevis | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Brevibacillus brevis | p-hydroxyphenylpyruvate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Brevibacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Brevibacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | one mol of pyridoxal phosphate per subunit | Brevibacillus brevis |