General Stability | Organism |
---|---|
stable to freezing and thawing in phosphate buffers supplemented with pyridoxal phosphate, 2-oxoglutarate, dithiothreitol and EDTA | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
L-phenylalanine | pH 7.3, 37°C | Escherichia coli | |
0.62 | - |
L-tyrosine | pH 7.3, 37°C | Escherichia coli | |
2.5 | - |
2-oxoglutarate | pH 7.3, 37°C | Escherichia coli | |
3.13 | - |
oxaloacetate | pH 7.3, 37°C | Escherichia coli | |
10 | - |
L-tryptophan | pH 7.3, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, 55°C, 5 min, calcium phosphate gel, DEAE-cellulose, isoelectric focusing | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70.2 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-25°C, protein concentration: 0.03-0.2 mg/ml, potassium phosphate pH 7.0, 1 mM EDTA, 1 mM dithiothreitol, 2 mM 2-oxoglutarate and 0.2 mM pyridoxal 5'-phosphate, several months, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-iodo-L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | 3-(3-iodophenyl)-2-oxopropanoate + L-glutamate | - |
? | |
3-iodo-L-tyrosine + oxaloacetate | - |
Escherichia coli | 3-(3-iodophenyl)-2-oxopropanoate + L-aspartate | - |
? | |
aspartate + 2-oxoglutarate | weak activity | Escherichia coli | oxaloacetate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | weak activity | Escherichia coli | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-methionine + 2-oxoglutarate | weak activity | Escherichia coli | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | phenylpyruvate + L-glutamate | - |
? | |
L-phenylalanine + oxaloacetate | - |
Escherichia coli | phenylpyruvate + L-aspartate | - |
? | |
L-tryptophan + 2-oxoglutarate | weak activity | Escherichia coli | 3-indole-2-oxopropanoate + L-glutamate | - |
r | |
L-tryptophan + oxaloacetate | - |
Escherichia coli | 3-indole-2-oxopropanoate + L-aspartate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | p-hydroxyphenylpyruvate + L-glutamate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5% loss of activity after 5 min in the presence of 0.2 mM pyridoxal 5'-phosphate and 2 mM 2-oxoglutarate, 60% loss of activity in the absence of cofactor and substrate after 5 min, more than 90% after 10 min | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
152 | - |
L-phenylalanine | pH 7.3, 37°C | Escherichia coli | |
250 | - |
L-tryptophan | pH 7.3, 37°C | Escherichia coli | |
278 | - |
2-oxoglutarate | pH 7.3, 37°C | Escherichia coli | |
333 | - |
L-tyrosine | pH 7.3, 37°C | Escherichia coli | |
500 | - |
oxaloacetate | pH 7.3, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
tryptophan | Escherichia coli |
8 | - |
with tyrosine | Escherichia coli |
8 | - |
with phenylalanine | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Escherichia coli |