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Literature summary for 2.6.1.57 extracted from

  • Mavrides, C.
    Transamination of aromatic amino acids in Escherichia coli (1987), Methods Enzymol., 142, 253-267.
    View publication on PubMed

General Stability

General Stability Organism
stable to freezing and thawing in phosphate buffers supplemented with pyridoxal phosphate, 2-oxoglutarate, dithiothreitol and EDTA Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
L-phenylalanine pH 7.3, 37°C Escherichia coli
0.62
-
L-tyrosine pH 7.3, 37°C Escherichia coli
2.5
-
2-oxoglutarate pH 7.3, 37°C Escherichia coli
3.13
-
oxaloacetate pH 7.3, 37°C Escherichia coli
10
-
L-tryptophan pH 7.3, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, 55°C, 5 min, calcium phosphate gel, DEAE-cellulose, isoelectric focusing Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70.2
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-25°C, protein concentration: 0.03-0.2 mg/ml, potassium phosphate pH 7.0, 1 mM EDTA, 1 mM dithiothreitol, 2 mM 2-oxoglutarate and 0.2 mM pyridoxal 5'-phosphate, several months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-iodo-L-tyrosine + 2-oxoglutarate
-
Escherichia coli 3-(3-iodophenyl)-2-oxopropanoate + L-glutamate
-
?
3-iodo-L-tyrosine + oxaloacetate
-
Escherichia coli 3-(3-iodophenyl)-2-oxopropanoate + L-aspartate
-
?
aspartate + 2-oxoglutarate weak activity Escherichia coli oxaloacetate + L-glutamate
-
r
L-leucine + 2-oxoglutarate weak activity Escherichia coli 4-methyl-2-oxopentanoate + L-glutamate
-
?
L-methionine + 2-oxoglutarate weak activity Escherichia coli 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate
-
Escherichia coli phenylpyruvate + L-glutamate
-
?
L-phenylalanine + oxaloacetate
-
Escherichia coli phenylpyruvate + L-aspartate
-
?
L-tryptophan + 2-oxoglutarate weak activity Escherichia coli 3-indole-2-oxopropanoate + L-glutamate
-
r
L-tryptophan + oxaloacetate
-
Escherichia coli 3-indole-2-oxopropanoate + L-aspartate
-
?
L-tyrosine + 2-oxoglutarate
-
Escherichia coli p-hydroxyphenylpyruvate + L-glutamate
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
5% loss of activity after 5 min in the presence of 0.2 mM pyridoxal 5'-phosphate and 2 mM 2-oxoglutarate, 60% loss of activity in the absence of cofactor and substrate after 5 min, more than 90% after 10 min Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
152
-
L-phenylalanine pH 7.3, 37°C Escherichia coli
250
-
L-tryptophan pH 7.3, 37°C Escherichia coli
278
-
2-oxoglutarate pH 7.3, 37°C Escherichia coli
333
-
L-tyrosine pH 7.3, 37°C Escherichia coli
500
-
oxaloacetate pH 7.3, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
tryptophan Escherichia coli
8
-
with tyrosine Escherichia coli
8
-
with phenylalanine Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal phosphate protein Escherichia coli